[ Objective ] The paper was to investigate the content of 1,2-propanediol in silage feed. [ Method ] Whole-crop corn was stored in laboratory bag silos and outdoor bunker silos. Bag silos were stored for 3, 7, 14, 28,...[ Objective ] The paper was to investigate the content of 1,2-propanediol in silage feed. [ Method ] Whole-crop corn was stored in laboratory bag silos and outdoor bunker silos. Bag silos were stored for 3, 7, 14, 28, 56 and 120 d, respectively, and fermentation products were analyzed after opening; the fermentation products, which were from three 500 t above-ground outdoor bunker silos stored for 120 d, were detected. Eight sampling points were selected for each bunker silo. [ Result] No 1,2-propanediol was detected out in laboratory bag silos, and lactic acid was dominant in fermentation products. The acetic acid content significantly increased （ P 〈 0.05 ） with the extension of storage time, whereas the acetic acid content was kept below one-third of the lactic acid content ; 1,2-propanediol and a small amount of 1-propanol were detected out in three bunker silos, and lactic acid was dominant in fermentation products, whereas the content of acetic acid was slightly lower than that of lactic acid. The differences in fermentation products from different silos and different sites in silos presented heterogeneity; except to propanediol, lactic acid bacteria and yeast, there was no significant difference in fermentation products at the top and bottom layers of silos （ P 〉0.05 ） ; there were significant differences in contents of lactic acid, acetic acid, 1,2-propanediol, and yeast between the outer and inner parts of silos （P 〈 0.05 ） ; except to pH values and acetic acid, there were significant differences in fermentation products between hunker silos （P 〈0. 05）. [ Conclusion] No 1,2-propanediol is detected out in laboratory bag silos due to relatively simple fermentation products. However, 1,2-propanediol can be detected in the bunker silos due to complex outdoor environment for silage, and the microorganisms producing 1,2-propanediol exist in silos.展开更多
以中北青贮410玉米(Zea mays L.)和秣食豆(Glycine max Merrill)为青贮材料,设置单贮全株玉米、单贮秣食豆、80%全株玉米和20%秣食豆混贮、70%全株玉米和30%秣食豆混贮、60%全株玉米和40%秣食豆混贮5个处理组,研究全株玉米和秣食豆混合...以中北青贮410玉米(Zea mays L.)和秣食豆(Glycine max Merrill)为青贮材料,设置单贮全株玉米、单贮秣食豆、80%全株玉米和20%秣食豆混贮、70%全株玉米和30%秣食豆混贮、60%全株玉米和40%秣食豆混贮5个处理组,研究全株玉米和秣食豆混合青贮的青贮效果以及最佳比例。结果表明:单贮全株玉米的发酵品质最好,单贮秣食豆的发酵品质最差。秣食豆添加比例为20%和30%的发酵品质效果相似,比秣食豆添加比例为40%的混贮效果好;就化学成分而言,单贮全株玉米的青贮效果较差,单贮秣食豆的青贮效果最好,其蛋白质含量最高(P<0.05),中性洗涤纤维和酸性洗涤纤维含量最低(P<0.05),3组混贮中秣食豆的添加比例越高效果越好。5个青贮处理综合比较发酵品质和化学成分,以70%全株玉米和30%秣食豆混合青贮的效果最好,秣食豆添加比例为20%和40%的混合青贮效果次之。展开更多
凝结芽孢杆菌(B)、植物乳杆菌(L)、粪链球菌(S)和乳酸片球菌(P)是四种同型发酵乳酸菌,为探讨其作为青贮添加剂的组合应用效果,本研究以任意三种组成的4种复合菌剂(BLS、BSP、BLP和LSP)为研究对象,进行全株玉米青贮,分别设置50 m L/t和10...凝结芽孢杆菌(B)、植物乳杆菌(L)、粪链球菌(S)和乳酸片球菌(P)是四种同型发酵乳酸菌,为探讨其作为青贮添加剂的组合应用效果,本研究以任意三种组成的4种复合菌剂(BLS、BSP、BLP和LSP)为研究对象,进行全株玉米青贮,分别设置50 m L/t和100 m L/t两个剂量处理,对照组以蒸馏水代替复合微生物菌剂。结果表明:(1)复合微生物菌剂处理全株玉米青贮在感官评价上均优于对照组;(2)与对照组相比,复合微生物菌剂处理组具有较高的干物质、粗蛋白质和淀粉含量以及较低的灰分、NDF和ADF含量,在一定程度上减少了全株玉米青贮的营养损失;(3)复合微生物处理组虽在一定程度上增加了乳酸在总有机酸中所占比例而减少了乙酸、丙酸和丁酸所占比例,但其差异未达显著水平;(4)运用隶属函数法进行综合比较,复合微生物菌剂以BLP即凝结芽孢杆菌、植物乳杆菌和乳酸片球菌组合为最佳,剂量以100 m L/t为佳。展开更多
基金Supported by National Natural Science Foundation of China(31402136)Research Project of Bureau of Reclamation in Heilongjiang Province(HNK135-04-02-03)+1 种基金Postdoctoral Fund in Heilongjiang Province(LBH-Z14029)Doctoral Fund in Heilongjiang Bayi Agricultural University(B2012-06)
文摘[ Objective ] The paper was to investigate the content of 1,2-propanediol in silage feed. [ Method ] Whole-crop corn was stored in laboratory bag silos and outdoor bunker silos. Bag silos were stored for 3, 7, 14, 28, 56 and 120 d, respectively, and fermentation products were analyzed after opening; the fermentation products, which were from three 500 t above-ground outdoor bunker silos stored for 120 d, were detected. Eight sampling points were selected for each bunker silo. [ Result] No 1,2-propanediol was detected out in laboratory bag silos, and lactic acid was dominant in fermentation products. The acetic acid content significantly increased （ P 〈 0.05 ） with the extension of storage time, whereas the acetic acid content was kept below one-third of the lactic acid content ; 1,2-propanediol and a small amount of 1-propanol were detected out in three bunker silos, and lactic acid was dominant in fermentation products, whereas the content of acetic acid was slightly lower than that of lactic acid. The differences in fermentation products from different silos and different sites in silos presented heterogeneity; except to propanediol, lactic acid bacteria and yeast, there was no significant difference in fermentation products at the top and bottom layers of silos （ P 〉0.05 ） ; there were significant differences in contents of lactic acid, acetic acid, 1,2-propanediol, and yeast between the outer and inner parts of silos （P 〈 0.05 ） ; except to pH values and acetic acid, there were significant differences in fermentation products between hunker silos （P 〈0. 05）. [ Conclusion] No 1,2-propanediol is detected out in laboratory bag silos due to relatively simple fermentation products. However, 1,2-propanediol can be detected in the bunker silos due to complex outdoor environment for silage, and the microorganisms producing 1,2-propanediol exist in silos.
文摘以中北青贮410玉米(Zea mays L.)和秣食豆(Glycine max Merrill)为青贮材料,设置单贮全株玉米、单贮秣食豆、80%全株玉米和20%秣食豆混贮、70%全株玉米和30%秣食豆混贮、60%全株玉米和40%秣食豆混贮5个处理组,研究全株玉米和秣食豆混合青贮的青贮效果以及最佳比例。结果表明:单贮全株玉米的发酵品质最好,单贮秣食豆的发酵品质最差。秣食豆添加比例为20%和30%的发酵品质效果相似,比秣食豆添加比例为40%的混贮效果好;就化学成分而言,单贮全株玉米的青贮效果较差,单贮秣食豆的青贮效果最好,其蛋白质含量最高(P<0.05),中性洗涤纤维和酸性洗涤纤维含量最低(P<0.05),3组混贮中秣食豆的添加比例越高效果越好。5个青贮处理综合比较发酵品质和化学成分,以70%全株玉米和30%秣食豆混合青贮的效果最好,秣食豆添加比例为20%和40%的混合青贮效果次之。
文摘凝结芽孢杆菌(B)、植物乳杆菌(L)、粪链球菌(S)和乳酸片球菌(P)是四种同型发酵乳酸菌,为探讨其作为青贮添加剂的组合应用效果,本研究以任意三种组成的4种复合菌剂(BLS、BSP、BLP和LSP)为研究对象,进行全株玉米青贮,分别设置50 m L/t和100 m L/t两个剂量处理,对照组以蒸馏水代替复合微生物菌剂。结果表明:(1)复合微生物菌剂处理全株玉米青贮在感官评价上均优于对照组;(2)与对照组相比,复合微生物菌剂处理组具有较高的干物质、粗蛋白质和淀粉含量以及较低的灰分、NDF和ADF含量,在一定程度上减少了全株玉米青贮的营养损失;(3)复合微生物处理组虽在一定程度上增加了乳酸在总有机酸中所占比例而减少了乙酸、丙酸和丁酸所占比例,但其差异未达显著水平;(4)运用隶属函数法进行综合比较,复合微生物菌剂以BLP即凝结芽孢杆菌、植物乳杆菌和乳酸片球菌组合为最佳,剂量以100 m L/t为佳。
