YN87448 virus strain was isolated from a fevered female patient (52 years old ) in Yunnan Province in 1986, and was identified as a member of Alphavirus using serological method. One primer was designed from common ha...YN87448 virus strain was isolated from a fevered female patient (52 years old ) in Yunnan Province in 1986, and was identified as a member of Alphavirus using serological method. One primer was designed from common hairpin conserved region of Alphavirus. Two fragments were amplified by single primer differential RT PCR, and they were cloned into pGEM T vector. Sequences were determined, and then analyzed by Software and GenBank. Results showed that the 1118pb long fragment was highly homologous to the sequence of Sindbis like virus S.A.A.R86. The homogeneity of the 1118bp fragment between YN87448 virus strain and S.A.A.R86 virus strain was 98%. Single primer differential RT PCR is a useful method with simple, economic, and practical value for Alphavirus identification.展开更多
目的实践观察对比环境温度对Welch Al yn耳温仪侧耳温的影响。方法随机抽取2014年1~2月急诊内科、急诊儿科就诊的部分配合测量患者,评估入院是否受环境温度影响,等待1~5min与20min同侧耳温对比,并随机进行左右耳温的对比分析观察。结果...目的实践观察对比环境温度对Welch Al yn耳温仪侧耳温的影响。方法随机抽取2014年1~2月急诊内科、急诊儿科就诊的部分配合测量患者,评估入院是否受环境温度影响,等待1~5min与20min同侧耳温对比,并随机进行左右耳温的对比分析观察。结果患者不同入院方式等待1~5min侧耳温与等待20min侧耳温两组之间温度明显差异有统计学意(<0.001),患者左右耳温度差异无统计学意义(>0.05)。结论德国Welch Al yn公司生产的红外线鼓膜耳温仪测量耳温,在急诊预检,要充分评估有无受环境温度的影响,过冷或过热的环境及捂耳和耳受压情况下,须等待20min测量耳温能较好地反应患者体核温度。耳温仪操作简单、方便、快捷、安全、准确,值得临床护理推广使用。展开更多
为更好地了解勤奋金属球菌(Metallosphaera sedula)作用下沉淀的累积进程及沉淀的特性,将一株在我国首次分离鉴定的勤奋金属球菌(YN23)接种在以Fe^(2+)为能源的培养基中,于该菌最佳生长条件(pH1．5,53℃,0．2g·l_(-1)酵母提取物,30...为更好地了解勤奋金属球菌(Metallosphaera sedula)作用下沉淀的累积进程及沉淀的特性,将一株在我国首次分离鉴定的勤奋金属球菌(YN23)接种在以Fe^(2+)为能源的培养基中,于该菌最佳生长条件(pH1．5,53℃,0．2g·l_(-1)酵母提取物,30g·1_(-1)Fe_2SO_4·7H_2O and 170rpm)下培养。接种25h后,当Fe^(2+)氧化率达到90%时,开始出现沉淀,pH也达到1．92的最高值;到第95h,当沉淀累积到7．9g·1^(-1),时,沉淀反应停止,此时pH达到1．32的最低点。菌群密度随着Fe^(2+)的氧化,前期快速增长;当沉淀出现以后,随着沉淀的累积,逐渐降低。X衍射图谱、红外吸收光谱、能谱和扫描电镜数据揭示,YN23菌株合成的沉淀是黄钾铁矶和黄铵铁矾的混合物,形态特征更接近于后者。展开更多
文摘YN87448 virus strain was isolated from a fevered female patient (52 years old ) in Yunnan Province in 1986, and was identified as a member of Alphavirus using serological method. One primer was designed from common hairpin conserved region of Alphavirus. Two fragments were amplified by single primer differential RT PCR, and they were cloned into pGEM T vector. Sequences were determined, and then analyzed by Software and GenBank. Results showed that the 1118pb long fragment was highly homologous to the sequence of Sindbis like virus S.A.A.R86. The homogeneity of the 1118bp fragment between YN87448 virus strain and S.A.A.R86 virus strain was 98%. Single primer differential RT PCR is a useful method with simple, economic, and practical value for Alphavirus identification.
文摘目的实践观察对比环境温度对Welch Al yn耳温仪侧耳温的影响。方法随机抽取2014年1~2月急诊内科、急诊儿科就诊的部分配合测量患者,评估入院是否受环境温度影响,等待1~5min与20min同侧耳温对比,并随机进行左右耳温的对比分析观察。结果患者不同入院方式等待1~5min侧耳温与等待20min侧耳温两组之间温度明显差异有统计学意(<0.001),患者左右耳温度差异无统计学意义(>0.05)。结论德国Welch Al yn公司生产的红外线鼓膜耳温仪测量耳温,在急诊预检,要充分评估有无受环境温度的影响,过冷或过热的环境及捂耳和耳受压情况下,须等待20min测量耳温能较好地反应患者体核温度。耳温仪操作简单、方便、快捷、安全、准确,值得临床护理推广使用。
基金Foundation item:Chinese Science Foundation for Distinguished Group(50621063)National Basic Research Program(973 Program)of P.R.China(2004CB619201)
文摘为更好地了解勤奋金属球菌(Metallosphaera sedula)作用下沉淀的累积进程及沉淀的特性,将一株在我国首次分离鉴定的勤奋金属球菌(YN23)接种在以Fe^(2+)为能源的培养基中,于该菌最佳生长条件(pH1．5,53℃,0．2g·l_(-1)酵母提取物,30g·1_(-1)Fe_2SO_4·7H_2O and 170rpm)下培养。接种25h后,当Fe^(2+)氧化率达到90%时,开始出现沉淀,pH也达到1．92的最高值;到第95h,当沉淀累积到7．9g·1^(-1),时,沉淀反应停止,此时pH达到1．32的最低点。菌群密度随着Fe^(2+)的氧化,前期快速增长;当沉淀出现以后,随着沉淀的累积,逐渐降低。X衍射图谱、红外吸收光谱、能谱和扫描电镜数据揭示,YN23菌株合成的沉淀是黄钾铁矶和黄铵铁矾的混合物,形态特征更接近于后者。
