Characterization and antioxidant activities of marine pepsin soluble collagen from the skin of yellow goosefish Lophius litulon

Characteristics and antioxidant activities of pepsin-soluble collagen(PSC) from yellow goosefish( Lophius litulon) skins were investigated. PSC was characterized as a type I collagen, and its imino acid content was 193 residues/1 000 residues. PSC's denaturation temperature was ~17.56°C and Fourier transform infrared spectra confirmed the presence of triple helices. Solubility analysis showed good solubility at acidic pH(1–6) or low Na Cl concentrations(≤2%). PSC showed scavenging activity against hydroxyl radicals and superoxide anions in a concentration-dependent manner. Furthermore, PSC could protect D-galactose-induced skin aging by significantly controlling malondialdehyde formation and improving the activity of superoxide dismutase, glutathione peroxidase, catalase, glutathione, and hydroxyproline. PSC may be a promising antioxidant in appropriate applications.

黄鮟鱇繁殖生物学研究

以2008年9月至2009年7月在黄海南部捕获的644尾黄鮟鱇(Lophius litulon)为对象,对该种鱼的繁殖生物学进行了研究。结果表明,所采样本性比接近于1︰1(2=2.61,P>0.05);但全长在251～400mm范围时,雄性个体数量远超过雌性(P<0.01);全长在450～550mm范围时,雌性个体数量多于雄性(P<0.01);全长大于550mm范围时,个体均为雌性;冬季,雄性个体数量占绝对优势(P<0.01)。利用逻辑斯蒂拟合方程估算的黄鮟鱇50%个体初次性成熟全长L50,雄性为332mm,雌性为483mm。通过对性腺指数的月变化和不同卵巢发育阶段雌性个体所占比例的分析,初步推测黄鮟鱇产卵时期为2-6月份,繁殖盛期为3-4月份;其绝对繁殖力为(614820±253800)粒,相对繁殖力为(244±43)粒/g。黄鮟鱇不同性腺发育阶段的肝脏指数存在显著变化,雌性,F=9.97、P<0.01;雄性,F=4.48、P<0.01,肝脏在该鱼种卵巢发育过程中可能起到了能量供给作用。鮟鱇类资源对捕捞的承受压力极其脆弱,50%个体初次性成熟全长可作为其资源管理的基础生物学指标。本研究旨在为黄鮟鱇的种群数量变动研究和渔业管理提供基础资料。