The expression vector containing phbB and ble genes was constructed and transformed into cell-wall- deficient strain Chlamydomonas reinhardtii CC-849 by the glass-bead method. The transgenic alga was selected and main...The expression vector containing phbB and ble genes was constructed and transformed into cell-wall- deficient strain Chlamydomonas reinhardtii CC-849 by the glass-bead method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mg/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.展开更多
文摘The expression vector containing phbB and ble genes was constructed and transformed into cell-wall- deficient strain Chlamydomonas reinhardtii CC-849 by the glass-bead method. The transgenic alga was selected and maintained in the TAP agar plates containing 10 mg/mL Zeomycin. Transgenic alga, which could express phbB at the transcriptional level, was obtained and further confirmed with PCR, Southern blot and RT-PCR-DNA hybridization analysis.
