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Habits on Social Networks at Workplace: A Survey of Motivations and Behaviour
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作者 Thomas Kakou Kouassi Douatia Koné +3 位作者 Aliou Bamba Aladji Kamagaté Olivier Asseu Yvon Kermarrec 《Open Journal of Applied Sciences》 2024年第8期2154-2168,共15页
This article explores the use of social networks by workers in Abidjan, Côte d’Ivoire, with particular emphasis on a descriptive or quantitative analysis aimed at understanding motivations and methods of use. Mo... This article explores the use of social networks by workers in Abidjan, Côte d’Ivoire, with particular emphasis on a descriptive or quantitative analysis aimed at understanding motivations and methods of use. More than five hundred and fifty questionnaires were distributed, highlighting workers’ preferred digital channels and platforms. The results indicate that the majority use social media through their mobile phones, with WhatsApp being the most popular app, followed by Facebook and LinkedIn. The study reveals that workers use social media for entertainment purposes and to develop professional and social relationships, with 55% unable to live without social media at work for recreational activities. In addition, 35% spend on average 1 to 2 hours on social networks, mainly between 12 p.m. and 2 p.m. It also appears that 46% believe that social networks moderately improve their productivity. These findings can guide marketing strategies, training, technology development and government policies related to the use of social media in the workplace. 展开更多
关键词 social Network social Media Applications Poisson’s Law sTATIsTICs Digital supports workers Productivity
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The Research of Cenozoic Migrant Worker Satisfaction
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作者 于海霞 《科技信息》 2010年第27期I0201-I0202,共2页
The phenomenon of Cenozoic migrant worker shortage is a realism problem of China's enterprise, and Cenozoic migrant worker has become an important force occupied the social structure. So the study of Cenozoic migr... The phenomenon of Cenozoic migrant worker shortage is a realism problem of China's enterprise, and Cenozoic migrant worker has become an important force occupied the social structure. So the study of Cenozoic migrant worker's status and the demand is imperative. Understand employees' both psychological and physiological satisfaction of enterprises' environmental factors, and make some corresponding changes is of great help to reduce the employee turnover rate. This paper commenced to research from the Cenozoic migrant worker 's characteristics, analyzed conditions and puts forward the corresponding countermeasures. 展开更多
关键词 人力资源管理 人才学 市场学 市场销售
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S-Club机制在网格信息服务系统中的实现
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作者 夏先波 富公为 刘旭东 《计算机工程》 CAS CSCD 北大核心 2007年第6期164-166,共3页
介绍了S-Club原理,分析了已有实现方法的不足,提出一个适合在网格信息服务系统中实现S-Club机制的设计方案,包括如何创建、维护和撤销S-Club以及如何在S-Club中进行查询。利用该套设计方案,在CROWN信息服务系统RLDS中成功实现了S-Club机... 介绍了S-Club原理,分析了已有实现方法的不足,提出一个适合在网格信息服务系统中实现S-Club机制的设计方案,包括如何创建、维护和撤销S-Club以及如何在S-Club中进行查询。利用该套设计方案,在CROWN信息服务系统RLDS中成功实现了S-Club机制,通过实验证明了S-Club机制确实能够提高信息服务系统的查询效率,减少综合网络开销。 展开更多
关键词 网格 信息服务 科学活动环境综合试验平台 RLDs s-club
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Association of TNF-α-238G/A and 308 G/A Gene Polymorphisms with Pulmonary Tuberculosis among Patients with Coal Worker’s Pneumoconiosis 被引量:12
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作者 HONG-MIN FAN ZHUO WANG +7 位作者 FU-MIN FENG KONG-LAI ZHANG JU-XIANG YUAN HONG SUI HONG-YAN QIU LI-HUA LIU XIAO-JUAN DENG JING-XUE REN 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2010年第2期137-145,共9页
Objectives Tumor necrosis factor-α (TNF-α) may play an important role in host's immune response to mycobacterium tuberculosis (M. tuberculosis) infection. This study was to investigate the association of TNF-α... Objectives Tumor necrosis factor-α (TNF-α) may play an important role in host's immune response to mycobacterium tuberculosis (M. tuberculosis) infection. This study was to investigate the association of TNF-α gene polymorphism with pulmonary tuberculosis (TB) among patients with coal worker's pneumoconiosis (CWP). Methods A case-control study was conducted in 113 patients with confirmed CWP complicated with pulmonary TB and 113 non-TB controls with CWP. They were matched in gender, age, job, and stage of pneumoconiosis. All participants were interviewed with questionnaires and their blood specimens were collected for genetic determination with informed consent. The TNF-α gene polymorphism was determined with polymerase chain reaction of restriction fragment length polymorphism (PCR-RFLP). Frequency of genotypes was assessed for Hardy-Weinberg equilibrium by chi-square test or Fisher's exact probability. Factors influencing the association of individual susceptibility with pulmonary TB were evaluated with logistic regression analysis. Gene-environment interaction was evaluated by a multiplieative model with combined OR. All data were analyzed using SAS version 8.2 software. Results No significant difference in frequency of the TNF-α-308 genotype was found between CWP complicated with pulmonary TB and non-TB controls (2,2=5.44, P=-0.07). But difference in frequency of the TNF-α-308 A allele was identified between them (2,2-5.14, P=0.02). No significant difference in frequencies of the TNF-α-238 genotype and allele (P=0.23 and P=0.09, respectively) was found between cases and controls either, with combined (GG and AA) OR of 3.96 (95% confidence interval of 1.30-12.09) at the -308 locus of the TNF-α gene, as compared to combination of the TNF-α-238 GG and TNF-α-308 GG genotypes. Multivariate-adjusted odds ratio of the TNF-α-238 GG and TNF-α-308 GA genotypes was 1.98 (95% CI of 1.06-3.71) for risk for pulmonary TB in patients with CWP. There was a synergic interaction between the TNF-a-308 GG genotype and body mass index (OR=4.92), as well as an interaction between the TNF-α-308 GG genotype and history of BCG immunization or history of TB exposure. And, the interaction of the TNF-α-238 GG genotype and history of BCG immunization or TB exposure with risk for pulmonary TB in them was also indicated. Conclusions TNF-α-308 A allele is associated with an elevated risk for pulmonary TB, whereas TNF-α-238 A allele was otherwise. 展开更多
关键词 Coal workers pneumoeoniosis (CWP) Pulmonary tuberculosis (TB) susceptibility POLYMORPHIsM Tumor necrosis factor (TNF) α-308 α-238 Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) Interaction
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An analysis of contributing mining factors in coal workers’pneumoconiosis prevalence in the United States coal mines,1986-2018 被引量:1
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作者 Younes Shekarian Elham Rahimi +2 位作者 Naser Shekarian Mohammad Rezaee Pedram Roghanchi 《International Journal of Coal Science & Technology》 EI CAS CSCD 2021年第6期1227-1237,共11页
In the United States,an unexpected and severe increase in coal miners’lung diseases in the late 1990s prompted researchers to investigate the causes of the disease resurgence.This study aims to scrutinize the effects... In the United States,an unexpected and severe increase in coal miners’lung diseases in the late 1990s prompted researchers to investigate the causes of the disease resurgence.This study aims to scrutinize the effects of various mining parameters,including coal rank,mine size,mine operation type,coal seam height,and geographical location on the prevalence of coal worker’s pneumoconiosis(CWP)in surface and underground coal mines.A comprehensive dataset was created using the U.S.Mine Safety and Health Administration(MSHA)Employment and Accident/Injury databases.The information was merged based on the mine ID by utilizing SQL data management software.A total number of 123,589 mine-year observations were included in the statistical analysis.Generalized Estimating Equation(GEE)model was used to conduct a statistical analysis on a total of 29,707,and 32,643 mine-year observations for underground and surface coal mines,respectively.The results of the econometrics approach revealed that coal workers in underground coal mines are at a greater risk of CWP comparing to those of surface coal operations.Furthermore,underground coal mines in the Appalachia and Interior regions are at a higher risk of CWP prevalence than the Western region.Surface coal mines in the Appalachian coal region are more likely to CWP development than miners in the Western region.The analysis also indicated that coal workers working in smaller mines are more vulnerable to CWP than those in large mine sizes.Furthermore,coal workers in thin-seam underground mine operations are more likely to develop CWP. 展开更多
关键词 Respirable coal mine dust Respiratory diseases Coal worker’s pneumoconiosis(CWP) Occupational exposure
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Differences in Male Climacteric Symptoms and Lifestyle Depending on the Duration of Working among Rotating Night Shift Workers
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作者 Sachiko Kubo Toshiyuki Yasui +2 位作者 Reiko Okahisa Yukie Matsuura Masahito Tomotake 《Health》 2020年第12期1598-1609,共12页
<strong>Background:</strong> There are various health problems in rotating shift workers. There have been few studies on the associations of male climacteric symptoms with duration of working and lifestyle... <strong>Background:</strong> There are various health problems in rotating shift workers. There have been few studies on the associations of male climacteric symptoms with duration of working and lifestyle. <strong>Objective: </strong>The aim of this study was to determine the differences in male climacteric symptoms depending on the number of working years and to determine whether lifestyle habits differ depending on the number of working years in rotating shift workers. <strong>Methods:</strong> We collected participant’s data from the manufacturing companies in Japan during the period from March to May in 2017. We conducted a self-administered questionnaire survey in 1561 male rotating night shift workers. Male climacteric symptoms were evaluated by using the Aging Males’ Symptoms (AMS) rating scale. We analyzed 636 rotating shift workers aged over 40 years old whose all AMS data was collected. <strong>Results:</strong> A significant difference in psychological AMS score was found between men who had worked for 10 - 19 years (9.4 ± 3.9) and men who had worked for more than 40 years (7.6 ± 3.0) (p = 0.011). Sexual functional AMS score significantly differed depending on the number of working years (less than 10 years: 8.1 ± 3.9, 10 - 19 years: 9.8 ± 4.1, 20 - 29 years: 7.9 ± 3.3, 30 - 39 years: 9.3 ± 3.5, more than 40 years: 9.3 ± 3.5) (p < 0.001). The proportion of workers with a balanced meal intake was significantly lower in men who had worked for less than 10 years and the proportions of such workers were significantly higher in men who had worked for 10 - 19 years and for more than 40 years. The proportions of men who did not drink alcohol were high in men who had worked for less than 10 years and 20 - 29 years and low in men who had worked more than 30 years. <strong>Conclusion: </strong>Psychological AMS score and sexual functional AMS score are significantly different among the 5 groups according to the number of working years on rotating shift. In addition, well-balanced diet on day shift and reduction of alcohol drinking is a related-factor for long-term rotating night shift workers. 展开更多
关键词 Rotating Night shift workers Male Climacteric symptoms Aging Male’s symptoms Rating scale Occupational Health Lifestyle Habit
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Wage Levels of Rural Migrant Workers in China during 1979-2010: Estimates and Trends
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作者 Lu Feng 《China Economist》 2013年第1期4-22,共19页
Studies on long-term wage levels of rural migrant workers are rarely found in relevant literature. On the basis of systematic collection of statistical data, this paper gives a quantitative estimation of the wage leve... Studies on long-term wage levels of rural migrant workers are rarely found in relevant literature. On the basis of systematic collection of statistical data, this paper gives a quantitative estimation of the wage level of rural migrant workers in China and its evolution following pro market reforms since 1978. Results indicate that over the past 30 years, the nominal monetary wage of migrant workers maintained a 10% average annual increase. Real wage levels adjusted by the consumer price index (CPI) experienced three stages of evolution: initially, migrant workers earned a higher average monetary salary than formal employees, whereas this situation reversed in later stages. 展开更多
关键词 wage of rural migrant workers migration of labor force China's labormarket
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本期热播:英国偶像组合S Club:《你是否曾经》
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作者 Thea Peddie 林霖 《疯狂英语(初中天地)》 北大核心 2005年第7期-,共2页
英国乐队组合S Club是90年代后期的青少年流行音乐复苏所付出的最大贡献的其中一员。S Club最初叫S Club 7(七小龙),由辣妹前经纪人西门·富勒组建。乐队的成员是从来自欧洲各地的1万名候选人中挑选出来的。当然。
关键词 s club
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Development of PC Worker’s Management System in PC Training Room and Office by Using IoT Technology
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作者 Keiichi Abe Yuki Takabayashi Masao Isshiki 《Journal of Electrical Engineering》 2018年第4期221-228,共8页
At present time, there has been a demand for management systems that can survey and monitor a PC (personal computer) practice room, movement of people in an office, situation of utilization of facilities and so on i... At present time, there has been a demand for management systems that can survey and monitor a PC (personal computer) practice room, movement of people in an office, situation of utilization of facilities and so on in real time without causing psychological stress. For example, PC administrators in Japanese national educational institutions must report operation status of PC practice rooms once a year. But, there is currently no system for automatically recording PC operating situations. Therefore, the burden on the PC administrators is big. In this study, we aimed at systems for accurately managing the sitting and work time without psychologically stressing PC users. This time, we propose uniform management systems of sitting and work time using smart tap node and mat sensor node by IoT (Internet of Things) technology. The smart tap was connected to PCs to acquire the operating status of the PCs. In parallel with this smart tap, the mat sensor was used to acquire human presence state. By calculating binary data representing the PC operating status and human presence state from the two sensing data (smart tap and mat sensor) by the proposed technique, we can exactly calculate wasteful power consumption etc. The use of IoT technology makes it unnecessary to use large installation services when introducing our system. Therefore, this our proposal system can be easily installed even by unskilled workers. 展开更多
关键词 PC worker’s management system smart tap node mat sensor node IoT technology sensor network.
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On Mother-Daughter's Conflicts in The Joy Luck Club
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作者 郑清颖 《海外英语》 2019年第6期232-233,共2页
Amy Tan, a Chinese American writer describes the stories between four pairs of mothers and daughters in The Joy Luck Club. Four mothers have different growing backgrounds as their daughters. This essay will analyze th... Amy Tan, a Chinese American writer describes the stories between four pairs of mothers and daughters in The Joy Luck Club. Four mothers have different growing backgrounds as their daughters. This essay will analyze the reasons behind the mother-daughter's conflicts from the perspectives of the differences of eastern and western culture, mothers' educational methods and excessively high expectation of the mothers. 展开更多
关键词 mother-daughter’s conflicts IMMIGRANTs The JOY LUCK club
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Club 3D正式推出首款S3 Deltachrome显卡
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《电击高手》 2004年第2期8-8,共1页
关键词 s3 DELTACHROME 显卡 club 3D 输出接口 显存
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云南网络英语4S+club体验馆模式的探究
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作者 贺永进 《经贸实践》 2017年第4X期288-288,共1页
随着云南经济的不断发展,英语学习的重要性也逐渐显现出来,本文中提出云南网络英语4S+club体验馆模式,并对其模式的优势进行详细分析。
关键词 网络英语 4s%PLUs%club
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Benzene Monitoring and S-Phenylmercapturic Acid Determination of Workers at Oil Sites in Congo-Brazzaville
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作者 Ebenguela Ataboho Ebatetou Josué Richard Ntsimba Nsemi Donatien Moukassa 《Occupational Diseases and Environmental Medicine》 2021年第3期127-138,共12页
The toxicity of benzene is well known, and its leukemia effect has established. It is a natural constituent of crude oil and the diseases related to its exposure are recognized as occupational diseases. <strong>... The toxicity of benzene is well known, and its leukemia effect has established. It is a natural constituent of crude oil and the diseases related to its exposure are recognized as occupational diseases. <strong>Objective:</strong> To assess occupational exposure of benzene to workers in an oil and gas production company. <strong>Materials and Methods:</strong> Firstly, it was a descriptive, cross-sectional study which consisted of benzene atmospheric quantification in a sample individual measurement of a homogeneous exposure group of workers. Secondly, urinary assays of S-phenylmercapturic acid have been performed at the end of the shift in the selected workers.<strong> Results:</strong> The study has included 79 (47.88%) workers, 17 atmospheric samples were usable and 79 urinary assays at the end of the shift were performed. The average benzene concentration for all sites was 10 times lower than the regulatory average exposure value (1 ppm = 3.2 mg/m<sup>3</sup>): average: 0.122 pp, median: 0.053 ppm and range: 0.019 - 1.448 ppm. All 79 urinary assays of S-phenylmercapturic acid with a biological exposure index of less than 25 μg/g creatinine: mean: 0.70 μg/g creatinine, median: 0.52 μg/g creatinine and extends: 0.23 to 6.7 μg/g creatinine. <strong>Conclusion:</strong> Exposure was below to the limit value for benzene in both atmospheric metrology and biometrology. Therefore it is an occupational group with low exposure to benzene. Thus, the medical supervision will be adapted according to the potentially exposing tasks. 展开更多
关键词 BENZENE METROLOGY s-Phenylmercapturic Acid Petroleum site workers
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Chinese Trade Union's Efforts on Protection of Women Workers'Rights and Interests
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《International Understanding》 2002年第3期28-32,共5页
关键词 Chinese Trade Union’s Efforts on Protection of Women workers’Rights and Interests
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焦炉工血清GST活力与尿1-羟基芘和GSTM1、GSTT1基因多态的关系 被引量:4
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作者 刘爱林 南培宏 +3 位作者 孙建娅 田建峰 张弛 鲁文清 《环境与健康杂志》 CAS CSCD 北大核心 2006年第2期109-111,共3页
目的研究血清谷胱甘肽硫转移酶(GST)能否作为焦炉工人多环芳烃的暴露评价指标,同时探讨GSTM1、GSTT1基因多态性与血清GST活力的关系。方法选择某焦化厂39名男性焦炉工人和44名男性对照工人,采用统一的调查表调查一般情况。采集静脉血分... 目的研究血清谷胱甘肽硫转移酶(GST)能否作为焦炉工人多环芳烃的暴露评价指标,同时探讨GSTM1、GSTT1基因多态性与血清GST活力的关系。方法选择某焦化厂39名男性焦炉工人和44名男性对照工人,采用统一的调查表调查一般情况。采集静脉血分析血清GST活力和GSTM1、GSTT1基因型,采集尿样分析尿1-羟基芘(1-OHP)浓度。血清GST活力用试剂盒检测;尿1-羟基芘(1-OHP)浓度作为多环芳烃内暴露剂量,用碱水解-高效液相色谱方法测定;GSTM1和GSTT1基因多态性分析采用PCR方法。结果焦炉工人尿1-OHP浓度(中位数为5.60μmol/mol肌酐)和血清GST活力(中位数25.75U/ml)分别高于对照组(尿1-OHP浓度:中位数1.32μmol/mol肌酐,血清GST活力:14.54U/ml),差异均有统计学意义(均P<0.001)。GSTM1(-/-)和GSTT1(-/-)型工人的血清GST活力分别与GSTM1(-/+,+/+)和GSTT1(-/+,+/+)型工人的血清GST活力相比,差异均无统计学意义(均P>0.05)。广义线性回归分析显示,尿1-OHP对血清GST的影响具有统计学意义(P<0.01)。血清GST活力和尿1-OHP浓度存在统计学正相关(r=0.357,P<0.001,n=83)。结论焦炉工人血清GST有可能作为多环芳烃暴露评价的参数;未见GSTM1和GSTT1基因缺失对血清GST活力的影响。 展开更多
关键词 基因 谷胱甘肽硫转移酶 1-羟基芘 焦炉工
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基于D-S证据理论的地下洞室作业人员安全行为评价 被引量:8
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作者 郑霞忠 石法起 +2 位作者 陈述 刘晶 吴黎明 《中国安全生产科学技术》 CAS CSCD 北大核心 2016年第6期158-162,共5页
为系统地评价地下洞室作业人员安全行为水平,综合运用集对分析和D-S证据理论,提出一种基于D-S证据理论的作业人员安全行为评价方法。首先,分析了影响作业人员安全行为的因素,构建包括作业人员安全素养、生理因素、心理因素、作业技术等... 为系统地评价地下洞室作业人员安全行为水平,综合运用集对分析和D-S证据理论,提出一种基于D-S证据理论的作业人员安全行为评价方法。首先,分析了影响作业人员安全行为的因素,构建包括作业人员安全素养、生理因素、心理因素、作业技术等4个一级指标及16个二级指标的综合评价指标体系,并将安全行为等级划分为4级;其次,采用集对分析处理二级指标的不确定性,产生基本信度分配函数(BPA);然后,利用D-S证据理论,融合一级指标的BPA,产生最终安全等级隶属度;最后,结合最大隶属度原则和信任准则,确定地下洞室作业人员安全行为等级。以某水电站引水隧洞开挖工程为例,确定该工程中作业人员行为安全等级为z2级,即一般安全,符合工程实际。 展开更多
关键词 安全行为 地下洞室 D-s证据理论 集对分析 作业人员
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S型曲线在我国体育预测问题中的应用研究 被引量:2
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作者 魏华 《电子设计工程》 2011年第16期61-63,共3页
根据全国2002~2009年全国俱乐部数据,比较直线趋势模型、二次曲线模型、三次曲线模型、对数曲线模型和S曲线模型在俱乐部发展数据预测拟合效果的优劣,为相关研究提供合适的预测模型。应用5种预测方法:对全国俱乐部总数、未来5年的总数... 根据全国2002~2009年全国俱乐部数据,比较直线趋势模型、二次曲线模型、三次曲线模型、对数曲线模型和S曲线模型在俱乐部发展数据预测拟合效果的优劣,为相关研究提供合适的预测模型。应用5种预测方法:对全国俱乐部总数、未来5年的总数预测并比较拟合值的绝对误差、平均相对误差绝对值、误差平方和。对全国健身俱乐部发展数量方面,S预测模型相对误差绝对值最小。S预测模型对全国健身俱乐部发展规模变化规律的分析有比较好的适应性和实用性,可以相关管理部门制定的发展政策提供了一定的理论依据,为《全民健身条例》的落实提供科学依据。 展开更多
关键词 s型曲线 预测模型 健身俱乐部总数 发展规模
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萧劲光领导安源路矿工人游艺工作考
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作者 张辉海 池康燕 《萍乡学院学报》 2024年第2期15-19,共5页
萧劲光是我国杰出的无产阶级革命家、军事家,国家和军队的优秀领导人,人民海军的主要创建者。萧劲光1924年从苏联留学回国后,10月初奉派到安源工作,1925年7月下旬离开安源奔赴广东参加国民革命。安源是萧劲光革命生涯的首站,在安源,萧... 萧劲光是我国杰出的无产阶级革命家、军事家,国家和军队的优秀领导人,人民海军的主要创建者。萧劲光1924年从苏联留学回国后,10月初奉派到安源工作,1925年7月下旬离开安源奔赴广东参加国民革命。安源是萧劲光革命生涯的首站,在安源,萧劲光一直担任安源路矿工人俱乐部游艺执行委员兼游艺委员会委员长,是安源路矿工人运动的领导人之一,负责领导游艺部门,工作卓有成效,为安源路矿工人运动作出了重要贡献。 展开更多
关键词 萧劲光 安源路矿工人俱乐部 游艺委员会 游艺工作 执行委员
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Finding Statistically Significant Communities in Networks with Weighted Label Propagation 被引量:2
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作者 Wei Hu 《Social Networking》 2013年第3期138-146,共9页
Various networks exist in the world today including biological, social, information, and communication networks with the Internet as the largest network of all. One salient structural feature of these networks is the ... Various networks exist in the world today including biological, social, information, and communication networks with the Internet as the largest network of all. One salient structural feature of these networks is the formation of groups or communities of vertices that tend to be more connected to each other within the same group than to those outside. Therefore, the detection of these communities is a topic of great interest and importance in many applications and different algorithms including label propagation have been developed for such purpose. Speaker-listener label propagation algorithm (SLPA) enjoys almost linear time complexity, so desirable in dealing with large networks. As an extension of SLPA, this study presented a novel weighted label propagation algorithm (WLPA), which was tested on four real world social networks with known community structures including the famous Zachary's karate club network. Wilcoxon tests on the communities found in the karate club network by WLPA demonstrated an improved statistical significance over SLPA. Withthehelp of Wilcoxon tests again, we were able to determine the best possible formation of two communities in this network relative to the ground truth partition, which could be used as a new benchmark for assessing community detection algorithms. Finally WLPA predicted better communities than SLPA in two of the three additional real social networks, when compared to the ground truth. 展开更多
关键词 Community Detection social NETWORKs WEIGHTED LABEL Propagation statistical significance Zachary’s KARATE club Network
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Profile Analysis of the Proteome of the Egg of the High Royal Jelly Producing Bees (Apis mellifera L.) 被引量:1
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作者 ZHANG Ean LI Jian-ke WU Li-ming 《Agricultural Sciences in China》 CAS CSCD 2007年第9期1138-1148,共11页
The protein composition of the egg development in the high royal jelly producing bees (Apis mellifera L.) was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal j... The protein composition of the egg development in the high royal jelly producing bees (Apis mellifera L.) was investigated. This pioneer study was to separate and quantify the proteins in the egg of the high royal jelly producing worker bees (Apis mellifera L.) by using two-dimensional gel electrophoresis along with their three-day development. The results showed that 160, 195, and 176 proteins, with a wide range of molecular weight (17-80 KDa) and relatively narrow scope of pI (4. 00-8.40) could be detected on day 1, day 2, and day 3, respectively, during the developmental process of the egg. Meanwhile 44 protein spots were constantly detected along with the egg development. Among them 36% were in the uptrend along with the egg development, 14% were in the downtrend, and 39% were of the largest expressed volume on day 2. In addition, the specific proteins were expressed on day 1, day 2, and day 3 (89, 77, and 80, respectively). Besides the coexistent and specific proteins, 24 proteins were expressed on day 1 and day 2, but silenced on day 3, 49 proteins were expressed on day 2 and day 3, but silenced on day 1, only 3 proteins were expressed on day 1 and day 3, but silenced on day 2. The result indicates that egg development is a sequential and complex gene controlled process, where the eggs of day 2 express the most active proteins. The coexistent proteins suggest that it is conservative and indispensable for this event. These specific proteins suggest that the different developmental stage needs specific proteins to regulate it. 展开更多
关键词 HONEYBEEs high royal jelly producing worker bee's egg two-dimensional gel electrophoresis PROTEOME
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