OBJECTIVE:To investigate the effectiveness and safety of Jiawei Xiaoyao pill(加味逍遥丸,JXP)in the treatment of symptoms associated with premenstrual syndrome(PMS).METHODS:A total of 144 regularly menstruating women w...OBJECTIVE:To investigate the effectiveness and safety of Jiawei Xiaoyao pill(加味逍遥丸,JXP)in the treatment of symptoms associated with premenstrual syndrome(PMS).METHODS:A total of 144 regularly menstruating women with PMS were recruited at 8 sites in China from August 2017 to December 2018,and randomized to receive either a JXP or a matching placebo(12 g/d,6 g twice a day)for 3 menstrual cycles.The primary indicator was the reduced Daily Record of Severity of Problems(DRSP)scores in the luteal phase after 3 months of treatment.The safety outcomes included clinical adverse events(AEs),adverse reactions(ARs),changes in vital signs,and laboratory tests.RESULTS:JXP surpassed the placebo in reducing DRSP scores(psychological/somatic dysfunction)in the luteal phase over 3 menstrual cycles of treatment(PFAS=0.002,PPPS=0.001).Additionally,there were no significant differences in the incidence of AEs,severe AEs,withdrawal due to AEs and ARs between the two groups(all P>0.05),and no clinically significant adverse medical events related to the test drug observed.CONCLUSIONS:JXP was superior to the placebo in relieving the symptoms associated with PMS,which signified that JXP may be effective,safe,and welltolerated as an alternative therapy.展开更多
Objective： To investigate the effects of 50% ethyl alcohol （EtOH） extracts from Danzhi Xiaoyao Pill （丹栀逍遥丸, DXP） on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. Methods： AT...Objective： To investigate the effects of 50% ethyl alcohol （EtOH） extracts from Danzhi Xiaoyao Pill （丹栀逍遥丸, DXP） on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. Methods： ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity （ORAC）. The effects of DXP on nitric oxide （NO） production were tested by lipopolysaccharide （LPS）- stimulated RAW 264.7 murine macrophages using the Griess reaction. Results： The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg/mL dosedependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820μ moL Trolox equivalent/g, about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL （P〈0.05, P〈0.01）. Conclusion： The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.展开更多
目的探讨逍遥丸改善产前应激子代大鼠焦虑样行为的作用机制。方法采用随机数字表法选择28只SD子代大鼠,根据是否应激、灌胃不同药物,将大鼠分为对照组、产前应激组、产前应激+逍遥丸组和产前应激+氯化钠组,每组7只。然后通过旷场实验和...目的探讨逍遥丸改善产前应激子代大鼠焦虑样行为的作用机制。方法采用随机数字表法选择28只SD子代大鼠,根据是否应激、灌胃不同药物,将大鼠分为对照组、产前应激组、产前应激+逍遥丸组和产前应激+氯化钠组,每组7只。然后通过旷场实验和高架十字实验观察各组子代大鼠的焦虑样行为,随后提取海马组织,分别采用荧光定量聚合酶链反应(PCR)和Western blot法观察谷氨酸受体A1亚基(GluA1)信使RNA(mRNA)和蛋白表达的变化。结果在旷场实验中,与对照组相比,产前应激组潜伏期明显延长,中央区时间百分比和运动距离明显减少(P<0.01);与产前应激+氯化钠组相比,产前应激+逍遥丸组子代大鼠潜伏期明显缩短,中央区时间百分比和运动距离明显增加[(55±14)s比(108±10)s、(14.8±2.4)%比(6.6±1.8)%、(2673±296)cm比(1747±353)cm](P<0.01);但各组子代大鼠运动速度比较差异无统计学意义(P>0.05)。在高架十字迷宫实验中,与对照组相比,产前应激组子代大鼠开放臂运动距离和开放臂时间百分比明显减少(P<0.01);与产前应激+氯化钠组相比,产前应激+逍遥丸组开放臂运动距离和开放臂时间百分比明显增加[(157±20)cm比(21±3)cm、(0.74±0.14)%比(0.50±0.15)%](P<0.01);但各组子代大鼠运动速度比较差异无统计学意义(P>0.05)。荧光定量PCR和Western blot法结果显示,与对照组相比,产前应激组子代大鼠海马中GluA1 mRNA和蛋白表达显著降低(P<0.01);与产前应激+氯化钠组相比,产前应激+逍遥丸组子代大鼠海马中GluA1 mRNA和蛋白表达显著升高(0.950±0.199 vs 0.491±0.181,0.663±0.047 vs 0.464±0.074)(P<0.01)。结论逍遥丸可明显改善产前应激子代大鼠焦虑样行为,其作用机制可能与提高海马区GluA1的表达有关。展开更多
文摘OBJECTIVE:To investigate the effectiveness and safety of Jiawei Xiaoyao pill(加味逍遥丸,JXP)in the treatment of symptoms associated with premenstrual syndrome(PMS).METHODS:A total of 144 regularly menstruating women with PMS were recruited at 8 sites in China from August 2017 to December 2018,and randomized to receive either a JXP or a matching placebo(12 g/d,6 g twice a day)for 3 menstrual cycles.The primary indicator was the reduced Daily Record of Severity of Problems(DRSP)scores in the luteal phase after 3 months of treatment.The safety outcomes included clinical adverse events(AEs),adverse reactions(ARs),changes in vital signs,and laboratory tests.RESULTS:JXP surpassed the placebo in reducing DRSP scores(psychological/somatic dysfunction)in the luteal phase over 3 menstrual cycles of treatment(PFAS=0.002,PPPS=0.001).Additionally,there were no significant differences in the incidence of AEs,severe AEs,withdrawal due to AEs and ARs between the two groups(all P>0.05),and no clinically significant adverse medical events related to the test drug observed.CONCLUSIONS:JXP was superior to the placebo in relieving the symptoms associated with PMS,which signified that JXP may be effective,safe,and welltolerated as an alternative therapy.
文摘Objective： To investigate the effects of 50% ethyl alcohol （EtOH） extracts from Danzhi Xiaoyao Pill （丹栀逍遥丸, DXP） on the proliferation of MCF-7 human breast cancer cells and potential mechanisms. Methods： ATP-Lite assay was performed to test the proliferation of the MCF-7 breast cancer cell line; and antioxidant activity was measured by the oxygen radical absorbance capacity （ORAC）. The effects of DXP on nitric oxide （NO） production were tested by lipopolysaccharide （LPS）- stimulated RAW 264.7 murine macrophages using the Griess reaction. Results： The 50% EtOH DXP extracts displayed a cytotoxic response on MCF-7 cells at 0.10, 0.25 and 0.50 mg/mL dosedependently with the proliferation inhibited by more than 85%. The ORAC value of the DXP was 820μ moL Trolox equivalent/g, about 40% of the vitamin C value. DXP extracts had significant inhibitory effect on NO production at the concentration from 0.0625 mg/mL to 0.5 mg/mL （P〈0.05, P〈0.01）. Conclusion： The extracts of DXP could significantly inhibit the proliferation of MCF-7 cells, with the effect possibly related to its antioxidant activity and the inhibition of NO production.
文摘目的探讨逍遥丸改善产前应激子代大鼠焦虑样行为的作用机制。方法采用随机数字表法选择28只SD子代大鼠,根据是否应激、灌胃不同药物,将大鼠分为对照组、产前应激组、产前应激+逍遥丸组和产前应激+氯化钠组,每组7只。然后通过旷场实验和高架十字实验观察各组子代大鼠的焦虑样行为,随后提取海马组织,分别采用荧光定量聚合酶链反应(PCR)和Western blot法观察谷氨酸受体A1亚基(GluA1)信使RNA(mRNA)和蛋白表达的变化。结果在旷场实验中,与对照组相比,产前应激组潜伏期明显延长,中央区时间百分比和运动距离明显减少(P<0.01);与产前应激+氯化钠组相比,产前应激+逍遥丸组子代大鼠潜伏期明显缩短,中央区时间百分比和运动距离明显增加[(55±14)s比(108±10)s、(14.8±2.4)%比(6.6±1.8)%、(2673±296)cm比(1747±353)cm](P<0.01);但各组子代大鼠运动速度比较差异无统计学意义(P>0.05)。在高架十字迷宫实验中,与对照组相比,产前应激组子代大鼠开放臂运动距离和开放臂时间百分比明显减少(P<0.01);与产前应激+氯化钠组相比,产前应激+逍遥丸组开放臂运动距离和开放臂时间百分比明显增加[(157±20)cm比(21±3)cm、(0.74±0.14)%比(0.50±0.15)%](P<0.01);但各组子代大鼠运动速度比较差异无统计学意义(P>0.05)。荧光定量PCR和Western blot法结果显示,与对照组相比,产前应激组子代大鼠海马中GluA1 mRNA和蛋白表达显著降低(P<0.01);与产前应激+氯化钠组相比,产前应激+逍遥丸组子代大鼠海马中GluA1 mRNA和蛋白表达显著升高(0.950±0.199 vs 0.491±0.181,0.663±0.047 vs 0.464±0.074)(P<0.01)。结论逍遥丸可明显改善产前应激子代大鼠焦虑样行为,其作用机制可能与提高海马区GluA1的表达有关。
