Hansenula anomala were general pale yellow oval-shaped bacteria in aerobic biochemical wastewater process,but transformed into pale yellow round-shape under hypoxia condition.A better precipitation of lysine wastewate...Hansenula anomala were general pale yellow oval-shaped bacteria in aerobic biochemical wastewater process,but transformed into pale yellow round-shape under hypoxia condition.A better precipitation of lysine wastewater could be obtained by using Hansenula anomala in hypoxia biochemical treatment.Therefore,the lysine wastewater could be used to produce yeast protein feed at 23-33 ℃ water temperature and 2.8-4.4 p H by Hansenula anomala in hypoxia condition.The results indicated that removal rate of chemical oxygen demand（COD） was 65.8%,and that of suspended substance（SS） was 95.6%.Then the deposited yeast protein was dried after secondary sedimentation and steam sandwich concentrated method.Results showed that 3.9 t yeast protein feed could be obtained every day,and its price was 3 000 Yuan per ton,so that the annual profit of company would be 9.6 million Yuan after deducting the operating cost of wastewater treatment.展开更多
啤酒酵母泥以异丙醇破壁处理,以磷酸钾缓冲液提取超氧化物歧化酶(SOD),通过单因素和正交实验优化了提取条件。结果表明,提取SOD的最佳条件是:以100%浓度异丙醇破壁处理150 min,以pH 5.0磷酸钾缓冲液为提取剂,液料比36 m L/g。在此条件下...啤酒酵母泥以异丙醇破壁处理,以磷酸钾缓冲液提取超氧化物歧化酶(SOD),通过单因素和正交实验优化了提取条件。结果表明,提取SOD的最佳条件是:以100%浓度异丙醇破壁处理150 min,以pH 5.0磷酸钾缓冲液为提取剂,液料比36 m L/g。在此条件下,SOD的得率为1 257.2 U/g。纯化后的SOD酶液主要含有分子量大小为25.46 k D和49.53 k D两种蛋白,其中分子量25.46 KD的蛋白与NCBI蛋白质数据库中baker’s yeast中SOD的分子量相符合,该酶理论等电点为8.49,与中文文献中报道的SOD均不同,是一种新型SOD。展开更多
文摘Hansenula anomala were general pale yellow oval-shaped bacteria in aerobic biochemical wastewater process,but transformed into pale yellow round-shape under hypoxia condition.A better precipitation of lysine wastewater could be obtained by using Hansenula anomala in hypoxia biochemical treatment.Therefore,the lysine wastewater could be used to produce yeast protein feed at 23-33 ℃ water temperature and 2.8-4.4 p H by Hansenula anomala in hypoxia condition.The results indicated that removal rate of chemical oxygen demand（COD） was 65.8%,and that of suspended substance（SS） was 95.6%.Then the deposited yeast protein was dried after secondary sedimentation and steam sandwich concentrated method.Results showed that 3.9 t yeast protein feed could be obtained every day,and its price was 3 000 Yuan per ton,so that the annual profit of company would be 9.6 million Yuan after deducting the operating cost of wastewater treatment.
文摘啤酒酵母泥以异丙醇破壁处理,以磷酸钾缓冲液提取超氧化物歧化酶(SOD),通过单因素和正交实验优化了提取条件。结果表明,提取SOD的最佳条件是:以100%浓度异丙醇破壁处理150 min,以pH 5.0磷酸钾缓冲液为提取剂,液料比36 m L/g。在此条件下,SOD的得率为1 257.2 U/g。纯化后的SOD酶液主要含有分子量大小为25.46 k D和49.53 k D两种蛋白,其中分子量25.46 KD的蛋白与NCBI蛋白质数据库中baker’s yeast中SOD的分子量相符合,该酶理论等电点为8.49,与中文文献中报道的SOD均不同,是一种新型SOD。
