AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine...AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the "bubbling" cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P＜0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes.展开更多
There were more expounding to north—west (west) trend fault and north\|east trend fault within Qiangtang Basin, North Part of Tibet, in the past literature. With increasing of geophysical exploration data, nearly eas...There were more expounding to north—west (west) trend fault and north\|east trend fault within Qiangtang Basin, North Part of Tibet, in the past literature. With increasing of geophysical exploration data, nearly east\|west trend structure began to be taken note to. Since the year of 1995, by a synthetic study to geophysical and geological data, that south\|north trend faulted structures are well developed. These structures should be paid much more attention to, because they have important theoretical meaning and practical significance.1 Spreading of south\|north faulted structure belt According to different geological and geophysical data, the six larger scale nearly south\|north faulted structure belt could be distinguished within the scope of east longitude 84°～96° and near Qiangtang Basin. The actual location of the six belts are nearly located in the west of the six meridian of east longitude 85°,87°,89°,91°,93°,95° or located near these meridian. The six south\|north faulted structure belts spread in the same interval with near 2° longitude interval. The more clear and much more significance of south\|north trend faulted structure belts are the two S—N trend faulted structure belts of east longitude 87° and 89°. There are S—N trend faulted structure belts in the west of east longitude 83°,81°, or near the longitudes. The structure belts spreading features,manifestation,geological function and its importance, and inter texture and structure are not exactly so same. The structure belts all different degree caused different region of geological structure or gravity field and magnetic field. There is different scale near S—N trend faulted structure belt between the belts.展开更多
A searching-machining system of RL & SM (Rapid Location and State Memory) universal fixture is here introduced, and the concept of rapid searching technology and manufacturing information transformation is then de...A searching-machining system of RL & SM (Rapid Location and State Memory) universal fixture is here introduced, and the concept of rapid searching technology and manufacturing information transformation is then described, with the appropriate control methods and key techniques for its realization being proposed and also practically realized. Theoretical analysis and experimental results show that the proposed idea and methods are feasible to serve as in the practical application of the RL & SM system.展开更多
文摘AIM To extract and purify the transforming growth factor β (TGF β), and to demonstrate its biological activity in vivo and induction of apoptosis of hepatocytes in vitro.METHODS TGF β was isolated from fresh bovine platelets by acid/ethanol extraction method and purified with ion exchange and gel chromatography. The extracted TGF β was injected subcutaneously to mice, and its biological activity in vivo was observed 72 hfs post-injection by HE staining. The morphological changes were observed by HE staining and the occurrence of apoptosis was detected by TUNEL method after the human normal hepatic cell line QZG was treated with 8 μg@L 1 TGFβ for 12 hrs in vitro.RESULTS The molecular mass 25 ku TGF β protein was successfully extracted. It was able to induce localized granulation tissue formation in vivo. TGF β-treated hepatocytes showed obvious apoptotic morphological changes, including the pyknosis and dense-stained nuclei and cytoplasm, the fragmentary, annular or crescent nuclei, and the "bubbling" cytoplasm. Moreover, its apoptotic rate was significantly higher than that of the control group (P＜0.05).CONCLUSION Biological active TGF β protein is extracted and purified successfully from bovine platelets, and it is able to induce the apoptosis of hepatocytes.
文摘There were more expounding to north—west (west) trend fault and north\|east trend fault within Qiangtang Basin, North Part of Tibet, in the past literature. With increasing of geophysical exploration data, nearly east\|west trend structure began to be taken note to. Since the year of 1995, by a synthetic study to geophysical and geological data, that south\|north trend faulted structures are well developed. These structures should be paid much more attention to, because they have important theoretical meaning and practical significance.1 Spreading of south\|north faulted structure belt According to different geological and geophysical data, the six larger scale nearly south\|north faulted structure belt could be distinguished within the scope of east longitude 84°～96° and near Qiangtang Basin. The actual location of the six belts are nearly located in the west of the six meridian of east longitude 85°,87°,89°,91°,93°,95° or located near these meridian. The six south\|north faulted structure belts spread in the same interval with near 2° longitude interval. The more clear and much more significance of south\|north trend faulted structure belts are the two S—N trend faulted structure belts of east longitude 87° and 89°. There are S—N trend faulted structure belts in the west of east longitude 83°,81°, or near the longitudes. The structure belts spreading features,manifestation,geological function and its importance, and inter texture and structure are not exactly so same. The structure belts all different degree caused different region of geological structure or gravity field and magnetic field. There is different scale near S—N trend faulted structure belt between the belts.
文摘A searching-machining system of RL & SM (Rapid Location and State Memory) universal fixture is here introduced, and the concept of rapid searching technology and manufacturing information transformation is then described, with the appropriate control methods and key techniques for its realization being proposed and also practically realized. Theoretical analysis and experimental results show that the proposed idea and methods are feasible to serve as in the practical application of the RL & SM system.
