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Bioactivities of Extracts from Toona sinensis against Sitophilus zeamais
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作者 陈琳 《Plant Diseases and Pests》 CAS 2010年第5期47-49,共3页
[ Objective ] The repellent activities and contact toxicity of extracts from Toona sinensis against Sitophilus zeamais were studied. [ Meth- od] 95% alcohol, petroleum ether and dichloromethane were selected to extrac... [ Objective ] The repellent activities and contact toxicity of extracts from Toona sinensis against Sitophilus zeamais were studied. [ Meth- od] 95% alcohol, petroleum ether and dichloromethane were selected to extract the active components from the leaves and barks of T. sinensis, respectively. And then the extracts were prepared at 0.6, 0.3, 0.15 g/ml to study the biological activity against S. zeamais in lab. [ Result J The petroleum ether extracts from the leaves of T. sinensis had significantly repellent effects on S. zeamais, but the extracts from T. sinensis with three solvents had no obvious contact toxicity against S. zeamais. [ Conclusion ] The petroleum ether extracts from S. zeamais had certain repellent activity. 展开更多
关键词 Toona sinensis(Roem) EXTRACT Sitophilus zeamais Repellent action Contact toxicity
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Optimum Insecticide Doses Determination of Seed Oil of Three Botanicals Jatropha Curcas L., Helianthus Annus L. and Cocos Nucifera L. against Maize Weevil Sitophilus Zeamais Mots.
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作者 Rachael Folake Ogunleye Mary Omofolarin Ogunkoya Francis Olawale Abulude 《Journal of Life Sciences》 2011年第4期298-301,共4页
The insecticidal effect of four dosage rates of three botanicals namely Jatropha curcas, Heliathus annus and Cocos nucifera was tested on the maize weevil Sitophilus zeamais Mots.. This is done for the purpose of find... The insecticidal effect of four dosage rates of three botanicals namely Jatropha curcas, Heliathus annus and Cocos nucifera was tested on the maize weevil Sitophilus zeamais Mots.. This is done for the purpose of finding a replacement for conventional insecticides which has been found to be harmful to man. The seed oil was applied topically at the rate of 0.1, 0.2, 0.3 and 0.4 mL per insect. There were a total of 20 insects per Petri-dish. There were four replicates per treatment. Insect mortality was recorded on 12 hourly basis for 48 hours. The results of insects treated with all dosage rates of C. nucifera showed a significantly higher mortality when compared with the control. In the case ofH. annus, insect mortality ranged from 40-100, 70-100, 60-100 and 80-100% and for J. curcas the result ranged from 0.0-100, 40-100, 80-100 and 80-100% for rates of 0.1, 0.2, 0.3 and 0.4 mL, respectively, from 12 hrs to 48 hrs post application. The control experiment remained at 0% level throughout the period of the experiments. 展开更多
关键词 Seed oil botanicals insecticides Sitophilus zeamais.
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Evaluation of the dust and methanol extracts of Garcinia kolae for the control of Callosobruchus maculatus (F.) and Sitophilus zeamais (Mots) 被引量:1
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作者 OGUNLEYE R.F. ADEFEMI S.O. 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2007年第12期912-916,共5页
Insecticidal effects of different doses of the dust and methanol extracts of Garcinia kolae on Callosobruchus maculatus and Sitophilus zeamais were tested. The dust had no significant effect on the two insects, none o... Insecticidal effects of different doses of the dust and methanol extracts of Garcinia kolae on Callosobruchus maculatus and Sitophilus zeamais were tested. The dust had no significant effect on the two insects, none of them died even at 3 d after treatment. The methanol extracts, however, had rapid lethal effects on both C. maculatus and S. zeamais. The mortality of C. maculatus by the lowest concentration of methanol extracts ranged from 95%-100% whereas in S. zeamais, the mortality ranged from 87.5%-100% and 70%-100% in concentrations of 1 g extract+3 ml methanol and 1 g extract+5 ml methanol, respectively, from 24 to 48 h. The least concentration of 1 g extract+l 5 ml methanol had no significant lethal effect on Sitophilus zeamais. 展开更多
关键词 Insecticidal effect Garcinia kolae Sitophilus zeamais Callosobruchus maculatus
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Insecticidal Activity of the Powders Obtained from the Bauxite Red Mud, Zyzygium aromaticum and Citrus sinensis Peel Extracts on the Conservation of Corn against Sitophilus zeamais: Physicochemical Properties
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作者 Noomte Yohana Yves Hambate Gomdje Valery +1 位作者 Djomou Djonga Paul Nestor Benoit Loura 《材料科学与工程(中英文B版)》 2021年第3期117-131,共15页
The remarkable push of technologies increasingly recommends materials for their constructions.Hence the extraction of alumina is for different uses.The exploitation of bauxite pollutes the surrounding areas in this ca... The remarkable push of technologies increasingly recommends materials for their constructions.Hence the extraction of alumina is for different uses.The exploitation of bauxite pollutes the surrounding areas in this case the discharge of mud wheel.Furthermore,the excessive use of dangerous conventional synthetic insecticides continues to be in circulation in Cameroon despite the known adverse effects.These problems lead us to formulate bio insecticides from rejection of the extraction of alumina and extracts of S.aromaticum and Citrus sinensis which respectively offer us essential oils eugenol and limonene having insecticidal properties against the main insects,pests of corn stocks,Sitophilus zeamais.From the kinetic point of view of the adsorption of the extracts of eugenol,limonene and their association by the red mud,the adsorption curve shows that the Eu/Li mixture(30/70)is better by 0.36 mg/g during the 24 h.The results showed a high toxicity of the Eu-Li binary mixture of ratio 30/70 with respective mortality rates of 95.88%after 8 days of exposure to the concentration of 5 mL/L on the adults of Sitophilus zeamais.The other composites induce an average rate of 80%.The results from the physicochemical analysis of corn before and after treatment with formulated insecticides showed a slight loss in nutritional quality of 14.90%in ash,12.5%in protein,16.14%in lipids and 13.63%in total sugars for 180 days.The powdery formulation from red mud and Eu-Li(30/70)mixture can therefore ensure the protection of corn stocks against Sitophilus zeamais in a farming environment. 展开更多
关键词 Insecticidal activity red mud Syzygium aromaticum Citrus sinensis Sitophilus zeamais physicochemical properties.
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Interspecific Competition and Grain-Hosts Selection of Maize Weevil, <i>Sitophilus zeamais</i>(Motsch.) (Coleoptera;Dryophtoridae) and Larger Grain Borer, <i>Prostephanus truncatus</i>(Horn) (Coleoptera;Bostrichidae)
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作者 Déthié Ngom Cheikh Thiaw MbackéSembène 《Advances in Entomology》 2020年第1期34-45,共12页
Laboratory interspecific competition and grain-hosts selection experiments involving maize weevil (MW), Sitophilus zeamais (Motsch.) and larger grain borer (LGB), Prostephanus truncatus (Horn) were performed under amb... Laboratory interspecific competition and grain-hosts selection experiments involving maize weevil (MW), Sitophilus zeamais (Motsch.) and larger grain borer (LGB), Prostephanus truncatus (Horn) were performed under ambient temperature and relative humidity (25°C - 35°C and 70% - 80% relative humidity) in their major host cereal, maize grains. The species reproductive rate and grain-hosts preference were evaluated by a number of emerged adults. In combined infestation, both species competed intensively by simultaneously increasing their individuals’ emergence, 9-fold or greater than when reared alone. Even though both species simultaneously increased their progeny, S. zeamais was the dominant competitor and had a significant suppressant effect on P. truncatus. The selection result of grain-hosts showed that P. truncatus placed in the environment containing both uninfested maize grains and grains previously infested by S. zeamais, the insect prefers uninfested grains. Indeed, P. truncatus individuals’ emergences were significantly more important to uninfested grains than to infested grains. The weevil-infested grains seemed to have deterrent and detrimental effects on P. truncatus. Conversely, S. zeamais selection of grain-hosts was significantly (P = 0.0001) more attracted by P. truncatus infested grains than to uninfested grains. Sitophilus zeamais individuals’ emergences were significantly (P = 0.0008) more important to infested grains than to uninfested grains. The S. zeamais preference on grains previously infested would be stimulated by P. truncatus larval vibrations in grain. 展开更多
关键词 Prostephanus truncatus Sitophilus zeamais Interspecific Competition Grain-Hosts SELECTION Deterrent Effect
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黄荆精油对玉米象的杀虫活性成分、毒力及作用机制 被引量:24
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作者 卢传兵 薛明 +2 位作者 刘雨晴 刘爱红 王洪涛 《昆虫学报》 CAS CSCD 北大核心 2009年第2期159-167,共9页
为明确黄荆精油及其主要成分作为储粮保护剂的开发利用价值,采用水蒸气蒸馏法从泰山黄荆Vitex,negundo Linn.叶片中提取精油,利用GC-MS技术进行了成分分析鉴定,研究了黄荆精油及其主要成分桉树脑和α-蒎烯对玉米象Sitophilus zeamais Mo... 为明确黄荆精油及其主要成分作为储粮保护剂的开发利用价值,采用水蒸气蒸馏法从泰山黄荆Vitex,negundo Linn.叶片中提取精油,利用GC-MS技术进行了成分分析鉴定,研究了黄荆精油及其主要成分桉树脑和α-蒎烯对玉米象Sitophilus zeamais Motschulsky成虫的毒力及作用机制。结果表明:黄荆精油中含量大于0.3%的成分有31种,其中石竹烯、桉树脑和α-蒎烯的含量分别为35.97%,8.21%和0.69%。桉树脑、α-蒎烯和黄荆精油对玉米象成虫的综合杀虫毒力都较高,并以桉树脑的毒力最高,LC_(50)为0.7171 g·kg^(-1);在2.0 g·kg^(-1)剂量下,桉树脑、α-蒎烯和黄荆精油对玉米象种群的抑制率分别达100%,85.45%和89.73%;3种药剂对玉米象72 h触杀LC_(50)分别为0.2690,0.7529和0.2969 mg·cm^(-2),毒力都很高;3种药剂在4g·kg^(-1)剂量下,对玉米象的驱避率分别为96.49%,84.26%和90.61%,防虫作用大;3种药剂对玉米象72 h的熏蒸LC_(50)分别为14.053,28.648和21.429μL·L^(-1),熏蒸杀虫毒力较高,其中以桉树脑的毒力最高。3种药剂对玉米象有干扰呼吸的作用,对离体乙酰胆碱酯酶、过氧化氢酶和羧酸酯酶都有抑制作用。这些结果明黄荆精油及其主要成分的作用机制具有多样性。 展开更多
关键词 玉米象 黄荆 精油 桉树脑 α-蒎烯 毒力 杀虫机理
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九种植物精油对玉米象成虫的熏蒸作用比较 被引量:34
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作者 邓永学 王进军 +1 位作者 鞠云美 张海燕 《农药学学报》 CAS CSCD 2004年第3期85-88,共4页
采用广口瓶密闭方法,测定红桔油Citrustangerina、柠檬叶油Citruslimonum、红河橙油Citrushongheensis、山苍子油Litseacubeba、留兰香油Menthaspicata、松节油Pinustabulaeformis、黄樟油Cinnamonumcamphora、互叶白千层油Melaleucaalt... 采用广口瓶密闭方法,测定红桔油Citrustangerina、柠檬叶油Citruslimonum、红河橙油Citrushongheensis、山苍子油Litseacubeba、留兰香油Menthaspicata、松节油Pinustabulaeformis、黄樟油Cinnamonumcamphora、互叶白千层油Melaleucaalternifolia、蓝桉叶油Eucalyptusglobulus等9种植物精油,在熏杀浓度为16μL/L、熏蒸时间为24、36、48及60h条件下,对玉米象Sitophiluszeamais成虫的熏蒸活性。结果表明:黄樟油、互叶白千层油、柠檬叶油、留兰香油及松节油的熏杀效果较好,尤以黄樟油的效果最佳。黄樟油在12、24、36、48和60h不同熏蒸时间下的LC50值分别为31.43、11.26、6.16、2.72和1.11μL/L。可考虑将黄樟油作为磷化氢及溴甲烷的替代药物用于控制玉米象的危害。 展开更多
关键词 植物精油 玉米象 熏蒸活性 黄樟油
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硝酸盐供应对玉米侧根生长的影响 被引量:37
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作者 郭亚芬 米国华 +1 位作者 陈范骏 张福锁 《植物生理与分子生物学学报》 CAS CSCD 北大核心 2005年第1期90-96,共7页
以两个玉米(Zea mays L.)自交系478和Wu312 为研究材料,采用琼脂培养方法,研究不同浓度NO3- 对侧根生长的影响。结果表明,在外部浓度0.01-1.0 mmol/L范围内,NO3-供应能显著增加侧根的长度及根 生物量。但当NO3-供应超过1.0 mmol/L后,... 以两个玉米(Zea mays L.)自交系478和Wu312 为研究材料,采用琼脂培养方法,研究不同浓度NO3- 对侧根生长的影响。结果表明,在外部浓度0.01-1.0 mmol/L范围内,NO3-供应能显著增加侧根的长度及根 生物量。但当NO3-供应超过1.0 mmol/L后,侧根长度 开始下降。当NO3-供应分别在超过5.0(Wu312)与10 (478)mmol/L后,侧根密度显著下降。在10 mmol/L NO3-下,Wu312的侧根生长几乎完全被抑制。而478在 20 mmol/L时,侧根密度仍可达到其最大值的30%(主 根)-50%(胚根)。植株地上部全氮及硝酸盐含量随 NO3-供应的增加而升高,二者与侧根长度、侧根密度 及冠根比的数学函数关系相同。 展开更多
关键词 NO3^- 侧根数量 侧根长度 氮状况 玉米(Zeamays L.)
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玉米穗行数QTL及其互作分析 被引量:5
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作者 李成璞 白苇 +2 位作者 翟立红 陶勇生 张祖新 《植物遗传资源学报》 CAS CSCD 北大核心 2011年第6期965-970,共6页
利用与穗行数有关的5个导入系及轮回亲本综3进行GriffingⅣ双列杂交发展分离群体,结合SSR标记和田间表型鉴定,分析玉米穗行数QTL及其相互作用。在导入系×综3所发展的5个F2群体中,仅在一个群体中检测到1个穗行数QTL,所解释的表型变... 利用与穗行数有关的5个导入系及轮回亲本综3进行GriffingⅣ双列杂交发展分离群体,结合SSR标记和田间表型鉴定,分析玉米穗行数QTL及其相互作用。在导入系×综3所发展的5个F2群体中,仅在一个群体中检测到1个穗行数QTL,所解释的表型变异为10.68%。在导入系间杂交所发展的F2群体中检测到9个QTLs,分别位于第13、、8染色体上,所解释的表型变异在4.53%~6.52%之间。另外,检测到2对QTL间互作,10对QTL与未检测到QTL的导入片段间的互作,单个F2群体中各类互作所解释的表型变异显著大于QTL所解释的表型变异。这些结果表明,基因互作在玉米穗行数形成中起着重要的作用。 展开更多
关键词 玉米(Zeamays L.) 穗行数 数量性状位点 遗传效应 上位性
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Effects of Interspecific Competition on the Population Dynamics of Four Stored Grain Insect Pests 被引量:3
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作者 崔晋波 邓永学 +3 位作者 王进军 杨龙德 杨自力 蒋天科 《Zoological Research》 CAS CSCD 北大核心 2006年第3期275-280,共6页
The effects of interspecific competition on the population dynamics of four insect grain Insecp pests ( Sitophilus zeamais, Rhizopertha dominica, Tribolium castaneum and Cryptolestes ferrugineus ) was studied at 30... The effects of interspecific competition on the population dynamics of four insect grain Insecp pests ( Sitophilus zeamais, Rhizopertha dominica, Tribolium castaneum and Cryptolestes ferrugineus ) was studied at 30℃, 75% relative humidity. A regression analysis of the population dynamics was conducted. The results showed that population growth of S. zeamais , R. dominica, T. castaneum and C. ferrugineus was restrained under mixed rearing. Population growth of T. castaneum and C. ferrugineus was remarkably accelerated when they coexisted with S. zeamais or R. dominica, while that of S. zeamais and R. dominica was restrained to some extent. Regression analysis results showed that the population growth potential of S. zeamais was the largest and that of C. ferrugineus was the smallest, and the changing trends of population growth rates were not distinct. 展开更多
关键词 Interspecific competition Population dynamics Sitophilus zeamais Rhizopertha dominica Tribolium castaneum Cryptolestes ferrugineus
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Insecticidal Activity of Crude Extract of Saponins isolated from Sapindus miukurossi Gatren 被引量:3
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作者 录丽平 孙长德 +3 位作者 杨美林 秦小萍 张玲 高晓余 《Plant Diseases and Pests》 CAS 2010年第3期55-56,62,共3页
[Objective] The study aimed to provide the theoretical basis for the development of Saponins isolated from Sapindus mukurossi in the application of pesticides and lay the foundation for the development and utilization... [Objective] The study aimed to provide the theoretical basis for the development of Saponins isolated from Sapindus mukurossi in the application of pesticides and lay the foundation for the development and utilization of Sapindus species resources in Yunnan province. [ Method] Saponine from S. mukurossi was extracted by the methods of dipping, feed mixed with pesticide, unlimited feeding, and conducted indoor toxicity determination on Plutella xylostella (Linnaeus), Sitophilus zeamais motschulsky and Drosophila. [ Result] Saponin had no poisoning activity on maize weevil, while it had certain biological activity on diamondback moth and fruit fly, but it had high activity on fruit fly, when the concentration was 50 mg/ml, mortality was 100% at 72 h, LCso was 4.06 mg/ml at 72 h, LTso was 30.19 h when the concentration was 25.000 mg/ml. [Conclusion] Saponins from S. mukurossi could be develooed and utilized as the material for botanical eesticide. 展开更多
关键词 Sapindus miukurossi gatren Plutella xylostella (Unnaeus) Sitophilus zeamais motschulsky DROSOPHILA Toxicity test
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盆栽与滩涂种植鉴定玉米耐盐性的比较分析 被引量:3
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作者 崔世友 薛林 +3 位作者 彭长俊 程玉静 冒宇翔 陈小晖 《长江大学学报(自科版)(中旬)》 CAS 2012年第4期1-5,14,共6页
选用12个玉米(Zeamays L.)品种(组合),分别进行盆栽土培苗期耐盐性鉴定和沿海滩涂耐盐性全生育期鉴定。盆栽鉴定每盆装土5kg,3叶后进行逆境处理(浇4次500ml 150mmol/L的NaC1);滩涂土壤含盐量为(3.8±0.5)‰,选择总生物量、苗长、叶... 选用12个玉米(Zeamays L.)品种(组合),分别进行盆栽土培苗期耐盐性鉴定和沿海滩涂耐盐性全生育期鉴定。盆栽鉴定每盆装土5kg,3叶后进行逆境处理(浇4次500ml 150mmol/L的NaC1);滩涂土壤含盐量为(3.8±0.5)‰,选择总生物量、苗长、叶片数、叶面积、盐害级别等5个苗期指标以及株高、籽粒产量等2个全生育期鉴定指标。通过盆栽苗期与滩涂全生育期鉴定结果的比较分析,研究盆栽苗期鉴定玉米耐盐性的可行性。结果表明,苏玉947、郑单958和TP1101等3个品种(组合)耐盐性强,而登海9号、苏玉19等2个品种对盐逆境很敏感。盐逆境对玉米苗总生物量、苗长、叶片数、叶面积均有极显著的降低效应,苗长可作为盆栽苗期鉴定的可靠指标。相关分析表明总生物量、苗长、叶片数与株高、籽粒产量间的相关系数均达显著水平,其余二个指标叶面积、盐害级别与滩涂鉴定指标间达到或接近显著水平。盆栽苗期鉴定与滩涂种植鉴定玉米耐盐性的一致性较好,可通过盆栽土培方法进行大规模的玉米种质或品种耐盐性鉴定。 展开更多
关键词 玉米(Zeamays L.) 耐盐性 盆栽鉴定 滩涂鉴定
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种植密度对不同玉米品种抗倒伏性及产量的影响 被引量:28
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作者 张洪生 盖伟玲 +1 位作者 李玲燕 姜雯 《中国种业》 2009年第6期34-35,共2页
以不同高产玉米品种为试验材料,研究种植密度对玉米抗倒伏性、产量影响以及品种间差异。结果表明:玉米茎秆穿刺强度随密度增大显著减弱,即高密度下植株抗倒伏能力显著减弱,而品种间穿刺强度存在显著差异,其中登海3719在低、中密度下各... 以不同高产玉米品种为试验材料,研究种植密度对玉米抗倒伏性、产量影响以及品种间差异。结果表明:玉米茎秆穿刺强度随密度增大显著减弱,即高密度下植株抗倒伏能力显著减弱,而品种间穿刺强度存在显著差异,其中登海3719在低、中密度下各节间茎秆穿刺强度均显著大于其他两品种,抗倒伏性最强;各品种产量受种植密度影响显著,金海5号产量随密度增加而提高,而登海3719和郑单958产量均在中密度下达到最高,高密度下显著下降,金海5号产量在各密度下均高于其他两品种。 展开更多
关键词 玉米(Zeamays L.) 穿刺强度 产量
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Ca(OH)_2对酸性土壤玉米生长及土壤养分的影响 被引量:3
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作者 王孟 张军强 +7 位作者 姜汉峰 商水根 黄登武 黄飞跃 徐印印 姜硕琛 邢丹英 向永生 《湖北农业科学》 2019年第9期58-60,68,共4页
对紫色页岩发育的土壤(pH 5.2)施用Ca(OH)_2,探讨施用量对玉米(Zea mays L.)生长及土壤的影响。结果表明,施用Ca(OH)_2对酸化土壤的玉米生产有积极效果。当Ca(OH)_2施用量为0、750、1 500、2 250、3 000 kg/hm^2时,玉米产量分别为7 134.... 对紫色页岩发育的土壤(pH 5.2)施用Ca(OH)_2,探讨施用量对玉米(Zea mays L.)生长及土壤的影响。结果表明,施用Ca(OH)_2对酸化土壤的玉米生产有积极效果。当Ca(OH)_2施用量为0、750、1 500、2 250、3 000 kg/hm^2时,玉米产量分别为7 134.9、7 296.9、7 533.9、7 371.9、7 273.8 kg/hm^2,两者呈二次曲线(y=-0.000 1x^2+0.397 2x+7 120.4),方程达显著水平(R2=0.852 9,P<0.05);经计算,当Ca(OH)_2施用量为1 986kg/hm^2时,产量可达到最高值(8 303.66 kg/hm^2)。施用Ca(OH)_2能够提高酸化土壤的pH,施用量与土壤pH呈线性关系(y=0.009 7x+5.962 0),方程达极显著水平(R2=0.953 4,P<0.01),施用量为1 500 kg/hm^2时,土壤呈中性。施用Ca(OH)_2对土壤养分含量也有一定影响,Ca(OH)_2施用量与碱解氮含量呈三次曲线(y=-8×10^(-9)x^3+3×10^(-5)x^2-0.025x+111.87),方程达显著水平(R^2=0.914 4,P<0.05);Ca(OH)_2施用量与速效钾含量呈二次曲线(y=6×10^(-6)x^2-0.023 1x+73.257),方程达极显著水平(R^2=0.976 1,P<0.01);Ca(OH)_2施用量与有机质含量呈二次曲线(y=-4×10^(-7)x^2+0.000 2x+29.471),方程达显著水平(R^2=0.918 1,P<0.05)。 展开更多
关键词 CA(OH)2 酸化土壤 玉米(Zeamays L.) 土壤pH 土壤养分
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玉米幼苗对盐胁迫的响应和适应 被引量:2
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作者 罗辉 《井冈山大学学报(社会科学版)》 2004年第5期23-28,共6页
分别用不同浓度(0,50,75,100mmol/L雪的NaCl溶液处理三叶期玉米幼苗1d和7d,测定其鲜重、干重、含水重、无机离子和有机溶质,渗透调节能力等,结果显示:玉米幼苗生长受到盐的抑制,盐胁迫下,玉米幼苗吸收一定量的Na+、Cl-,主要渗透调节均... 分别用不同浓度(0,50,75,100mmol/L雪的NaCl溶液处理三叶期玉米幼苗1d和7d,测定其鲜重、干重、含水重、无机离子和有机溶质,渗透调节能力等,结果显示:玉米幼苗生长受到盐的抑制,盐胁迫下,玉米幼苗吸收一定量的Na+、Cl-,主要渗透调节均质是可溶性糖、游离氨基酸和有机酸等有机溶质,以有机渗透调节为主.处理7d后的渗透调节的效果更明显,且玉米地上部有拒盐作用. 展开更多
关键词 玉米(Zeamays) 盐胁迫 渗透调节 有机溶质
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一个玉米逆境响应基因ZmSPK1表达载体的构建及拟南芥转化
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作者 邹华文 李翠花 吴启侠 《长江大学学报(自科版)(中旬)》 CAS 2010年第2期49-52,共4页
首次在玉米(Zea mays L.)中克隆了1个SnRK家族基因,命名为ZmSPK1。为了研究ZmSPK1的功能,把ZmSPK1的cDNA连接到植物表达载体pGreen0029中,通过农杆菌介导转入拟南芥(Arabidop-sis)中进行过量表达。PCR及Western-blot分析表明,ZmSPK1已... 首次在玉米(Zea mays L.)中克隆了1个SnRK家族基因,命名为ZmSPK1。为了研究ZmSPK1的功能,把ZmSPK1的cDNA连接到植物表达载体pGreen0029中,通过农杆菌介导转入拟南芥(Arabidop-sis)中进行过量表达。PCR及Western-blot分析表明,ZmSPK1已经转入到拟南芥中,并且在大多数转化植株中稳定表达。 展开更多
关键词 玉米(Zeamays L.) SnRK ZmSPK1 拟南芥(Arabidopsis)
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车前草提取物对三种储粮害虫的触杀活性研究 被引量:5
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作者 梁飞 肖云丽 +1 位作者 徐艳霞 钟玉林 《湖北农业科学》 北大核心 2013年第22期5493-5495,共3页
采用冷浸法以4种不同极性的溶剂(乙醇、丙酮、乙酸乙酯和石油醚)对车前草(Plantago asiatica L.)活性成分进行粗提,采用药膜法研究提取物对玉米象(Sitophilus zeamais Motschulsky)、谷蠹(Tribolium castaneum)、赤拟谷盗(Rhyzopertha d... 采用冷浸法以4种不同极性的溶剂(乙醇、丙酮、乙酸乙酯和石油醚)对车前草(Plantago asiatica L.)活性成分进行粗提,采用药膜法研究提取物对玉米象(Sitophilus zeamais Motschulsky)、谷蠹(Tribolium castaneum)、赤拟谷盗(Rhyzopertha dominica Fbricius)3种储粮害虫成虫的触杀作用。结果表明,丙酮提取物、石油醚提取物和乙醇提取物分别对玉米象、谷蠹和赤拟谷盗触杀效果较好,试虫校正死亡率可达到97%以上。 展开更多
关键词 车前草(Plantago asiatica L )提取物 触杀活性 玉米象(Sitophilus zeamais Motschulsky) 赤拟谷盗(Rhyzopertha DOMINICA Fbricius) 谷蠹(Tribolium castaneum)
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改进FAO双作物系数法估算夏玉米蒸散量 被引量:2
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作者 肖然 朱永华 吕海深 《湖北农业科学》 2019年第9期33-36,共4页
为了更加准确地估算淮北平原夏玉米(Zea mays L.)蒸散量,基于安徽省蚌埠市五道沟水文试验站实测的叶面积指数数据,对FAO双作物系数法中的基础作物系数(K_(cb))和土面蒸发系数(K_e)的计算方法进行改进;并以2016、2017两年基于蒸渗仪实测... 为了更加准确地估算淮北平原夏玉米(Zea mays L.)蒸散量,基于安徽省蚌埠市五道沟水文试验站实测的叶面积指数数据,对FAO双作物系数法中的基础作物系数(K_(cb))和土面蒸发系数(K_e)的计算方法进行改进;并以2016、2017两年基于蒸渗仪实测的夏玉米蒸散量对FAO双作物系数法和改进双作物系数法的估算结果的准确性进行评估。结果表明,改进双作物系数法在夏玉米各生长阶段及全生育期的估算结果准确性都高于FAO双作物系数法;改进双作物系数法在夏玉米拔节期至抽雄期的蒸散量计算中改进效果最为明显。说明改进双作物系数法更适用于淮北平原夏玉米蒸散量的估算。 展开更多
关键词 夏玉米(Zeamays L.) 叶面积指数 作物系数法 作物蒸散量 淮北平原
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Contact and fumigant toxicities of calamusenone isolated from Acorus gramineus rhizome against adults of Sitophilus zeamais and Rhizopertha dominica 被引量:3
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作者 Yan-Zhang Huang Hong-Xia Hua +1 位作者 Shi-Guana Li Chang-Ju Yang 《Insect Science》 SCIE CAS CSCD 2011年第2期181-188,共8页
Calamusenone [3,8-dimethyl-5-(1-methylethylidene)- 1,2,3,4,5,6,7,8-octa- hydroazulene-6-one, C15H22O] from Acorus gramineus Soland rhizome was tested in the laboratory for its insecticidal activities against adults ... Calamusenone [3,8-dimethyl-5-(1-methylethylidene)- 1,2,3,4,5,6,7,8-octa- hydroazulene-6-one, C15H22O] from Acorus gramineus Soland rhizome was tested in the laboratory for its insecticidal activities against adults of Sitophilus zeamais Motsch. and Rhizopertha dominica (Fab.), using dry film contact and fumigation methods. Responses varied with insect species, dosage and exposure time. In the dry film contact experiment, the highest insecticidal effects of calamusenone against S. zeamais and R. dominica adults were produced at 170.32μg/cm2 after treatment for 72 h, with 96.2% and 98.7% mortalities, respectively. The median lethal concentration (LC50) (72 h) values of calamusenone against S. zeamais and R. dominica adults were 67.00 μg/cm2 and 77.30 μg/cm2, respectively. As a potential fumigant, calamusenone showed moderate insecticidal effect on the adults of S. zeamais and R. dominica in fumigation experiment, with their LC50 (120 h) values of 125.71μL/L and 93.64μL/L respectively. Calamusenone isolated from A. gramineus rhizome showed promise as a novel pesticide candidate for stored-product pest control. 展开更多
关键词 Acorus gramineus Soland calamusenone INSECTICIDE Rhizopertha dominica(Fab.) Sitophilus zeamais Motsch.
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植物CytP450和抗氧化酶对土壤菲、芘暴露的生态毒理响应 被引量:9
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作者 宋玉芳 王磊 +5 位作者 李昕馨 张薇 陈朗 Freestone David 吉普辉 Ackland M Leigh 《环境科学学报》 CAS CSCD 北大核心 2009年第2期381-388,共8页
以玉米(ZeamaysL.)为供试植物,草甸棕壤为供试土壤,以微粒体细胞色素P450含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性为指标,进行了土壤菲、芘暴露的生态毒理响应研究.结果表明,菲、芘暴露均能引起植物代谢解毒... 以玉米(ZeamaysL.)为供试植物,草甸棕壤为供试土壤,以微粒体细胞色素P450含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性为指标,进行了土壤菲、芘暴露的生态毒理响应研究.结果表明,菲、芘暴露均能引起植物代谢解毒和抗氧化防御系统的胁迫响应,不同程度引发植物代谢解毒及抗氧化能力的改变.P450酶活性与低浓度菲、芘单一暴露浓度具有相关性(r=0.834,p<0.01),与菲、芘复合暴露浓度负相关,说明菲、芘复合暴露导致代谢解毒能力下降,对植物的代谢解毒具有协同毒性效应;SOD酶活性与菲、芘单一暴露浓度负相关,CAT酶活性与菲、芘单一暴露浓度正相关,POD酶活性与菲的水溶解度正相关,而与芘的总浓度负相关.SOD、CAT和POD酶活性与菲、芘复合暴露浓度均呈正相关,说明菲、芘复合暴露导致氧化损伤程度减弱,对植物的氧化损伤具有拮抗作用. 展开更多
关键词 土壤 玉米(Zeamays L.) CytP450 抗氧化酶系
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