Objective: To determine the occurrence of Chlamydia spp.in wild birds in Thailand.Methods: Cloacal and tracheal swabs of 313 wild birds from 11 orders, 27 families, and 51 species were tested to determine the occurren...Objective: To determine the occurrence of Chlamydia spp.in wild birds in Thailand.Methods: Cloacal and tracheal swabs of 313 wild birds from 11 orders, 27 families, and 51 species were tested to determine the occurrence of Chlamydia infection.The outer membrane protein A(ompA) gene was amplified from positive samples to construct a phylogenetic tree.Results: At the time of sample collection, none of the birds showed clinical signs of any disease.Of 313 wild birds, two Asian openbill stork(Anastomus oscitans) were positive for Chlamydia spp., representing 0.64%(2/313) and 4.9%(2/41) occurrence for birds overall and for the Asian openbill stork, respectively.Phylogram analysis based on deduced amino acid of the ompA gene showed that Chlamydia spp.in Asian openbill storks was closely related to that in wildfowl(Pica pica and Cygnus olor) from Poland in a different branch with a 95% bootstrap value and had a shorter evolutionary distance to Chlamydia abortus.Conclusions: Asymptomatic Asian openbill storks could be a potential source of Chlamydia infection in domestic animals, poultry, and humans who share their habitat.展开更多
植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用...植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。展开更多
We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kerne...We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kernel weight and oil parameters. Accessions ranged from0.20 to 1.5 g in kernel weight, 0.2–3.0 mm in shell thickness, and 16–55 % in oil content. The predominant vegetable oil components of kernels were 4.6–9.5 % palmitic acid, 0.4–0.8 % palmitoleic acid, 1.0–3.4 % stearic acid,48.8–88.4 % oleic acid and 11.3–33.2 % linoleic acid.Linolenic acid was detected in 15 accessions. High heritability was recorded for all studied traits and was maximum for shell thickness（98.5 %） and minimum for oil content（97.1 %）. Maximum and minimum ‘Euclidean＇pair wise dissimilarities were 17.9 and 0.5, respectively.All 40 accessions were grouped into two major clusters.展开更多
基金financially supported by the Faculty of Veterinary Science,Mahidol Universitycollected by the project of Establishment of zoonotic viral networking system:developmental phase+1 种基金subproject of Influenza A virus surveys in migratory and residence birds of Thailand granting from Cluster and Program Management Office(P-15-50535)the National Science and Technology Development Agency,Thailand
文摘Objective: To determine the occurrence of Chlamydia spp.in wild birds in Thailand.Methods: Cloacal and tracheal swabs of 313 wild birds from 11 orders, 27 families, and 51 species were tested to determine the occurrence of Chlamydia infection.The outer membrane protein A(ompA) gene was amplified from positive samples to construct a phylogenetic tree.Results: At the time of sample collection, none of the birds showed clinical signs of any disease.Of 313 wild birds, two Asian openbill stork(Anastomus oscitans) were positive for Chlamydia spp., representing 0.64%(2/313) and 4.9%(2/41) occurrence for birds overall and for the Asian openbill stork, respectively.Phylogram analysis based on deduced amino acid of the ompA gene showed that Chlamydia spp.in Asian openbill storks was closely related to that in wildfowl(Pica pica and Cygnus olor) from Poland in a different branch with a 95% bootstrap value and had a shorter evolutionary distance to Chlamydia abortus.Conclusions: Asymptomatic Asian openbill storks could be a potential source of Chlamydia infection in domestic animals, poultry, and humans who share their habitat.
文摘植物光系统Ⅰ反应中心亚基Ⅴ(photosystemⅠreaction center subunitⅤ,简称PSAG或PSⅠ-G)是光合系统Ⅰ的主要组件,具有维持PSⅠ复合体稳定性的重要作用,并与抗盐密切相关。本研究以福州宦溪野生蕉(Musa spp.AB group)叶片为材料,采用同源克隆的方法 ,首次分离到PSAG基因的2个成员:PSAG1、PSAG2(GenBank登录号分别为JX317082、JX317083),分别为800、827 bp,分别编码150、160个氨基酸;PSAG1、PSAG2的基因组序列分析表明2个成员均没有内含子。生物信息学分析表明:PSAG1、PSAG2具有PSⅠ的Ⅹ亚基超家族(photosystemⅠreaction center subunitⅩpsaK)保守结构域,是不具有信号肽的跨膜蛋白,具有亲水性;PSAG1、PSAG2均有4个位点发生磷酸化。宦溪野生蕉PSAG在进化过程中形成了特殊的结构特征,即PSAG1和PSAG2没有内含子,并且在不同物种间保守区具有高度的一致性,为保持PSAG功能的稳定性提供了重要保证。
基金financially supported by Payam-e-Noor University
文摘We assessed chemical composition and variation in oil content and seed weight of 40 wild-growing almonds（Prunus L. spp.） accessions collected from different parts of Iran. There were significant differences in kernel weight and oil parameters. Accessions ranged from0.20 to 1.5 g in kernel weight, 0.2–3.0 mm in shell thickness, and 16–55 % in oil content. The predominant vegetable oil components of kernels were 4.6–9.5 % palmitic acid, 0.4–0.8 % palmitoleic acid, 1.0–3.4 % stearic acid,48.8–88.4 % oleic acid and 11.3–33.2 % linoleic acid.Linolenic acid was detected in 15 accessions. High heritability was recorded for all studied traits and was maximum for shell thickness（98.5 %） and minimum for oil content（97.1 %）. Maximum and minimum ‘Euclidean＇pair wise dissimilarities were 17.9 and 0.5, respectively.All 40 accessions were grouped into two major clusters.
