Antibiotic resistance genes(ARGs) as emergence contaminations have spread widely in the water environment. Wild fish may be recipients and communicators of ARGs in the water environment, however, the distribution and ...Antibiotic resistance genes(ARGs) as emergence contaminations have spread widely in the water environment. Wild fish may be recipients and communicators of ARGs in the water environment, however, the distribution and transmission of ARGs in the wild fish and relevant water environment were rarely reported. Here, we have profiled ARGs and bacterial communities in wild freshwater fish and relevant water in a peri-urban river using highthroughput qPCR and 16 S rRNA gene sequence. A total of 80 and 220 unique ARG subtypes were identified in fish and water samples. Fish and water both showed significant ARG seasonal variations( P < 0.05). The highest absolute abundance of ARGs in fish and water occurred in summer(1.32 × 10^(9) copies per g, on average) and autumn(9.04 × 10^(6) copies per mL), respectively. In addition, the bipartite network analysis showed that 9 ARGs and 1 mobile genetic element continuously shared in fish and water. Furthermore, bacteria shared in fish and water were found to significantly correlate with shard ARGs. The findings demonstrate that bacteria and ARGs in fish and water could interconnect and ARGs might transfer between fish and water using bacteria as a spreading medium.展开更多
Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that dif...Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that differ in their proximate composition, amino acids, fatty acids, and minerals. Wild fish had a significantly lower crude lipid contents than cultured fish (P P ∑PUFAs) showed an obviously opposite trend. As compared with cultured fish, wild fish had significantly higher levels of n-3 PUFAs, arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahxaenoic acid (DHA) (P P > 0.05), except for Na, Fe and Se. In conclusion, diet composition and external aqueous environment may determine the differences between wild and cultured topmouth culter.展开更多
基金supported by the Natural Science Foundation of China (Nos. 21876147 and 21677121)。
文摘Antibiotic resistance genes(ARGs) as emergence contaminations have spread widely in the water environment. Wild fish may be recipients and communicators of ARGs in the water environment, however, the distribution and transmission of ARGs in the wild fish and relevant water environment were rarely reported. Here, we have profiled ARGs and bacterial communities in wild freshwater fish and relevant water in a peri-urban river using highthroughput qPCR and 16 S rRNA gene sequence. A total of 80 and 220 unique ARG subtypes were identified in fish and water samples. Fish and water both showed significant ARG seasonal variations( P < 0.05). The highest absolute abundance of ARGs in fish and water occurred in summer(1.32 × 10^(9) copies per g, on average) and autumn(9.04 × 10^(6) copies per mL), respectively. In addition, the bipartite network analysis showed that 9 ARGs and 1 mobile genetic element continuously shared in fish and water. Furthermore, bacteria shared in fish and water were found to significantly correlate with shard ARGs. The findings demonstrate that bacteria and ARGs in fish and water could interconnect and ARGs might transfer between fish and water using bacteria as a spreading medium.
文摘Research was conducted on topmouth culter (Culter alburnus) grown in ponds and lakes as well as wild types in order to determine their chemical composition and nutritional value. There are three types of fish that differ in their proximate composition, amino acids, fatty acids, and minerals. Wild fish had a significantly lower crude lipid contents than cultured fish (P P ∑PUFAs) showed an obviously opposite trend. As compared with cultured fish, wild fish had significantly higher levels of n-3 PUFAs, arachidonic acid (AA), eicosapentaenoic acid (EPA) and docosahxaenoic acid (DHA) (P P > 0.05), except for Na, Fe and Se. In conclusion, diet composition and external aqueous environment may determine the differences between wild and cultured topmouth culter.
