Two control diets based on the commercial formula were designed to contain high(27%,D1)and low(22%,D2)levels of fish meal,respectively.Into D2,500,1000 and 1500 mg kg 1 of yeast extract were added,respectively,yieldin...Two control diets based on the commercial formula were designed to contain high(27%,D1)and low(22%,D2)levels of fish meal,respectively.Into D2,500,1000 and 1500 mg kg 1 of yeast extract were added,respectively,yielding three experimental diets(YE1 through YE3).Shrimp(initial body weight 0.30 g±0.02 g)were fed with the experimental diets,five tanks each diet and 30 shrimp individuals each tank,for 8 weeks,and then challenged with Vibrio parahaemolyticus.The results showed that the specific growth rate(SGR)of shrimp in D2 was significantly lower than that of shrimp in D1(P<0.05).The SGR of shrimp in YE3 was similar to that of shrimp in D1.The feed intake of shrimp was similar between D1 and D2.The feed conversion ratio and protein efficiency ratio of shrimp were similar among all diets(P>0.05).YE significantly improved the activity of glutathione S-transferase.The concentration of glutathione(GSH)and the total serum anti-oxidative capacity(T-AOC)of D1 were significantly higher than those of shrimp feeding other diets(P<0.05).The content of serum malondialdehyde of shrimp feeding YE2 and YE3 was signifi-cantly lower than that of shrimp feeding D2(P<0.05).The thickness of intestine muscular layer of shrimp feeding YE1 and YE2 was similar to that of shrimp feeding D1.The shrimp feeding YE1 showed the highest villus height of intestine among all groups.The cumulative mortality after challenging was similar among all groups(70.00%86.67%)(P>0.05).In conclusion,1000 1500 mg kg 1 of YE was suggested to be supplemented into the practical diets to improve the growth,anti-oxidative capacity and intestinal mor-phology of shrimp L.vannamei.展开更多
[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biolog...[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biology,and discussed the application of the Growgx yeast fermentation product extract for skin aging.[Methods]A photo-aging model of HFF-1 cells was induced by UVA radiation meter,and it was given different concentrations of Growgx for intervention.The proliferation activity of the HFF-1 cells was detected with MTT method,and their migration and invasion ability was measured by scratch test.The SOD,HYP and MDA levels were detected with corresponding kits.After oxygen infusion apparatus assisted facial skin administration,the repair of the skin was observed through the VISIA skin tester.[Results]The survival rate of HFF-1 cells was reduced significantly by UVA with an irradiation dose of 10.8 J/cm2(P<0.05),while Growgx significantly increased their survival rate(P<0.001)and sped up the repair of damaged cells.Growgx promoted the production of SOD(P<0.05),reduced the level of MDA(P<0.05),and increased the level of HYP(P<0.05).Growgx effectively inhibited UVA-induced photo-aging of HFF-1 cells.The mechanism may be related to accelerating cell damage repair,up-regulating SOD and HYP expression levels,and reducing MDA expression level.The clinical observation showed that Growgx effectively improved skin spots and pores,making the skin smoother and more delicate.[Conclusions]Growgx can effectively fight against photo-aging caused by ultraviolet rays,and can significantly improve skin wrinkles.展开更多
β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as...β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as (in)solubility, viscosity, and gelation, β-glucans are increasingly being used in the food, pharmaceutical, and cosmetic industries. The purpose of this review is to provide an overview of the different types of β-glucans, their sources, especially Saccharomyces cerevisiae yeasts, and the methods of extraction, isolation, and purification of β-glucans, with the aim of optimizing these methods for the efficient production process. Moreover, the physico-chemical properties, modifications, current applications and future prospects of the use of β-glucans in food, medicines, cosmetics and other potential value-added products are summarized. The data presented indicate that β-glucans will play an increasingly important role in the sector of special-purpose food products as well as in other current and future areas.展开更多
基金financially supported by grants from the Marine Economic Innovation and Development Regional Model City Project (2016) of Qingdao,Chinathe Key R&D Program of Shandong Province, China (Nos. 2017CXGC0105, 2016CYJS04A01)
文摘Two control diets based on the commercial formula were designed to contain high(27%,D1)and low(22%,D2)levels of fish meal,respectively.Into D2,500,1000 and 1500 mg kg 1 of yeast extract were added,respectively,yielding three experimental diets(YE1 through YE3).Shrimp(initial body weight 0.30 g±0.02 g)were fed with the experimental diets,five tanks each diet and 30 shrimp individuals each tank,for 8 weeks,and then challenged with Vibrio parahaemolyticus.The results showed that the specific growth rate(SGR)of shrimp in D2 was significantly lower than that of shrimp in D1(P<0.05).The SGR of shrimp in YE3 was similar to that of shrimp in D1.The feed intake of shrimp was similar between D1 and D2.The feed conversion ratio and protein efficiency ratio of shrimp were similar among all diets(P>0.05).YE significantly improved the activity of glutathione S-transferase.The concentration of glutathione(GSH)and the total serum anti-oxidative capacity(T-AOC)of D1 were significantly higher than those of shrimp feeding other diets(P<0.05).The content of serum malondialdehyde of shrimp feeding YE2 and YE3 was signifi-cantly lower than that of shrimp feeding D2(P<0.05).The thickness of intestine muscular layer of shrimp feeding YE1 and YE2 was similar to that of shrimp feeding D1.The shrimp feeding YE1 showed the highest villus height of intestine among all groups.The cumulative mortality after challenging was similar among all groups(70.00%86.67%)(P>0.05).In conclusion,1000 1500 mg kg 1 of YE was suggested to be supplemented into the practical diets to improve the growth,anti-oxidative capacity and intestinal mor-phology of shrimp L.vannamei.
基金Supported by General Program of Guangxi Natural Science Foundation(2018GXNSFAA138098).
文摘[Objectives]To explore the effect of the extract of Growgx yeast fermentation product on ultraviolet A(UVA)-induced photo-aging of human skin fibroblasts(HFF-1)and its specific mechanisms from the level of cell biology,and discussed the application of the Growgx yeast fermentation product extract for skin aging.[Methods]A photo-aging model of HFF-1 cells was induced by UVA radiation meter,and it was given different concentrations of Growgx for intervention.The proliferation activity of the HFF-1 cells was detected with MTT method,and their migration and invasion ability was measured by scratch test.The SOD,HYP and MDA levels were detected with corresponding kits.After oxygen infusion apparatus assisted facial skin administration,the repair of the skin was observed through the VISIA skin tester.[Results]The survival rate of HFF-1 cells was reduced significantly by UVA with an irradiation dose of 10.8 J/cm2(P<0.05),while Growgx significantly increased their survival rate(P<0.001)and sped up the repair of damaged cells.Growgx promoted the production of SOD(P<0.05),reduced the level of MDA(P<0.05),and increased the level of HYP(P<0.05).Growgx effectively inhibited UVA-induced photo-aging of HFF-1 cells.The mechanism may be related to accelerating cell damage repair,up-regulating SOD and HYP expression levels,and reducing MDA expression level.The clinical observation showed that Growgx effectively improved skin spots and pores,making the skin smoother and more delicate.[Conclusions]Growgx can effectively fight against photo-aging caused by ultraviolet rays,and can significantly improve skin wrinkles.
文摘β-glucans are bioactive compounds with a wide range of biological properties, including anticancer, anti-inflammatory, antioxidant, and immune-modulating properties. Due to their specific physical properties, such as (in)solubility, viscosity, and gelation, β-glucans are increasingly being used in the food, pharmaceutical, and cosmetic industries. The purpose of this review is to provide an overview of the different types of β-glucans, their sources, especially Saccharomyces cerevisiae yeasts, and the methods of extraction, isolation, and purification of β-glucans, with the aim of optimizing these methods for the efficient production process. Moreover, the physico-chemical properties, modifications, current applications and future prospects of the use of β-glucans in food, medicines, cosmetics and other potential value-added products are summarized. The data presented indicate that β-glucans will play an increasingly important role in the sector of special-purpose food products as well as in other current and future areas.
