Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically st...Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically stable yellow-green leaf mutant ygl16 was identified from mutated“Xinong 1B”.Compared with the wild type,the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly.The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts,and the grana and matrix lamellae were blurred and loose in varied degrees,and the chloroplast structure was disordered,while the osmiophilic corpuscles increased.The results of the genetic analysis and mapping showed that the phenotype of ygl16 was controlled by a pair of recessive nuclear gene.The gene located in the 56Kb interval between RM25654 and R3 on the long arm of chromosome 10.The sequencing results showed that the 121st base of the first intron of the candidate gene OsPORB/FGL changed from A to T in the interval.qRT-PCR results showed that the expression of chlorophyll synthase-related genes in the mutant decreased.展开更多
基金supported by grants from the Project of Creating High Quality,Disease Resistance and High Combining Ability CMS Lines(Grant No.cstc2018jscx-msybX0250)Chongqing Technology Innovation and Application Demonstration Project and the Project of High Photosynthetic Efficiency Rice Breeding Technology System(Grant No.2017YFD0100201)the National Key Research and Development Program“Seven Crops Breeding”.
文摘Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis,photomorphogenesis,hormone physiology and disease resistance.In this study,the genetically stable yellow-green leaf mutant ygl16 was identified from mutated“Xinong 1B”.Compared with the wild type,the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly.The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts,and the grana and matrix lamellae were blurred and loose in varied degrees,and the chloroplast structure was disordered,while the osmiophilic corpuscles increased.The results of the genetic analysis and mapping showed that the phenotype of ygl16 was controlled by a pair of recessive nuclear gene.The gene located in the 56Kb interval between RM25654 and R3 on the long arm of chromosome 10.The sequencing results showed that the 121st base of the first intron of the candidate gene OsPORB/FGL changed from A to T in the interval.qRT-PCR results showed that the expression of chlorophyll synthase-related genes in the mutant decreased.
