This article explores the use of social networks by workers in Abidjan, Côte d’Ivoire, with particular emphasis on a descriptive or quantitative analysis aimed at understanding motivations and methods of use. Mo...This article explores the use of social networks by workers in Abidjan, Côte d’Ivoire, with particular emphasis on a descriptive or quantitative analysis aimed at understanding motivations and methods of use. More than five hundred and fifty questionnaires were distributed, highlighting workers’ preferred digital channels and platforms. The results indicate that the majority use social media through their mobile phones, with WhatsApp being the most popular app, followed by Facebook and LinkedIn. The study reveals that workers use social media for entertainment purposes and to develop professional and social relationships, with 55% unable to live without social media at work for recreational activities. In addition, 35% spend on average 1 to 2 hours on social networks, mainly between 12 p.m. and 2 p.m. It also appears that 46% believe that social networks moderately improve their productivity. These findings can guide marketing strategies, training, technology development and government policies related to the use of social media in the workplace.展开更多
The phenomenon of Cenozoic migrant worker shortage is a realism problem of China's enterprise, and Cenozoic migrant worker has become an important force occupied the social structure. So the study of Cenozoic migr...The phenomenon of Cenozoic migrant worker shortage is a realism problem of China's enterprise, and Cenozoic migrant worker has become an important force occupied the social structure. So the study of Cenozoic migrant worker's status and the demand is imperative. Understand employees' both psychological and physiological satisfaction of enterprises' environmental factors, and make some corresponding changes is of great help to reduce the employee turnover rate. This paper commenced to research from the Cenozoic migrant worker 's characteristics, analyzed conditions and puts forward the corresponding countermeasures.展开更多
Objectives Tumor necrosis factor-α (TNF-α) may play an important role in host's immune response to mycobacterium tuberculosis (M. tuberculosis) infection. This study was to investigate the association of TNF-α...Objectives Tumor necrosis factor-α (TNF-α) may play an important role in host's immune response to mycobacterium tuberculosis (M. tuberculosis) infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis (TB) among patients with coal worker's pneumoconiosis (CWP). Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism (PCR-RFLP). Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher's exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls (2,2=5.44, P=-0.07). But difference in frequency of the TNF-α-308 A allele was identified between them (2,2-5.14, P=0.02). No significant difference in frequencies of the TNF-α-238 genotype and allele (P=0.23 and P=0.09, respectively) was found between cases and controls either, with combined (GG and AA) OR of 3.96 (95% confidence interval of 1.30-12.09) at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 (95% CI of 1.06-3.71) for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index (OR=4.92), as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise.展开更多
In the United States,an unexpected and severe increase in coal miners’lung diseases in the late 1990s prompted researchers to investigate the causes of the disease resurgence.This study aims to scrutinize the effects...In the United States,an unexpected and severe increase in coal miners’lung diseases in the late 1990s prompted researchers to investigate the causes of the disease resurgence.This study aims to scrutinize the effects of various mining parameters,including coal rank,mine size,mine operation type,coal seam height,and geographical location on the prevalence of coal worker’s pneumoconiosis(CWP)in surface and underground coal mines.A comprehensive dataset was created using the U.S.Mine Safety and Health Administration(MSHA)Employment and Accident/Injury databases.The information was merged based on the mine ID by utilizing SQL data management software.A total number of 123,589 mine-year observations were included in the statistical analysis.Generalized Estimating Equation(GEE)model was used to conduct a statistical analysis on a total of 29,707,and 32,643 mine-year observations for underground and surface coal mines,respectively.The results of the econometrics approach revealed that coal workers in underground coal mines are at a greater risk of CWP comparing to those of surface coal operations.Furthermore,underground coal mines in the Appalachia and Interior regions are at a higher risk of CWP prevalence than the Western region.Surface coal mines in the Appalachian coal region are more likely to CWP development than miners in the Western region.The analysis also indicated that coal workers working in smaller mines are more vulnerable to CWP than those in large mine sizes.Furthermore,coal workers in thin-seam underground mine operations are more likely to develop CWP.展开更多
<strong>Background:</strong> There are various health problems in rotating shift workers. There have been few studies on the associations of male climacteric symptoms with duration of working and lifestyle...<strong>Background:</strong> There are various health problems in rotating shift workers. There have been few studies on the associations of male climacteric symptoms with duration of working and lifestyle. <strong>Objective: </strong>The aim of this study was to determine the differences in male climacteric symptoms depending on the number of working years and to determine whether lifestyle habits differ depending on the number of working years in rotating shift workers. <strong>Methods:</strong> We collected participant’s data from the manufacturing companies in Japan during the period from March to May in 2017. We conducted a self-administered questionnaire survey in 1561 male rotating night shift workers. Male climacteric symptoms were evaluated by using the Aging Males’ Symptoms (AMS) rating scale. We analyzed 636 rotating shift workers aged over 40 years old whose all AMS data was collected. <strong>Results:</strong> A significant difference in psychological AMS score was found between men who had worked for 10 - 19 years (9.4 ± 3.9) and men who had worked for more than 40 years (7.6 ± 3.0) (p = 0.011). Sexual functional AMS score significantly differed depending on the number of working years (less than 10 years: 8.1 ± 3.9, 10 - 19 years: 9.8 ± 4.1, 20 - 29 years: 7.9 ± 3.3, 30 - 39 years: 9.3 ± 3.5, more than 40 years: 9.3 ± 3.5) (p < 0.001). The proportion of workers with a balanced meal intake was significantly lower in men who had worked for less than 10 years and the proportions of such workers were significantly higher in men who had worked for 10 - 19 years and for more than 40 years. The proportions of men who did not drink alcohol were high in men who had worked for less than 10 years and 20 - 29 years and low in men who had worked more than 30 years. <strong>Conclusion: </strong>Psychological AMS score and sexual functional AMS score are significantly different among the 5 groups according to the number of working years on rotating shift. In addition, well-balanced diet on day shift and reduction of alcohol drinking is a related-factor for long-term rotating night shift workers.展开更多
Studies on long-term wage levels of rural migrant workers are rarely found in relevant literature. On the basis of systematic collection of statistical data, this paper gives a quantitative estimation of the wage leve...Studies on long-term wage levels of rural migrant workers are rarely found in relevant literature. On the basis of systematic collection of statistical data, this paper gives a quantitative estimation of the wage level of rural migrant workers in China and its evolution following pro market reforms since 1978. Results indicate that over the past 30 years, the nominal monetary wage of migrant workers maintained a 10% average annual increase. Real wage levels adjusted by the consumer price index (CPI) experienced three stages of evolution: initially, migrant workers earned a higher average monetary salary than formal employees, whereas this situation reversed in later stages.展开更多
At present time, there has been a demand for management systems that can survey and monitor a PC (personal computer) practice room, movement of people in an office, situation of utilization of facilities and so on i...At present time, there has been a demand for management systems that can survey and monitor a PC (personal computer) practice room, movement of people in an office, situation of utilization of facilities and so on in real time without causing psychological stress. For example, PC administrators in Japanese national educational institutions must report operation status of PC practice rooms once a year. But, there is currently no system for automatically recording PC operating situations. Therefore, the burden on the PC administrators is big. In this study, we aimed at systems for accurately managing the sitting and work time without psychologically stressing PC users. This time, we propose uniform management systems of sitting and work time using smart tap node and mat sensor node by IoT (Internet of Things) technology. The smart tap was connected to PCs to acquire the operating status of the PCs. In parallel with this smart tap, the mat sensor was used to acquire human presence state. By calculating binary data representing the PC operating status and human presence state from the two sensing data (smart tap and mat sensor) by the proposed technique, we can exactly calculate wasteful power consumption etc. The use of IoT technology makes it unnecessary to use large installation services when introducing our system. Therefore, this our proposal system can be easily installed even by unskilled workers.展开更多
Amy Tan, a Chinese American writer describes the stories between four pairs of mothers and daughters in The Joy Luck Club. Four mothers have different growing backgrounds as their daughters. This essay will analyze th...Amy Tan, a Chinese American writer describes the stories between four pairs of mothers and daughters in The Joy Luck Club. Four mothers have different growing backgrounds as their daughters. This essay will analyze the reasons behind the mother-daughter's conflicts from the perspectives of the differences of eastern and western culture, mothers' educational methods and excessively high expectation of the mothers.展开更多
The toxicity of benzene is well known, and its leukemia effect has established. It is a natural constituent of crude oil and the diseases related to its exposure are recognized as occupational diseases. <strong>...The toxicity of benzene is well known, and its leukemia effect has established. It is a natural constituent of crude oil and the diseases related to its exposure are recognized as occupational diseases. <strong>Objective:</strong> To assess occupational exposure of benzene to workers in an oil and gas production company. <strong>Materials and Methods:</strong> Firstly, it was a descriptive, cross-sectional study which consisted of benzene atmospheric quantification in a sample individual measurement of a homogeneous exposure group of workers. Secondly, urinary assays of S-phenylmercapturic acid have been performed at the end of the shift in the selected workers.<strong> Results:</strong> The study has included 79 (47.88%) workers, 17 atmospheric samples were usable and 79 urinary assays at the end of the shift were performed. The average benzene concentration for all sites was 10 times lower than the regulatory average exposure value (1 ppm = 3.2 mg/m<sup>3</sup>): average: 0.122 pp, median: 0.053 ppm and range: 0.019 - 1.448 ppm. All 79 urinary assays of S-phenylmercapturic acid with a biological exposure index of less than 25 μg/g creatinine: mean: 0.70 μg/g creatinine, median: 0.52 μg/g creatinine and extends: 0.23 to 6.7 μg/g creatinine. <strong>Conclusion:</strong> Exposure was below to the limit value for benzene in both atmospheric metrology and biometrology. Therefore it is an occupational group with low exposure to benzene. Thus, the medical supervision will be adapted according to the potentially exposing tasks.展开更多
Various networks exist in the world today including biological, social, information, and communication networks with the Internet as the largest network of all. One salient structural feature of these networks is the ...Various networks exist in the world today including biological, social, information, and communication networks with the Internet as the largest network of all. One salient structural feature of these networks is the formation of groups or communities of vertices that tend to be more connected to each other within the same group than to those outside. Therefore, the detection of these communities is a topic of great interest and importance in many applications and different algorithms including label propagation have been developed for such purpose. Speaker-listener label propagation algorithm (SLPA) enjoys almost linear time complexity, so desirable in dealing with large networks. As an extension of SLPA, this study presented a novel weighted label propagation algorithm (WLPA), which was tested on four real world social networks with known community structures including the famous Zachary's karate club network. Wilcoxon tests on the communities found in the karate club network by WLPA demonstrated an improved statistical significance over SLPA. Withthehelp of Wilcoxon tests again, we were able to determine the best possible formation of two communities in this network relative to the ground truth partition, which could be used as a new benchmark for assessing community detection algorithms. Finally WLPA predicted better communities than SLPA in two of the three additional real social networks, when compared to the ground truth.展开更多
The protein composition of the egg development in the high royal jelly producing bees (Apis mellifera L.) was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal j...The protein composition of the egg development in the high royal jelly producing bees (Apis mellifera L.) was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal jelly producing worker bees (Apis mellifera L.) by using two-dimensional gel electrophoresis along with their three-day development. The results showed that 160, 195, and 176 proteins, with a wide range of molecular weight (17-80 KDa) and relatively narrow scope of pI (4. 00-8.40) could be detected on day 1, day 2, and day 3, respectively, during the developmental process of the egg. Meanwhile 44 protein spots were constantly detected along with the egg development. Among them 36% were in the uptrend along with the egg development, 14% were in the downtrend, and 39% were of the largest expressed volume on day 2. In addition, the specific proteins were expressed on day 1, day 2, and day 3 (89, 77, and 80, respectively). Besides the coexistent and specific proteins, 24 proteins were expressed on day 1 and day 2, but silenced on day 3, 49 proteins were expressed on day 2 and day 3, but silenced on day 1, only 3 proteins were expressed on day 1 and day 3, but silenced on day 2. The result indicates that egg development is a sequential and complex gene controlled process, where the eggs of day 2 express the most active proteins. The coexistent proteins suggest that it is conservative and indispensable for this event. These specific proteins suggest that the different developmental stage needs specific proteins to regulate it.展开更多
文摘This article explores the use of social networks by workers in Abidjan, Côte d’Ivoire, with particular emphasis on a descriptive or quantitative analysis aimed at understanding motivations and methods of use. More than five hundred and fifty questionnaires were distributed, highlighting workers’ preferred digital channels and platforms. The results indicate that the majority use social media through their mobile phones, with WhatsApp being the most popular app, followed by Facebook and LinkedIn. The study reveals that workers use social media for entertainment purposes and to develop professional and social relationships, with 55% unable to live without social media at work for recreational activities. In addition, 35% spend on average 1 to 2 hours on social networks, mainly between 12 p.m. and 2 p.m. It also appears that 46% believe that social networks moderately improve their productivity. These findings can guide marketing strategies, training, technology development and government policies related to the use of social media in the workplace.
文摘The phenomenon of Cenozoic migrant worker shortage is a realism problem of China's enterprise, and Cenozoic migrant worker has become an important force occupied the social structure. So the study of Cenozoic migrant worker's status and the demand is imperative. Understand employees' both psychological and physiological satisfaction of enterprises' environmental factors, and make some corresponding changes is of great help to reduce the employee turnover rate. This paper commenced to research from the Cenozoic migrant worker 's characteristics, analyzed conditions and puts forward the corresponding countermeasures.
基金supported by grants from China National Programs for Science and Technology Development (Grant No. 2003BA712A11-24)Scientific Research Fund of North China Coal Medical College (Grant No. 2005-14)
文摘Objectives Tumor necrosis factor-α (TNF-α) may play an important role in host's immune response to mycobacterium tuberculosis (M. tuberculosis) infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis (TB) among patients with coal worker's pneumoconiosis (CWP). Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism (PCR-RFLP). Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher's exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls (2,2=5.44, P=-0.07). But difference in frequency of the TNF-α-308 A allele was identified between them (2,2-5.14, P=0.02). No significant difference in frequencies of the TNF-α-238 genotype and allele (P=0.23 and P=0.09, respectively) was found between cases and controls either, with combined (GG and AA) OR of 3.96 (95% confidence interval of 1.30-12.09) at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 (95% CI of 1.06-3.71) for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index (OR=4.92), as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise.
基金This study is funded by the National Institute for Occupational Safety and Health(NIOSH)[75D30119C06390].The authors would like to thank Dr.Arvin Ebrahimkhanlou for all precise technical comments related to statistical analysis.
文摘In the United States,an unexpected and severe increase in coal miners’lung diseases in the late 1990s prompted researchers to investigate the causes of the disease resurgence.This study aims to scrutinize the effects of various mining parameters,including coal rank,mine size,mine operation type,coal seam height,and geographical location on the prevalence of coal worker’s pneumoconiosis(CWP)in surface and underground coal mines.A comprehensive dataset was created using the U.S.Mine Safety and Health Administration(MSHA)Employment and Accident/Injury databases.The information was merged based on the mine ID by utilizing SQL data management software.A total number of 123,589 mine-year observations were included in the statistical analysis.Generalized Estimating Equation(GEE)model was used to conduct a statistical analysis on a total of 29,707,and 32,643 mine-year observations for underground and surface coal mines,respectively.The results of the econometrics approach revealed that coal workers in underground coal mines are at a greater risk of CWP comparing to those of surface coal operations.Furthermore,underground coal mines in the Appalachia and Interior regions are at a higher risk of CWP prevalence than the Western region.Surface coal mines in the Appalachian coal region are more likely to CWP development than miners in the Western region.The analysis also indicated that coal workers working in smaller mines are more vulnerable to CWP than those in large mine sizes.Furthermore,coal workers in thin-seam underground mine operations are more likely to develop CWP.
文摘<strong>Background:</strong> There are various health problems in rotating shift workers. There have been few studies on the associations of male climacteric symptoms with duration of working and lifestyle. <strong>Objective: </strong>The aim of this study was to determine the differences in male climacteric symptoms depending on the number of working years and to determine whether lifestyle habits differ depending on the number of working years in rotating shift workers. <strong>Methods:</strong> We collected participant’s data from the manufacturing companies in Japan during the period from March to May in 2017. We conducted a self-administered questionnaire survey in 1561 male rotating night shift workers. Male climacteric symptoms were evaluated by using the Aging Males’ Symptoms (AMS) rating scale. We analyzed 636 rotating shift workers aged over 40 years old whose all AMS data was collected. <strong>Results:</strong> A significant difference in psychological AMS score was found between men who had worked for 10 - 19 years (9.4 ± 3.9) and men who had worked for more than 40 years (7.6 ± 3.0) (p = 0.011). Sexual functional AMS score significantly differed depending on the number of working years (less than 10 years: 8.1 ± 3.9, 10 - 19 years: 9.8 ± 4.1, 20 - 29 years: 7.9 ± 3.3, 30 - 39 years: 9.3 ± 3.5, more than 40 years: 9.3 ± 3.5) (p < 0.001). The proportion of workers with a balanced meal intake was significantly lower in men who had worked for less than 10 years and the proportions of such workers were significantly higher in men who had worked for 10 - 19 years and for more than 40 years. The proportions of men who did not drink alcohol were high in men who had worked for less than 10 years and 20 - 29 years and low in men who had worked more than 30 years. <strong>Conclusion: </strong>Psychological AMS score and sexual functional AMS score are significantly different among the 5 groups according to the number of working years on rotating shift. In addition, well-balanced diet on day shift and reduction of alcohol drinking is a related-factor for long-term rotating night shift workers.
文摘Studies on long-term wage levels of rural migrant workers are rarely found in relevant literature. On the basis of systematic collection of statistical data, this paper gives a quantitative estimation of the wage level of rural migrant workers in China and its evolution following pro market reforms since 1978. Results indicate that over the past 30 years, the nominal monetary wage of migrant workers maintained a 10% average annual increase. Real wage levels adjusted by the consumer price index (CPI) experienced three stages of evolution: initially, migrant workers earned a higher average monetary salary than formal employees, whereas this situation reversed in later stages.
文摘At present time, there has been a demand for management systems that can survey and monitor a PC (personal computer) practice room, movement of people in an office, situation of utilization of facilities and so on in real time without causing psychological stress. For example, PC administrators in Japanese national educational institutions must report operation status of PC practice rooms once a year. But, there is currently no system for automatically recording PC operating situations. Therefore, the burden on the PC administrators is big. In this study, we aimed at systems for accurately managing the sitting and work time without psychologically stressing PC users. This time, we propose uniform management systems of sitting and work time using smart tap node and mat sensor node by IoT (Internet of Things) technology. The smart tap was connected to PCs to acquire the operating status of the PCs. In parallel with this smart tap, the mat sensor was used to acquire human presence state. By calculating binary data representing the PC operating status and human presence state from the two sensing data (smart tap and mat sensor) by the proposed technique, we can exactly calculate wasteful power consumption etc. The use of IoT technology makes it unnecessary to use large installation services when introducing our system. Therefore, this our proposal system can be easily installed even by unskilled workers.
文摘Amy Tan, a Chinese American writer describes the stories between four pairs of mothers and daughters in The Joy Luck Club. Four mothers have different growing backgrounds as their daughters. This essay will analyze the reasons behind the mother-daughter's conflicts from the perspectives of the differences of eastern and western culture, mothers' educational methods and excessively high expectation of the mothers.
文摘The toxicity of benzene is well known, and its leukemia effect has established. It is a natural constituent of crude oil and the diseases related to its exposure are recognized as occupational diseases. <strong>Objective:</strong> To assess occupational exposure of benzene to workers in an oil and gas production company. <strong>Materials and Methods:</strong> Firstly, it was a descriptive, cross-sectional study which consisted of benzene atmospheric quantification in a sample individual measurement of a homogeneous exposure group of workers. Secondly, urinary assays of S-phenylmercapturic acid have been performed at the end of the shift in the selected workers.<strong> Results:</strong> The study has included 79 (47.88%) workers, 17 atmospheric samples were usable and 79 urinary assays at the end of the shift were performed. The average benzene concentration for all sites was 10 times lower than the regulatory average exposure value (1 ppm = 3.2 mg/m<sup>3</sup>): average: 0.122 pp, median: 0.053 ppm and range: 0.019 - 1.448 ppm. All 79 urinary assays of S-phenylmercapturic acid with a biological exposure index of less than 25 μg/g creatinine: mean: 0.70 μg/g creatinine, median: 0.52 μg/g creatinine and extends: 0.23 to 6.7 μg/g creatinine. <strong>Conclusion:</strong> Exposure was below to the limit value for benzene in both atmospheric metrology and biometrology. Therefore it is an occupational group with low exposure to benzene. Thus, the medical supervision will be adapted according to the potentially exposing tasks.
文摘Various networks exist in the world today including biological, social, information, and communication networks with the Internet as the largest network of all. One salient structural feature of these networks is the formation of groups or communities of vertices that tend to be more connected to each other within the same group than to those outside. Therefore, the detection of these communities is a topic of great interest and importance in many applications and different algorithms including label propagation have been developed for such purpose. Speaker-listener label propagation algorithm (SLPA) enjoys almost linear time complexity, so desirable in dealing with large networks. As an extension of SLPA, this study presented a novel weighted label propagation algorithm (WLPA), which was tested on four real world social networks with known community structures including the famous Zachary's karate club network. Wilcoxon tests on the communities found in the karate club network by WLPA demonstrated an improved statistical significance over SLPA. Withthehelp of Wilcoxon tests again, we were able to determine the best possible formation of two communities in this network relative to the ground truth partition, which could be used as a new benchmark for assessing community detection algorithms. Finally WLPA predicted better communities than SLPA in two of the three additional real social networks, when compared to the ground truth.
文摘The protein composition of the egg development in the high royal jelly producing bees (Apis mellifera L.) was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal jelly producing worker bees (Apis mellifera L.) by using two-dimensional gel electrophoresis along with their three-day development. The results showed that 160, 195, and 176 proteins, with a wide range of molecular weight (17-80 KDa) and relatively narrow scope of pI (4. 00-8.40) could be detected on day 1, day 2, and day 3, respectively, during the developmental process of the egg. Meanwhile 44 protein spots were constantly detected along with the egg development. Among them 36% were in the uptrend along with the egg development, 14% were in the downtrend, and 39% were of the largest expressed volume on day 2. In addition, the specific proteins were expressed on day 1, day 2, and day 3 (89, 77, and 80, respectively). Besides the coexistent and specific proteins, 24 proteins were expressed on day 1 and day 2, but silenced on day 3, 49 proteins were expressed on day 2 and day 3, but silenced on day 1, only 3 proteins were expressed on day 1 and day 3, but silenced on day 2. The result indicates that egg development is a sequential and complex gene controlled process, where the eggs of day 2 express the most active proteins. The coexistent proteins suggest that it is conservative and indispensable for this event. These specific proteins suggest that the different developmental stage needs specific proteins to regulate it.