WRAP53与甲状腺乳头状癌患者临床病理特征及预后的关系

目的:探讨端粒酶新核心亚单位(WD40-encoding RNA antisense to p53,WRAP53)又名端粒酶卡哈尔体蛋白1(telomerase cajal body protein 1,TCAB1)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)组织中的表达,分析其与患者临床病理特征及预后的关系。方法:收集28例PTC患者的癌及癌周正常新鲜组织标本,应用实时荧光定量聚合酶链反应法(quantitative real-time polymerase chain reaction,qPR-PCR)及免疫组织化学法分别检测WRAP53 mRNA及蛋白在PTC及周围正常组织中的表达,收集117例PTC术后患者的临床数据及对应的癌组织的石蜡切片进行免疫组化检测,分析其蛋白表达水平与甲状腺乳头状癌患者的临床病理特征的相关性,最后应用蛋白质表达图谱(The Human Protein Atlas,HPA)数据库探究WRAP53与PTC预后之间的关系。结果:qPR-PCR及免疫组化结果提示WRAP53在PTC中较正常组织高表达(P<0.05)。与WRAP53低表达的PTC患者相比,WRAP53高表达的PTC患者肿瘤直径更长,更易出现淋巴结转移(P<0.05)。HPA数据库显示WRAP53高表达的PTC患者的生存率差于WRAP53低表达的PTC患者(P=0.041)。结论:通过本次研究表明,WRAP53在PTC组织中表达较周围正常组织升高,且其高表达与PTC增殖、淋巴结转移及不良预后相关。WRAP53可能是治疗PTC新的潜在靶点。

A functional tacrolimus-releasing nerve wrap for enhancing nerve regeneration following surgical nerve repair

Axonal regeneration following surgical nerve repair is slow and often incomplete,resulting in poor functional recovery which sometimes contributes to lifelong disability.Currently,there are no FDA-approved therapies available to promote nerve regeneration.Tacrolimus accelerates axonal regeneration,but systemic side effects presently outweigh its potential benefits for peripheral nerve surgery.The authors describe herein a biodegradable polyurethane-based drug delivery system for the sustained local release of tacrolimus at the nerve repair site,with suitable properties for scalable production and clinical application,aiming to promote nerve regeneration and functional recovery with minimal systemic drug exposure.Tacrolimus is encapsulated into co-axially electrospun polycarbonate-urethane nanofibers to generate an implantable nerve wrap that releases therapeutic doses of bioactive tacrolimus over 31 days.Size and drug loading are adjustable for applications in small and large caliber nerves,and the wrap degrades within 120 days into biocompatible byproducts.Tacrolimus released from the nerve wrap promotes axon elongation in vitro and accelerates nerve regeneration and functional recovery in preclinical nerve repair models while off-target systemic drug exposure is reduced by 80%compared with systemic delivery.Given its surgical suitability and preclinical efficacy and safety,this system may provide a readily translatable approach to support axonal regeneration and recovery in patients undergoing nerve surgery.

Blade Wrap Angle Impact on Centrifugal Pump Performance:Entropy Generation and Fluid-Structure Interaction Analysis

The centrifugal pump is a prevalent power equipment widely used in different engineering patterns,and the impeller blade wrap angle significantly impacts its performance.A numerical investigation was conducted to analyze the influence of the blade wrap angle on flow characteristics and energy distribution of a centrifugal pump evaluated as a low specific speed with a value of 69.This study investigates six impellermodels that possess varying blade wrap angles(95°,105°,115°,125°,135°,and 145°)that were created while maintaining the same volute and other geometrical characteristics.The investigation of energy loss was conducted to evaluate the values of total and entropy generation rates(TEG,EGR).The fluid-structure interaction was considered numerically using the software tools ANSYS Fluent and ANSYSWorkbench.The elastic structural dynamic equation was used to estimate the structural response,while the shear stress transport k–ωturbulence model was utilized for the fluid domain modeling.The findings suggest that the blade wrap angle has a significant influence on the efficiency of the pump.The impeller featuring a blade wrap angle of 145°exhibits higher efficiency,with a notable increase of 3.76%relative to the original model.Variations in the blade wrap angle impact the energy loss,shaft power,and pump head.The model with a 145°angle exhibited a maximum equivalent stress of 14.8MPa and a total deformation of 0.084 mm.The results provide valuable insights into the intricate flow mechanism of the centrifugal pump,particularly when considering various blade wrap angles.

基于Geomagic Wrap软件对铣刀盘的点云处理

Geomagic Wrap软件在逆向工程中经常用于对三维扫描的点云数据进行处理,通过铣刀盘零件的点云处理流程,分析Geomagic Wrap软件对于点云处理精度和有关影响因素,通过选择合理的命令为后续零件点云处理作参考。

A Wrapping Encryption Based on Double Randomness Mechanism

Currently,data security mainly relies on password(PW)or system channel key(SKCH)to encrypt data before they are sent,no matter whether in broadband networks,the 5th generation(5G)mobile communications,satellite communications,and so on.In these environments,a fixed password or channel key(e.g.,PW/SKCH)is often adopted to encrypt different data,resulting in security risks since thisPW/SKCH may be solved after hackers collect a huge amount of encrypted data.Actually,the most popularly used security mechanism Advanced Encryption Standard(AES)has its own problems,e.g.,several rounds have been solved.On the other hand,if data protected by the same PW/SKCH at different time points can derive different data encryption parameters,the system’s security level will be then greatly enhanced.Therefore,in this study,a security scheme,named Wrapping Encryption Based on Double Randomness Mechanism(WEBDR),is proposed by integrating a password key(or a system channel key)and an Initialization Vector(IV)to generate an Initial Encryption Key(IEK).Also,an Accumulated Shifting Substitution(ASS)function and a three-dimensional encryption method are adopted to produce a set of keys.Two randomness encryption mechanisms are developed.The first generates system sub-keys and calculates the length of the first pseudo-random numbers by employing IEK for providing subsequent encryption/decryption.The second produces a random encryption key and a sequence of internal feedback codes and computes the length of the second pseudo-random numbers for encrypting delivered messages.A wrapped mechanism is further utilized to pack a ciphertext file so that a wrapped ciphertext file,rather than the ciphertext,will be produced and then transmitted to its destination.The findings are as follows.Our theoretic analyses and simulations demonstrate that the security of the WEBDR in cloud communication has achieved its practical security.Also,AES requires 176 times exclusive OR(XOR)operations for both encryption and decryption,while the WEBDR consumes only 3 operations.That is why the WEBDR is 6.7∼7.09 times faster than the AES,thus more suitable for replacing the AES to protect data transmitted between a cloud system and its users.

“Capsule drape wrap”——a new technology for iridoschisis management during phacoemulsification

·AIM: To introduce a new technique for iridoschisis management during phacoemulsification: “capsule drape wrap”.·METHODS: “Capsule drape wrap” technique was used for an 80-year-old man with idiopathic iridoschisis in the right eye during phacoemulsification. The inserted flexible nylon iris hooks to hold anterior capsule in place, the margin of the anterior capsule could act as drape wrap, tracking the fibrillary iris strands firmly from free floating and stabilizing the capsular bags simultaneously. ·RESULTS: The eye with iridoschisis was successfully treated. Iris fibrils remained immobile during the procedure, and despite the severity of iridoschisis, there were no intraoperative complications such as tear of the iris, hyphema, iris prolapse, loss of mydriasis, or rupture of the posterior lens capsule during phacoemulsification. The bestcorrected visual acuity was increased by 0.1(log MAR) 6mo after the surgery.·CONCLUSION: “Capsule drape wrap” for iridoschisis is easily manageable, prevents further disruption to the loose iris fibers and ensures the stability of capsule–iris complex simultaneously, consequently minimizing the risk of surgical complications in phacoemulsification.

Research on web service wrapping for command line programs

A web service wrapping approach for command line programs,which are commonly used in scientific computing,is proposed.First,software architecture for a basic web service wrapper implementation is given and the functions of the main components are explained.Then after a comprehensive analysis of data transmission and a job life cycle model,a novel proactive file transmission and job management mechanism is devised to enhance the software architecture,and the command line programs are wrapped into web services in such a way that they can efficiently transmit files,supply instant status feedback and automatically manage the jobs.Experiments show that the proposed approach achieves higher performance with less memory usage compared to the related work, and the usability is also improved.This work has already been put into use in a production system of scientific computing and the data processing efficiency of the system is greatly improved.

WRAP水权分析模型及其应用

WRAP(Water Rights Analysis Package)水权分析模型是基于水资源优先分配制度来模拟和预测一个或多个流域内水资源使用及管理的模型。作者首次将WRAP水权分析模型应用于北京通州区,以1956～2007年通州区相关资料作为模型的计算数据,模拟与计算结果表明:通州地区的天然年径流总体趋势基本保持不变,调度径流呈微弱增长趋势,两者的年际变差均逐渐剧烈;历年的径流消耗量总体趋势亦基本不变,但回归流呈逐渐增长趋势;研究区内多年平均水量保证率为59.9%,工业用水、城镇生活用水和农村生活用水呈上升趋势,而农业用水量总体呈下降趋势。

湿地生态健康快速评价法简介——以WRAP为例

快速评价法是最早被用于湿地评价的方法,它能在较少的资金投入和劳动强度下对湿地的健康状况作出较为科学和可靠的评价,因而也是目前最为常用的湿地健康评价方法。简介了湿地快速评价方法的内涵及评价步骤,并以佛罗里达州的快速评价方法(WRAP)为例介绍了该方法在实际研究中的应用,希望能为我国湿地生态健康评价提供参考。

基于Geomagic Wrap的发动机活塞逆向设计

逆向工程是根据已经存在的产品,反向推出产品设计数据的过程。以某型号发动机活塞为例,利用三维扫描仪对其进行三维扫描,获取活塞的点云数据,再使用Geomagic Wrap软件对点云数据进行处理并生成曲面模型,最后对活塞曲面模型进行偏差分析。研究结果表明,逆向工程技术可以大幅度速断研发周期,具有高效性和可行性。

Wrapped MOOC质量评价指标体系及模型

评估与考核是接受和推进在线教育最有力的策略。利用比较分析法、层次分析法及德尔斐(Delphi)法,构建了"Wrapped MOOC"质量评价指标层次;针对运用层次分析法得到的正互反矩阵的一致性检验不合格影响排序的可靠性这一问题,提出一种改进的模糊综合评价法对正互反矩阵进行微调,以使随机一致性比率CR符合要求;计算得到各级指标的评价矩阵的特征值及Wrapped MOOC质量评价模型,并进行数值测试。研究结果表明,准则层C1的5要素单排序结果为:教学质量、课堂组织、课程资源、课前设计、学习用户。数值仿真验证了该算法及模型的有效性。

人WRAP53基因启动子区的生物信息学分析

人WRAP53基因隶属于WD重复蛋白家族,被发现在端粒酶的组装、卡哈尔体的形成和DNA损伤的修复等过程中发挥着重要作用,但WRAP53基因表达调控的分子机制尚不明确.利用生物信息学软件对人WRAP53基因近端启动子-2 000 bp～-1 bp区域的结构特征、转录因子结合位点和CpG岛进行预测分析.Promotor 2.0和NNPP均预测出人WRAP53β基因有2个启动子;JASPAR和AliBaba 2.1发现有众多转录因子潜在结合位点存在该序列上;CpG Plot、CpG Finder和MethPrimer软件均预测出人WRAP53基因启动子-2 000 bp～-1 bp区域存在1个CpG岛.对人WRAP53基因启动子区开展的生物信息学分析,有助于了解该基因启动子区的结构和功能,为后续开展人WRAP53基因表达调控机制研究奠定了基础.

基于Geomagic Wrap的甲壳虫玩具汽车车身逆向设计

通过对甲壳虫玩具汽车车身模型进行三维光学扫描获取其表面点云数据,利用逆向工程软件Geomagic Wrap进行逆向造型设计,将扫描数据进行点云数据处理、多边形和精确曲面片的编辑处理而实现快速构建甲壳虫玩具汽车车身NURBS曲面模型,并对重构的曲面模型进行偏差分析,得到实物原型的CAD模型,为相似模型的建模与设计提供了一定的参考。

试论短片《Wrapped(毁灭)》中的生态美学思想

短片《Wrapped(毁灭)》是德国巴登-符腾堡州电影学院的3名学生合作完成的毕业设计作品。本文从生态美学的视角出发,探寻该短片所传达出的生态本位思想以及万物共生的和谐之美。生态为本才能探寻生命的力量;维持生态平衡,才能让人类与大自然和谐共生;灾难降临的假设,能够唤醒人们对于家园意识的追寻。

Influence of Blade Wrap Angle on Centrifugal Pump Performance by Numerical and Experimental Study

The existing research on improving the hydraulic performance of centrifugal pumps mainly focuses on the design method and the parameter optimization. The traditional design method for centrifugal impellers relies more on experience of engineers that typically only satisfies the continuity equation of the fluid. In this study, on the basis of the direct and inverse iteration design method which simultaneously solves the continuity and motion equations of the fluid and shapes the blade geometry by controlling the wrap angle, three centrifugal pump impellers are designed by altering blade wrap angles while keeping other parameters constant. The three-dimensional flow fields in three centrifugal pumps are numerically simulated, and the simulation results illustrate that the blade with larger wrap angle has more powerful control ability on the flow pattern in impeller. The three pumps have nearly the same pressure distributions at the small flow rate, but the pressure gradient increase in the pump with the largest wrap angle is smoother than the other two pumps at the design and large flow rates. The pump head and efficiency are also influenced by the blade wrap angle. The highest head and efficiency are also observed for the largest angle. An experiment rig is designed and built to test the performance of the pump with the largest wrap angle. The test results show that the wide space of its efficiency area and the stability of its operation ensure the excellent performance of the design method and verify the numerical analysis. The analysis on influence of the blade wrap angle for centrifugal pump performance in this paper can be beneficial to the optimization design of the centrifugal pump.

WRAP53β的分子功能及其在疾病中的作用

WRAP53β蛋白是WD40重复蛋白家族的重要成员,能控制端粒酶、DNA修复蛋白和剪接因子在细胞内转运,参与卡哈尔体形成、端粒稳定和端粒延伸。目前研究表明WRAP53β功能异常与早衰、肿瘤、神经退行性疾病等密切相关,可能成为疾病治疗的一个新靶点。现就WRAP53β在细胞内多方面的分子功能及其与疾病关系的研究进展进行综述。

基于Geomagic Wrap的自由曲面零件逆向设计

以自由曲面工艺品逆向设计为例,介绍了基于Geomagic Wrap实现点云数据处理、逆向建模的过程。结果表明,对于复杂自由曲面类零件,通过分割特征、构造有序的栅格、重构曲面片的逆向建模方法,有利于提高曲面的光顺度与精度。

“Wrapping the gastroduodenal artery stump” during pancreatoduodenectomy reduced the stump hemorrhage incidence after operation

Objective： After pancreaticoduodenectomy （PD）, the postoperative gastroduodenal artery stump （GDAS） hemorrhage is one of the most serious complications. The purpose of this study is to determine whether wrapping the GDAS during PD could decrease the postoperative GDAS hemorrhage incidence. Methods： A retrospective review involving 280 patients who underwent PD from 2005 to 2012 was performed. Wrapping the GDAS during PD was defined as ＂Wrapping the GDAS using the teres hepatis ligamentum during PD＂. A total of 140 patients accepted the ＂wrapping＂ procedure （wrapping group）. The other 140 patients didn＇t apply the procedure （non-wrapping group）. Age, sex, preoperative data, estimated intraoperative blood loss, postoperative complications, pathologic parameters and hospitalization time were compared between two groups. ResultsI There were no significant differences in patient characteristics between two groups. After wrapping, the incidence of postoperative GDAS bleeding decreased significantly （1/140 vs. 9/140, P=0.01）. The rates of the other complications （such as intra-abdominal infection pancreatic fistula, billiary fistula, gastrointestinal bleeding, et aL） showed no significant differences. Conclusions： Wrapping the GDAS during PD significantly reduced the postoperative GDAS hemorrhage incidence. And the ＂wrapping＂ had no obvious influence on other complications.

WRAP53 β调控DNA损伤修复进展

WRAP53β是一种具有WD40结构域的蛋白质,在维护卡哈尔体稳定、RNA剪接、端粒延伸等方面起着至关重要的作用.WRAP53β功能紊乱与先天性角化不良、肿瘤、进行性脊髓性肌萎缩、过早老化等疾病有关.近两年研究发现WRAP53β是DNA双链断裂修复(DSBs)的一个重要支架蛋白,它以一种依赖于ATM、H2AX、MDC1的方式被募集至损伤位点并磷酸化,其WD40结构域可募集泛素E3连接酶RNF8,将DSBs位点附近的组蛋白H2AX泛素化,促进下游修复因子的聚集,引起DNA损伤后的修复作用.为此,我们重点综述了现阶段WRAP53β在DNA损伤修复方面的具体作用及机制.