Effects of Inoculation with Phosphate Solubilizing Bacteria on the Physiology,Biochemistry,and Expression of Genes Related to the Protective Enzyme System of Fritillaria taipaiensis P.Y.Li

Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.

Study on regulating mechanisms of oxocrebanine obtained from Stephania hainanensis H.S.Lo et Y.Tsoong on microtubule sites and tubulin in human breast cancer MCF-7 cells

Objective:To determine the destructive ability of oxocrebanine,an anti-breast cancer active compound obtained from Stephania hainanensis H.S.Lo et Y.Tsoong,on microtubule network,and investigate the effect of oxocrebanine on microtubule network homeostasis at both molecular and cellular levels.Methods:the EBI site competition method and molecular docking method were used to determine the occupation of the microtubule site of oxocrebanine.Western Blot was used to detect the effect of oxocrebanine on microtubule-associated proteins including STAT3,PAK1,CAMK4,and PKA.Results:The results of EBI site competition assay showed that the binding of EBI toβ-Tubulin covalent fusions produced adducts that appeared in regions of lower molecular weight thanβ-tubulin(ctrl 2).Molecular docking results showed that oxocrebanine could occupy the colchicine site of microtubule proteins.As revealed by Western Blot,the expression of STAT3 protein was decreased after MCF-7 cells have been treated with low,medium,and high concentration of oxocrebanine or the positive drug taxol for 48 h(P<0.01).The expression levels of PAK1 and Camk4 proteins aslo showed significant reductions(P<0.05,or P<0.01).Oxocrebanine also decreased the PKA protein in MCF-7 cells compared to the control group(P<0.01).Conclusions:Oxocrebanine,a ligand that binds at the colchicine site of tubulin,perturbs tubulin polymerization and causes mitosis in MCF-7 cells,thus leading to MCF-7 cell death.Oxocrebanine may promote microtubule dynamics through stathmin by inhibiting the expression levels of STAT3,PAK1,Camk4,and PKA proteins in MCF-7 cells.Oxocrebanine interfers with spindle formation,and ultimately causes mitotic catastrophe in MCF-7 cells.

用Y.pestis接种某些植物的研究

本研究采用鼠疫弱毒株061号对大豆、小麦、兴安胡枝子、紫苜蓿、青蒿等进行接种,在不同时期从大豆组织中分离到目的菌,并发现其菌落形态由R型向S型改变,其营养需求也发生了明显的变化。

Y.Meyer小波的一般形式(英文)

首先我们证明了 ,如果尺度函数有紧支集 ,来自多尺度分析的小波函数的支集形式 .然后我们证明了 Y.Meyer小波的尺度函数的一般形式 .最后我们给出了它的另外两种形式和对应的 Y.Meyer小波 .

关于W.Y.Vélez猜想Ⅱ

设 k为域 ,本文继续讨论了文 [1 ]中提出的 W.Y.Vélez问题 ,在基域 k中不含有 m次本原单位根时 ,给出了该问题成立的一个条件 ,推广了文 [2

“X_1是Y...X_2VP”结构中人称代词的间接回指分析

基于Jackendoff的平行构架,探讨"X_1是Y...X_2VP"假设复句中的人称代词间接回指现象。"X_1是Y...X_2VP"结构所表述的概念结构分为描述层和指称层,人称代词在两个层面的回指也有不同表现,与其句法结构之间存在不匹配现象。文章把认知系统中各结构成分之间的这种非严格对应关系视为句法结构和语义之间的弱接口所致。

Y.M.C.A.与Y.W.C.A.

Y.M.C.A.(Young Men’s Christian Association)意思是“基督教青年会”。该组织是在乔治·威廉斯爵士(Sir George Williams)倡导下,于1844年在英国伦敦建立的以通过团体活动和公民训练发扬基督徒高尚品德为宗旨的跨教派的非政治性组织。Williams 是伦敦一家布店店员,后来成为店主。起初该组织是一个俱乐部,拥有12名青年成员,他们提出的口号是:“提高布店和其他行业青年的灵性修养”。这个俱乐部迅速推广到英国各地。1850年传到澳大利亚,1851年传到北美洲,其后在北美洲各地得到空前发展。

基于电信级以太网OAM的Y.1731应用

Y.1731是一个建立服务以太网OAM功能的标准,用来提供电信级以太网的运营、维护和管理能力。本文重点讲述了Y.1731协议在以太网链路层中的端到端链路的故障确认与定位。在这项技术中创新地在Linktrace功能中采用了反向搜索方法对LTR信息节点进行排序、删除,提高了输出信息的准确性,同时也提高了故障检测与故障定位的效率。

Y.Meyer型小波的构造

Y.Meyer小波不仅具有快速衰减和无穷可微的性质,而且在频域具有紧支性。利用多分辨分析理论及AWM方法,给出了Y.Meyer型小波一般形式构造的具体方法及过程,使构造方法规范化,并保持了它在频域的紧支性及自身的快速衰减和无穷可微的性质。

NTP协议在Y.1731中的应用研究

随着信息技术和网络技术的发展,人们对时间同步的精度要求越来越高。网络时间协议(NTP,Network Time Protocol)是一种基于UDP的时间同步协议。介绍了NTP协议的基本原理,Y.1731单端DM原理。通过NTP协议在Y.1731报文中的应用研究和实践,使传统的网络时间同步精度能达到毫秒级别。实验数据表明,文中所设计的网络时钟同步系统比传统的时钟同步具有更高的时钟同步精度。

M.Y.Lab木艺实验室:给马云上过课的木艺坊

36位CEO齐聚杭州M.Y.Lab木艺实验室做木工由阿里巴巴集团董事局主席马云牵头成立的湖畔大学2015年于3月25日开学,首期36位来自不同领域的企业家全部齐聚杭州。这36位企业家来自不同发展阶段的企业,既有明星企业家,也有新锐创业家,包括俏江南总裁汪小菲、百合网联合创始人慕岩、汽车之家CEO秦致、美柚CEO陈方毅等。开学第一天36人先上木工课,每6人一个小组制作一把木琴。

影片《C.R.A.Z.Y.》中的信仰探寻

加拿大电影《C.R.A.Z.Y.》表现了亲情、伦理、信仰、自我认同等丰富的主题,其中对基督教信仰的探寻是很重要的一条线索。影片对主人公Zach心理经历的追述,正暗合了基督教中的具有"相信—怀疑—坚定信仰"结构的信仰观。

改良联苯胺黄P.Y.12在水性柔版印刷油墨中的应用

以联苯胺黄P.Y.12改良为出发点,通过改变偶合方式、添加表面活性剂以及粒径处理等3种典型的处理方式对联苯胺黄P.Y.12进行处理,制备出一系列不同的联苯胺黄P.Y.12的改性产品剂型.对处理后的联苯胺黄P.Y.12进行应用性能表征,从中选择最优条件,制备出可以应用于水性印刷体系的联苯胺黄P.Y.12产品.

挖掘都市之中的隐匿技艺 M.Y.Lab上海店空间改造

M.Y.Lab木艺实验室上海店位于上海市长宁区,原东风沙发厂厂房一层,单层面积300 m^2,边上有新增的150 m^2单层附房,附房和界墙之间形成很小的三角形场地。业主希望能把原有的单层厂房隔成两层,作为木作培训的体验性商业空间使用。经过对现场的勘测,设计师决定在这个5 m高的厂房空间里植入一个＂考古挖掘现场＂。将主要的木艺操作空间做下沉处理,隐藏在入口的墙内,并用纯净的水磨石矮墙围合,创造出隐蔽的神秘空间。＂

Analysis on Current Situations of Endangered P.intermedius S.Y.Chen

We carried out a general survey of current situations of P.intermedius S.Y.Chen.Results show that P.intermedius S.Y.Chen is distributed in Dongkeng Township,Jingnan Township and Yanxi Township.It covers an area of 262.51 hm2.The distribution areas are narrow and limited.What's worse,it suffers from serious destruction and has been withered in large areas.Besides,according to current introduction test,it is difficult to survive in other places.In accordance with Exploring the Criteria for Endangered Categories of Bamboo Species from China put forward by Ma Naixun et al from the Research Institute of Subtropical Forestry of Chinese Academy of Forestry(RISFCAF),we propose incorporating P.intermedius S.Y.Chen into vulnerable species for protection.

Nutrition Bowl"Double Covering"Cutting Propagation Technique for Llex cornuta var.fortunei S.Y.Hu

Llex cornuta var.fortunei S.Y.Hu is a good ornamental tree species for viewing fruits and leaves,and has a wide range of garden uses.Nutrition bowl cutting seedlings can improve the survival rate of L.cornut a var.fortunei S.Y.Hu transplanting and greening,and has the advantages of no time limit for planting and saving management costs.Based on this,this paper introduced the container seedling double-covering cutting technology for L.cornut a var.fortunei,to provide a reference for the propagation of L.cornut a var.fortunei.

浅析中学动漫的E.A.S.Y.教学模式

动漫在整个社会生活及文化产业中的地位不断提升,中学的动漫教育目前还缺乏针对性的教育。为对传统的动漫教学模式进行创新,探索动漫教学的E.A.S.Y.教学模式,来加快动漫课程的实践化、科学化、全面化。

挖掘都市之中的隐匿技艺——M.Y.Lab上海店空间改造

1设计的开始M.Y.Lab木艺实验室上海店位于上海市长宁区原东风沙发厂厂房一层,单层面积300m^2,边上有新增的150m2单层附房,附房和界墙之间形成很小的三角形场地。业主希望能把原有300m^2的单层厂房分隔成两层,作为木作培训的体验性商业空间使用。2初见场地的印象初到场地,印象最深的不是空间本身,而是破败厂房建筑旁仅隔一道围墙的地铁高架线路。

中国汽车设计C.Y.E

C级车的困惑 汽车设计不同于艺术是因为艺术可以以完全主观的形式存在可以标新立异。和工业设计相同汽车设计都是要讲求市场效益的。所以笔者个人认为汽车造型更多要寻求一种为相当数量的人所能接受的美。车型的产量越大其设计就要迎合更多的人的胃口,这也就是为什么年产百万辆级的大众高尔夫的设计相对于法拉利的设计显得平淡。法拉利有着非常目标化的市场群体,拥有财富,讲求个性。