BACKGROUND The vaginal microbiome plays a critical role in the health of pregnant women and their newborns.Group B Streptococcus(GBS)and vaginal cleanliness significantly affect the vaginal microecosystem and are clos...BACKGROUND The vaginal microbiome plays a critical role in the health of pregnant women and their newborns.Group B Streptococcus(GBS)and vaginal cleanliness significantly affect the vaginal microecosystem and are closely associated with vaginal diseases.AIM To explore the effects of GBS status and vaginal cleanliness on vaginal microecosystems.METHODS We collected 160 vaginal swabs from pregnant women and divided them into the following four groups based on GBS status and vaginal cleanliness:GBS-positive+vaginal cleanliness I–II degree,GBS-negative+vaginal cleanliness I–II degree,GBS-positive+vaginal cleanliness III–IV degree,and GBS-negative+vaginal cleanliness III–IV degree.Samples were subjected to 16S rRNA gene amplicon sequencing.RESULTS Alpha diversity analysis showed that the Shannon index did not significantly differ between the four groups.We identified significant variation in taxa abundance between the GBS-positive and GBS-negative groups and between the vaginal cleanliness I–II degree and III–IV degree groups.Principal coordinate analysis and non-metric multidimensional scaling analysis further confirmed the microbial diversity of the four groups.Moreover,the linear discriminant analysis demonstrated that Lactobacillus jensenii and Actinobacteria were strongly associated with GBS-positive status,and Lactobacillus iners,Lactobacillaceae,Lactobacillus,Lactobacillales,Bacilli and Firmicutes were closely correlated with GBS-negative status.CONCLUSION GBS status and vaginal cleanliness significantly affect vaginal microbiome differences in pregnant women.Our findings provide instructional information for clinical antibiotic treatment in pregnant women with different GBS statuses and vaginal cleanliness degrees.展开更多
1.This study is one of The Applied Economic Institutes Linkages Project(AERIL)which is funded by theCanadian International Development Agency(CIDA)and jiontly managed by The Conference Board of Canada(CBOC)and the Int...1.This study is one of The Applied Economic Institutes Linkages Project(AERIL)which is funded by theCanadian International Development Agency(CIDA)and jiontly managed by The Conference Board of Canada(CBOC)and the International Trade Research Institute in China.It is jointly implemented by the Institute ofEconomic Research of the Chinese State Planning Commission and the North—South Institute of Canada。展开更多
With the rapid development of China’s cultural and creative industries,the influence of cultural and creative ideas in fashion design is increasing,and art talents with innovative ability have become hot objects in t...With the rapid development of China’s cultural and creative industries,the influence of cultural and creative ideas in fashion design is increasing,and art talents with innovative ability have become hot objects in the society.As the cradle of fashion design talents training and output,colleges and universities in our country shoulder the important task of training fashion design talents in the new period.In the current clothing design curriculum reform,school enterprise cooperation and collaborative innovation have become an important development direction.Under this background,this paper analyzes the teaching reform path of women’s clothing design course in Colleges and universities.展开更多
文摘BACKGROUND The vaginal microbiome plays a critical role in the health of pregnant women and their newborns.Group B Streptococcus(GBS)and vaginal cleanliness significantly affect the vaginal microecosystem and are closely associated with vaginal diseases.AIM To explore the effects of GBS status and vaginal cleanliness on vaginal microecosystems.METHODS We collected 160 vaginal swabs from pregnant women and divided them into the following four groups based on GBS status and vaginal cleanliness:GBS-positive+vaginal cleanliness I–II degree,GBS-negative+vaginal cleanliness I–II degree,GBS-positive+vaginal cleanliness III–IV degree,and GBS-negative+vaginal cleanliness III–IV degree.Samples were subjected to 16S rRNA gene amplicon sequencing.RESULTS Alpha diversity analysis showed that the Shannon index did not significantly differ between the four groups.We identified significant variation in taxa abundance between the GBS-positive and GBS-negative groups and between the vaginal cleanliness I–II degree and III–IV degree groups.Principal coordinate analysis and non-metric multidimensional scaling analysis further confirmed the microbial diversity of the four groups.Moreover,the linear discriminant analysis demonstrated that Lactobacillus jensenii and Actinobacteria were strongly associated with GBS-positive status,and Lactobacillus iners,Lactobacillaceae,Lactobacillus,Lactobacillales,Bacilli and Firmicutes were closely correlated with GBS-negative status.CONCLUSION GBS status and vaginal cleanliness significantly affect vaginal microbiome differences in pregnant women.Our findings provide instructional information for clinical antibiotic treatment in pregnant women with different GBS statuses and vaginal cleanliness degrees.
文摘1.This study is one of The Applied Economic Institutes Linkages Project(AERIL)which is funded by theCanadian International Development Agency(CIDA)and jiontly managed by The Conference Board of Canada(CBOC)and the International Trade Research Institute in China.It is jointly implemented by the Institute ofEconomic Research of the Chinese State Planning Commission and the North—South Institute of Canada。
文摘With the rapid development of China’s cultural and creative industries,the influence of cultural and creative ideas in fashion design is increasing,and art talents with innovative ability have become hot objects in the society.As the cradle of fashion design talents training and output,colleges and universities in our country shoulder the important task of training fashion design talents in the new period.In the current clothing design curriculum reform,school enterprise cooperation and collaborative innovation have become an important development direction.Under this background,this paper analyzes the teaching reform path of women’s clothing design course in Colleges and universities.
