学校大量服务器使用Cent OS做操作系统,但大多数服务器都无法连接Internet,无法联机更新操作系统补丁。即使允许这些服务器连接Internet更新操作系统,也会带来大量重复的网络流量下载问题,因此在学校内部署一台Cent OS YUM源服务器是合...学校大量服务器使用Cent OS做操作系统,但大多数服务器都无法连接Internet,无法联机更新操作系统补丁。即使允许这些服务器连接Internet更新操作系统,也会带来大量重复的网络流量下载问题,因此在学校内部署一台Cent OS YUM源服务器是合适的,既可以为Cent OS服务器提供本地YUM源,又可以减少重复网络流量下载问题。本文分析比较了搭建Cent OS YUM源服务器的方法,并实际搭建了一台Cent OS YUM源服务器。文中的方法实用准确,为今后Cent OS YUM源服务器的搭建提供了重要的参考意义。展开更多
OBJECTIVE: To investigate the effect of Young Yum pill (YYP) on inflammatory mediators in cultured RAW 264.7 cells and elucidate the nuclear factor- kappa B (NF-κB)-related mechanism behind the action. METHODS: YYP w...OBJECTIVE: To investigate the effect of Young Yum pill (YYP) on inflammatory mediators in cultured RAW 264.7 cells and elucidate the nuclear factor- kappa B (NF-κB)-related mechanism behind the action. METHODS: YYP was extracted with 95% ethanol Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages were used to evaluate the effect of YYP on inflammatory mediators. Production of nitric oxide (NO) and prostaglandin E2 (PGE2) were measured by Griess test and enzyme-linked immunosorbent assay, respectively. The levels of genes and proteins involved in the generation of inflammatory mediators were examined using real-time polymerase chain reaction and Western blotting, respectively. RESULTS: YYP dose-dependently suppressed LPS-induced production of NO, PGE2 and tumor necrosis factor-α(TNF-α), and elevation of mRNA and protein levels of inducible NO synthase and cyclooxygenase- 2 in RAW 264.7 macrophages. These observations were associated with decreased NF-κB p65 phosphorylation and nuclear localization, enhanced Akt (protein kinase B) phosphorylation, as well as reduced inhibitor of κB (IκB)α degradation and IκB kinase α/β phosphorylation. CONCLUSION: The present study demonstrated an inhibitory effect of YYP on the NF-κB-regulated inflammatory mediators NO, PGE2 and TNF-α in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for the use of YYP in treating inflammatory disorders.展开更多
文摘学校大量服务器使用Cent OS做操作系统,但大多数服务器都无法连接Internet,无法联机更新操作系统补丁。即使允许这些服务器连接Internet更新操作系统,也会带来大量重复的网络流量下载问题,因此在学校内部署一台Cent OS YUM源服务器是合适的,既可以为Cent OS服务器提供本地YUM源,又可以减少重复网络流量下载问题。本文分析比较了搭建Cent OS YUM源服务器的方法,并实际搭建了一台Cent OS YUM源服务器。文中的方法实用准确,为今后Cent OS YUM源服务器的搭建提供了重要的参考意义。
基金Supported by Wai Yuen Tong Medicine Company Limited,Innovation and Technology Fund(UIT/315)General Research Fund(GRF:12125116)of Hong KongFRG1/16-17/048 and FRG2/16-17/033 from the Hong Kong Baptist University
文摘OBJECTIVE: To investigate the effect of Young Yum pill (YYP) on inflammatory mediators in cultured RAW 264.7 cells and elucidate the nuclear factor- kappa B (NF-κB)-related mechanism behind the action. METHODS: YYP was extracted with 95% ethanol Lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages were used to evaluate the effect of YYP on inflammatory mediators. Production of nitric oxide (NO) and prostaglandin E2 (PGE2) were measured by Griess test and enzyme-linked immunosorbent assay, respectively. The levels of genes and proteins involved in the generation of inflammatory mediators were examined using real-time polymerase chain reaction and Western blotting, respectively. RESULTS: YYP dose-dependently suppressed LPS-induced production of NO, PGE2 and tumor necrosis factor-α(TNF-α), and elevation of mRNA and protein levels of inducible NO synthase and cyclooxygenase- 2 in RAW 264.7 macrophages. These observations were associated with decreased NF-κB p65 phosphorylation and nuclear localization, enhanced Akt (protein kinase B) phosphorylation, as well as reduced inhibitor of κB (IκB)α degradation and IκB kinase α/β phosphorylation. CONCLUSION: The present study demonstrated an inhibitory effect of YYP on the NF-κB-regulated inflammatory mediators NO, PGE2 and TNF-α in LPS-stimulated RAW 264.7 macrophages, providing a pharmacological basis for the use of YYP in treating inflammatory disorders.
