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Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells
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作者 Jeonghun Baek Chun Wai Mai +1 位作者 Wei Meng Lim Lai Chun Wong 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2023年第1期40-46,共7页
AIM:To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial(ARPE)-19 cells in vitro.METHODS:ARPE-19 cells were cultured in Dulbecco’s Modified Eagle Medium-F... AIM:To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial(ARPE)-19 cells in vitro.METHODS:ARPE-19 cells were cultured in Dulbecco’s Modified Eagle Medium-F-12 supplemented with 10%foetal bovine serum and 1%penicillin–streptomycin in a humidified 5%CO_(2) incubator maintained at 37℃.Cells were treated with 247μmol/L lutein,49μmol/L zeaxanthin and 1%(v/v)of either coconut oil,corn oil,peanut oil,olive oil,sunflower oil,soybean oil,castor oil,or linseed oil for 48h.Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography.RESULTS:Among the oils tested,the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil.CONCLUSION:ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils. 展开更多
关键词 LUTEIN zeaxanthin adult retinal pigment epithelial-19 cells vegetable oil
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Research Progress on Purification Process Optimization and Content Determination of Zeaxanthin
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作者 Jian LIU Jinglong CAO +3 位作者 Hui XUE Yannan LI Wenshuang HOU Chenghao JIN 《Asian Agricultural Research》 2023年第3期37-39,46,共4页
At present,the purification process of zeaxanthin mainly includes organic solvent extraction,ultrasonic-assisted extraction and enzyme extraction,and the content determination technology mainly includes ultraviolet-sp... At present,the purification process of zeaxanthin mainly includes organic solvent extraction,ultrasonic-assisted extraction and enzyme extraction,and the content determination technology mainly includes ultraviolet-spectrophotometry and high performance liquid chromatography.In this paper,the purification process and content determination technology of zeaxanthin in recent years are reviewed in order to provide ideas and theoretical basis for further research and application of zeaxanthin. 展开更多
关键词 zeaxanthin CAROTENOID Purification process Content determination
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Application of Lutein and Zeaxanthin in nonproliferative diabetic retinopathy 被引量:4
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作者 Bo-Jie Hu Ya-Nan Hu +2 位作者 Song Lin Wen-Jiang Ma and Xiao-Rong Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2011年第3期303-306,共4页
AIM: To compare serum Lutein and Zeaxanthin (L/Z) concentrations between patients with nonproliferative diabetic retinopathy (NDR) and normal subjects,and to explore the effect of L/Z supplementation on serum L/Z leve... AIM: To compare serum Lutein and Zeaxanthin (L/Z) concentrations between patients with nonproliferative diabetic retinopathy (NDR) and normal subjects,and to explore the effect of L/Z supplementation on serum L/Z level and visual function in NDR patients METHODS: Subjects were divided into three groups: 30 NDR patients supplied with Lutein 6mg/d and Zeaxanthin 0.5mg/d for three months (DR Group),30 NDR patients without L/Z supplementation (DR Control Group) and 30 normal subjects (Control Group).Serum L/Z concentrations were measured by liquid high-resolution chromatography (HPLC).Visual acuity was recorded at baseline,1 month,2 months and 3 months post initial supplementation.Serum L/Z concentration were measured at baseline,1 month and 2 months post initial supplementation.Contrast sensitivity (CS) and fovea thickness were recorded at baseline and 3 months post initial supplementation.RESULTS: Mean serum lutein concentrations in DR group were 0.0686±0.0296μg/mL and zeaxanthin concentration was 0.0137±0.0059μg/mL.The L/Z level of DR group was significantly lower compared to the control group( lutein: 0.2302±0.1308μg/mL,zeaxanthin: 0.0456±0.0266μg/m,P = 0.000).The concentration of lutein and zeaxanthin in the DR control group at base line was 0.0714±0.0357μg/mL and 0.0119±0.0072μg/mL,respectively.There was no significant change of L/Z concentration in the DR control group during the study.Serum L/Z concentrations of DR group increased significantly after supplementation(F=109.124,P=0.000;F=219.207,P=0.000).Visual acuity improved significantly after medication.Compared with pre-medication,the average CS values of 1.5cpd,3cpd and 6cpd after three months increased significantly (P =0.030,0.013,0.008) and the foveal thickness decreased.(P =0.05) CONCLUSION: Serum L/Z concentrations in DR patients are significantly lower than those in normal subjects,and L/Z intake can improve the visual acuity,CS and macular edema in DR patients,suggesting that L/Z supplementation might be targeted as potential potential therapeutic agents in treating NDR. 展开更多
关键词 LUTEIN zeaxanthin liquid high-resolution chromatography contrast sensitivity optical coherence tomography visual acuity nonproliferative diabetic retinopathy
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Effect of zeaxanthin on porcine embryonic development during in vitro maturation 被引量:1
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作者 Seo-jin Park Kyoung-Ha So Sang-Hwan Hyun 《The Journal of Biomedical Research》 CAS CSCD 2017年第2期154-161,共8页
Zeaxanthin is a common carotenoid, which is a powerful antioxidant that protects against damage caused by reactive oxygen species. The aim of the present study was to investigate the effects of zeaxanthin supplementat... Zeaxanthin is a common carotenoid, which is a powerful antioxidant that protects against damage caused by reactive oxygen species. The aim of the present study was to investigate the effects of zeaxanthin supplementation on in vitro maturation of porcine embryo development. We investigated nuclear maturation, intracellular glutathione (GSH), and reactive oxygen species (ROS) levels during in vitro maturation, and subsequent embryonic development following parthenogenetic activation and in vitro fertilization OVF). The oocytes were maturated and used at the metaphase II stage. After 42 hours of in vitro maturation, the zeaxanthin-treated group (0.5 μmol/L) showed significant increases in nuclear maturation (89.6%) than the control group (83.4%) (P 〈 0.05). The intracellular GSH levels increased significantly (P 〈 0.05) as zeaxanthin concentrations increased; ROS generation levels decreased with increased zeaxanthin concentrations, but there were no significant differences. There were no significant differences in subsequent embryonic development, cleavage rate, blastocyst stage rate, and total blastocyst cell numbers following parthenogenetic activation and IVF when in vitro maturation media was supplemented with zeaxanthin. These results suggest that treatment with zeaxanthin during in vitro maturation improved the nuclear maturation of porcine oocytes by increasing the intracellular GSH level, thereby slightly decreasing the intracellular ROS level. 展开更多
关键词 in vitro maturation PORCINE embryonic development zeaxanthin ANTIOXIDANT
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Meso-Zeaxanthin:One of Important Carotenoids for Human Health 被引量:1
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作者 XU Xinde JIANG Lianzhou +1 位作者 ZHANG Lihua SHAO Bin 《Journal of Northeast Agricultural University(English Edition)》 CAS 2010年第3期76-81,共6页
As one of optical isomers of zeaxanthin, meso-zeaxanthin is an important ingredient of macular pigment. Compared with lutein and zeaxanthin, meso-zeaxanthin may display strong functions as antioxidant and filtering sh... As one of optical isomers of zeaxanthin, meso-zeaxanthin is an important ingredient of macular pigment. Compared with lutein and zeaxanthin, meso-zeaxanthin may display strong functions as antioxidant and filtering short-wavelength, for example, the blue light. Comparisons among lutein, zeaxanthin and meso-zeaxanthin as well as functions and distributions together with the source of meso-zeaxanthin were discussed. The application as a supplement of diet and issues should be studied in the future for mesozeaxanthin were also analyzed. 展开更多
关键词 meso-zeaxanthin FUNCTION distribution SOURCE application
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The protective effect of zeaxanthin on human limbal and conjunctival epithelial cells against UV-induced cell death and oxidative stress
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作者 Yue Huang Chun Shi Jing Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2019年第3期369-374,共6页
AIM: To explore the protective effect of zeaxanthin on human limbal and conjunctival epithelial cells against UVradiation and excessive oxidative stress.METHODS: Human limbal and conjunctival epithelial cells were iso... AIM: To explore the protective effect of zeaxanthin on human limbal and conjunctival epithelial cells against UVradiation and excessive oxidative stress.METHODS: Human limbal and conjunctival epithelial cells were isolated from cadaver and cultured in vitro. They were challenged with UVB radiation and H2 O2 with and without zeaxanthin pretreatment. Cell viability, p38 and c-JUN NH(2)-terminal kinase(JNK) phosphorylation, IL-6, IL-8 and MCP-1 secretion and malondialdehyde(MDA) content were measured.RESULTS: Zeaxanthin had no measurable cytotoxicity on limbal or conjunctival epithelial cells when used at concentrations of 5 μg/mL and below. At 30 mJ/cm2 UVB, the pretreatment of zeaxanthin increased the percentage of live cells from 50% to 69%(P=0.01) and from 66% to 75%(P=0.05) for limbal and conjunctival epithelial cells, respectively. The concentrations of IL-6, IL-8 and MCP-1 in the culture medium reduced to 66%(for IL-6 and MCP-1)and 56%(for IL-8) of the levels without zeaxanthin. This was accompanied by reduced p38 and JNK protein phosphorylation. Pretreatment of zeaxanthin also reduced intracellular MDA content caused by H2 O2 stimulation from 0.86 μmol/L to 0.52 μmol/L(P=0.02) in limbal epithelial cells and from 0.96 μmol/L to 0.56 μmol/L in conjunctival epithelial cells(P=0.03). However, zeaxanthin did nothave significant effect on H2 O2-induced cell death in limbal or conjunctival epithelial cells.CONCLUSION: Zeaxanthin is an effective reagent in reducing the detrimental effect of UV-radiation and oxidative stress on ocular surface epithelial cells. 展开更多
关键词 zeaxanthin UV-radiation oxidative stress MALONDIALDEHYDE limbal EPITHELIAL CELLS CONJUNCTIVAL EPITHELIAL CELLS
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Macular pigment and serum zeaxanthin levels with Goji berry supplement in early age-related macular degeneration
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作者 Shang Li Na Liu +3 位作者 Li Lin Er-Dan Sun Jian-Da Li Peng-Kun Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第6期970-975,共6页
AIM: To evaluate the efficacy of Goji berry supplementation on improving macular pigment, serum zeaxanthin levels and visual acuity in patients with early age-related macular degeneration (AMD). METHODS: A total ... AIM: To evaluate the efficacy of Goji berry supplementation on improving macular pigment, serum zeaxanthin levels and visual acuity in patients with early age-related macular degeneration (AMD). METHODS: A total of 114 patients (aged from 51 to 92y, mean age 69.53±8.41y) with early AMD were enrolled in our prospective, randomized controlled study. The included patients were assigned randomly to the Goji group (n=57) with 25 g of Goji berries supplementation per day for 90d and the control group (n=57) with their normal diet for 90d. Macular pigment optical density (MPOD) was measured using heterochromatic flicker photometry (HFP). The levels of serum lutein (L)/zeaxanthin (Z) were analyzed using high-performance liquid chromatography (HPLC). MPOD, serum L/Z levels and best corrected visual acuity (BCVA) were recorded at baseline and 90d. RESULTS: In the Goji group, there were no statistically significant differences in the serum L levels between the baseline (0.199±0.149 μmol/mL) and 90d (0.203±0.181 μmol/mL) (t=-0.186, P=0.850); however the serum Z levels were increased at 90d (0.101±0.087 μmol/mL) compared with those at the baseline (0.029±0.032 μmol/mL) (t=6.412, P〈0.001). Patients treated with Goji berry for 90d showed an elevated MPOD (0.877±0.202 DU) from the baseline (0.731±0.205 DU) (t=-4.741, P=0.000). In contrast to the control group, the serum Z levels and MPOD were higher in the Goji group at 90d (both P〈0.05). At 90d, patients with Goji berry supplementation had a relative decrease in BCVA (0.21±0.18 logMAR) compared with the baseline (0.27±0.20) (t=2.397, P=0.020). CONCLUSION: Overall, daily supplementation with Goji berry for 90d improves MPOD by increasing serum Z levels rather than serum L levels in early AMD patients. Goji berry may be an effective therapeutic intervention for preventing the progression of early AMD. 展开更多
关键词 Goji berry zeaxanthin early age-relatedmacular degeneration
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Optimization of Zeaxanthin Production by Immobilized <i>Flavobacterium</i>sp. Cells in Fluidized Bed Bioreactor
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作者 Ma Del Carmen Chavez-Parga Alejandro Munguia-Franco +1 位作者 Mayanin Aguilar-Torres Eleazar M. Escamilla-Silva 《Advances in Microbiology》 2012年第4期598-604,共7页
From time immemorial, human beings have used pigments made from vegetables, fruits, superior plants, animal tissues and cereals. One of the greatest sources of pigments is the bacterium that, with the use of the moder... From time immemorial, human beings have used pigments made from vegetables, fruits, superior plants, animal tissues and cereals. One of the greatest sources of pigments is the bacterium that, with the use of the modern technology, has increased the production of metabolites of interest. The microbiological production of carotenoids has not been optimized to obtain pigment production quantities of pigments and carotenoids recovery that lower production costs. The aim of this work was to design a Zeaxanthin production process with Flavobacterium sp. immobilized cells in a fluidized bed bioreactor. An optimum culture medium for Zeaxanthin production in stirred flasks (2.46 g·L–1) was obtained. Furthermore, optimum process conditions for a maximum yield of Zeaxanthin production, by fluidized bed bioreactor, were established. A statistical analysis showed that the most significant factors were air flow, pH and NaCl concentration (4.5 g·L–1). In this study a maximum Zeaxanthin production of 3.8 g·L–1 was reached. The highest reported yield to date was 0.329 g·L–1. 展开更多
关键词 Fluidized Bed BIOREACTOR Orthogonal Design FLAVOBACTERIUM sp. zeaxanthin
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Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat
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作者 Chao-Yan Chang Shu-Xian Yang +14 位作者 Mei-Qi Zhang Yue-Ting Guo Xiao-Ming Li Yan Yan Ci-Hang Ding Ke-Xin Niu Meng-Lu Wang Qin-Quan Li Junli Zhang Xuebin Zhang Shisheng Chen Chaojie Xie Zhongfu Ni Qixin Sun Jin-Ying Gou 《Plant Communications》 SCIE CSCD 2023年第5期295-308,共14页
Reducing losses caused by pathogens is an effective strategy for stabilizing crop yields.Daunting challenges remain in cloning and characterizing genes that inhibit stripe rust,a devastating disease of wheat(Triticum ... Reducing losses caused by pathogens is an effective strategy for stabilizing crop yields.Daunting challenges remain in cloning and characterizing genes that inhibit stripe rust,a devastating disease of wheat(Triticum aestivum)caused by Puccinia striiformis f.sp.tritici(Pst).We found that suppression of wheat zeaxanthin epoxidase 1(ZEP1)increased wheat defense against Pst.We isolated the yellow rust slower 1(yrs1)mutant of tetraploid wheat in which a premature stop mutation in ZEP1-B underpins the phenotype.Genetic analyses revealed increased H_(2)O_(2) accumulation in zep1 mutants and demonstrated a correlation between ZEP1 dysfunction and slower Pst growth in wheat.Moreover,wheat kinase START 1.1(WKS1.1,Yr36)bound,phosphorylated,and suppressed the biochemical activity of ZEP1.A rare natural allele in the hexaploid wheat ZEP1-B promoter reduced its transcription and Pst growth.Our study thus identified a novel suppressor of Pst,characterized its mechanism of action,and revealed beneficial variants for wheat disease control.This work opens the door to stacking wheat ZEP1 variants with other known Pst resistance genes in future breeding programs to enhance wheat tolerance to pathogens. 展开更多
关键词 WHEAT stripe rust reactive oxygen species zeaxanthin epoxidase
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富含玉米黄质色素橄榄油延缓D-半乳糖诱导的小鼠衰老作用
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作者 叶文斌 陈文东 +3 位作者 王昱 何九军 黄新异 邸多隆 《现代食品科技》 CAS 北大核心 2024年第6期66-73,共8页
该文研究了富含玉米黄质色素橄榄油(Olive Oil Rich Zeaxanthin,OZT)对衰老小鼠的影响。将亚急性衰老模型造模成功的小鼠分为模型组、OZT高中低处理组、橄榄油组(Olive Oil,OL)、维生素E阳性(Vitamin E,VE)对照组,以生理盐水为CK(0)组... 该文研究了富含玉米黄质色素橄榄油(Olive Oil Rich Zeaxanthin,OZT)对衰老小鼠的影响。将亚急性衰老模型造模成功的小鼠分为模型组、OZT高中低处理组、橄榄油组(Olive Oil,OL)、维生素E阳性(Vitamin E,VE)对照组,以生理盐水为CK(0)组。连续灌胃28 d后,分析不同剂量OZT对衰老小鼠的体重、脾脏和胸腺指数、耐缺氧和抗疲劳作用、血清中SOD(Superoxide Dismutase)、CAT(Catalase)、GSH-Px(Glutathione Peroxidase)、ALT(Alanine Aminotransferase)、AST(Aspartate Aminotransferase)活性和MDA(Malondialdehyde)、IL-2(Interleukin-2)、IL-4、IL-10、IFN-γ(Interferon-γ)生成水平等指标的影响。结果显示,建模灌喂给药28 d后,Model组体质量与CK组小鼠相比存在极显著差异(P<0.01),而灌胃OZT的高中低剂量小鼠的体质量之间均存在显著差异(P<0.05);灌胃后OL组小鼠的耐缺氧时间和游泳时间最短,而OZT的高剂量组的小鼠时间最长,与VE组相比无显著差异(P>0.05);OZT还能降低衰老小鼠血清中的ALT、AST、SOD、CAT和GSH-Px的活性,提高IL-2、IL-4、IL-10和IFN-γ生成,降低MDA含量,且OZT高剂量组效果显著优于OL组,与VE接近。综上,OZT对D-半乳糖致亚急性衰老动物模型有很好的干预效果,能有效提高小鼠机体抗氧化能力,延长耐缺氧时间和提高抗疲劳作用,延缓衰老的作用。 展开更多
关键词 玉米黄质色素 橄榄油 衰老小鼠 影响
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葵花籽油微乳体系对玉米黄素增溶性能研究
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作者 吴红艳 王可意 +2 位作者 于晓丹 杨佳萌 田英华 《中国调味品》 CAS 北大核心 2024年第11期48-51,70,共5页
文章研究了葵花籽油微乳包封玉米黄素的能力,考察了玉米黄素在微乳不同相中的分配系数及葵花籽油微乳增溶玉米黄素的能力。结果表明,微乳的pH值在4~6之间时,体系稳定;pH值在2~4和8~10时,粒子分布不均匀,包封率降低。随着盐浓度的增加,... 文章研究了葵花籽油微乳包封玉米黄素的能力,考察了玉米黄素在微乳不同相中的分配系数及葵花籽油微乳增溶玉米黄素的能力。结果表明,微乳的pH值在4~6之间时,体系稳定;pH值在2~4和8~10时,粒子分布不均匀,包封率降低。随着盐浓度的增加,微乳的粒径和PDI增大,包封率逐渐下降。微乳在4℃和25℃下储存时较稳定并且粒径、PDI和包封率无明显改变;在45℃时,粒径较大且分布不均匀,包封率降低。微乳体系中玉米黄素的溶解度高于葵花籽油中玉米黄素的溶解度;在水包油型中,80%含水量的微乳增溶玉米黄素52倍,是水增溶玉米黄素的330倍。理论上可以确定有64个混合表面活性剂分子增溶1个玉米黄素分子。因此,葵花籽油微乳体系对玉米黄素具有良好的增溶性能。 展开更多
关键词 玉米黄素 微乳 葵花籽油 增溶性能
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玉米黄素减轻脂多糖诱导的3T3-L1脂肪细胞慢性炎症
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作者 赵有伟 吴熙 +5 位作者 朱晨 刘美宏 郑明珠 蔡丹 刘回民 刘景圣 《中国食品学报》 EI CAS CSCD 北大核心 2024年第7期47-59,共13页
目的:探究一种天然类胡萝卜素——玉米黄素(ZEA),基于NF-κB信号通路减轻脂多糖(LPS)诱导的3T3-L1脂肪细胞慢性炎症并发挥抗炎的作用机制。方法:以LPS刺激成熟的3T3-L1脂肪细胞建立炎症模型,用不同浓度的ZEA(0,5,10,15μmol/L)进行干预... 目的:探究一种天然类胡萝卜素——玉米黄素(ZEA),基于NF-κB信号通路减轻脂多糖(LPS)诱导的3T3-L1脂肪细胞慢性炎症并发挥抗炎的作用机制。方法:以LPS刺激成熟的3T3-L1脂肪细胞建立炎症模型,用不同浓度的ZEA(0,5,10,15μmol/L)进行干预,检测脂肪细胞炎症相关因子的含量,并通过实时荧光定量PCR(qPCR)检测其mRNA表达水平,用蛋白免疫印迹法检测信号通路因子的蛋白表达水平。结果:与LPS组相比,ZEA干预显著抑制炎症细胞模型中促炎因子IL-1β、IL-6、TNF-α和MCP-1分泌(P<0.001),同时促进抗炎因子IL-10和ADPN产生(P<0.001)。此外,qPCR结果显示ZEA能有效抑制促炎因子,同时恢复抗炎因子的mRNA表达水平(P<0.001)。蛋白免疫印迹结果表明,ZEA能明显降低IκB激酶催化亚基IKKα和IKKβ的蛋白和磷酸化表达(P<0.001),增强抑制蛋白IκBα与核转录因子NF-κB p65的结合,最终抑制NF-κB p65进行核内转录。结论:ZEA可通过抑制NF-κB信号通路激活及调控炎性因子的分泌,改善肥胖引起的慢性炎症。 展开更多
关键词 玉米黄素 脂多糖 慢性炎症 NF-ΚB信号通路 抗炎作用
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富含羟基酪醇和玉米黄质色素橄榄油对衰老小鼠影响的比较
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作者 叶文斌 陈文东 +3 位作者 王昱 何九军 黄新异 邸多隆 《甘肃农业大学学报》 CAS CSCD 北大核心 2024年第3期10-16,30,共8页
【目的】研究富含羟基酪醇橄榄油(olive oil rich in hydroxyl tyrosol,OHT)和富含玉米黄质色素橄榄油(olive oil rich in zeaxanthin,OZT)对衰老小鼠的影响。【方法】利用0.5 mL/10g剂量颈部皮下注射5%的D-半乳糖(D-galactose)溶液42 ... 【目的】研究富含羟基酪醇橄榄油(olive oil rich in hydroxyl tyrosol,OHT)和富含玉米黄质色素橄榄油(olive oil rich in zeaxanthin,OZT)对衰老小鼠的影响。【方法】利用0.5 mL/10g剂量颈部皮下注射5%的D-半乳糖(D-galactose)溶液42 d构建亚急性衰老模型,建模成功后,将小鼠随机分成5组,分别为模型组(Model group)、维生素E对照组(VE group)、普通橄榄油组(olive oil group,OL)、OHT组和OZT组,以生理盐水对照组CK(0)。连续灌胃28 d后,检测小鼠体质量、血清中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬酸氨基转移酶(aspartate aminotransferase,AST)活性和丙二醛(malondialdehyde,MDA)、白细胞介素-2(IL-2)、和干扰素-γ(IFN-γ)生成水平等指标的影响,观察肝组织形态学变化。【结果】D-半乳糖诱导的衰老小鼠模型中,OHT和OZT均能控制衰老小鼠体质量(P<0.05),降低血清中的SOD、CAT、GSH-Px、ALT和AST的活性(P<0.05,P<0.01),能显著提高IL-2和IFN-γ生成(P<0.05,P<0.01),降低MDA含量(P<0.05,P<0.01),肝脏病理变化得到改善,OZT干预效果显著优于OHT。【结论】OHT和OZT对D-半乳糖致亚急性衰老动物模型均有很好的干预效果,能有效提高小鼠机体抗氧化能力,延缓衰老的作用。 展开更多
关键词 羟基酪醇 玉米黄质色素 橄榄油 衰老小鼠 抗氧化 抗衰老
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玉米黄质对魔芋葡甘聚糖凝胶性能及热稳定性评价
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作者 任鸿飞 严竟 +2 位作者 彭曼曼 刘雄 张甫生 《食品与发酵工业》 CAS CSCD 北大核心 2024年第4期218-224,共7页
为探究魔芋多糖体系与色素小分子互作的方式并解决魔芋凝胶赋色的问题,该研究以魔芋葡甘聚糖(konjac glucomannan,KGM)凝胶为研究对象,使用玉米黄质(zeaxanthin,ZEA)对KGM凝胶赋色,以不添加色素的KGM凝胶作为对照组,对比分析对照组和赋... 为探究魔芋多糖体系与色素小分子互作的方式并解决魔芋凝胶赋色的问题,该研究以魔芋葡甘聚糖(konjac glucomannan,KGM)凝胶为研究对象,使用玉米黄质(zeaxanthin,ZEA)对KGM凝胶赋色,以不添加色素的KGM凝胶作为对照组,对比分析对照组和赋色后KGM/ZEA复合有色凝胶的凝胶性能和微观结构。结果表明,KGM/ZEA-2复合有色凝胶的色素保留率、L^(*)值、a^(*)值和b^(*)值较对照组分别显著升高了73.82%、9.78%、4.34%、5.63%,较KGM/ZEA-1分别显著升高了16.33%、2.55%、1.09%、2.14%;其持水性、凝胶强度、硬度和咀嚼性较对照组分别显著升高了2.18%、91.01%、54.09%、50.70%,较KGM/ZEA-1显著分别升高了0.88%、75.40%、45.48%、42.23%。微观结构表明,KGM/ZEA-2复合有色凝胶在疏水相互作用的驱动下形成了更加致密的网络结构,可截留更多的色素小分子,同时提高了其结晶度和热稳定性。因此,玉米黄质能够稳定地结合在魔芋凝胶体系内,作为着色剂应用于多彩凝胶素食的开发。 展开更多
关键词 魔芋葡甘聚糖 玉米黄质 复合有色凝胶 凝胶性能 热稳定性 微观结构
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代谢工程改造酿酒酵母合成玉米黄质
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作者 赵崇屹 周景文 徐沙 《食品与发酵工业》 CAS CSCD 北大核心 2024年第5期1-6,共6页
玉米黄质是一种类胡萝卜素,在很多领域都有一定的应用。该研究在前期构建的脂质体积累酿酒酵母的基础上,表达玉米黄质合成酶,初步得到一株能够积累玉米黄质的酿酒酵母工程菌。进一步优化补料碳源、补料时间和补料方式,最终在5 L的发酵罐... 玉米黄质是一种类胡萝卜素,在很多领域都有一定的应用。该研究在前期构建的脂质体积累酿酒酵母的基础上,表达玉米黄质合成酶,初步得到一株能够积累玉米黄质的酿酒酵母工程菌。进一步优化补料碳源、补料时间和补料方式,最终在5 L的发酵罐从48 h开始以指数流加的方式流加葡萄糖,得到玉米黄质的产量为47.7 mg/L。综上,该研究构建了一株高产玉米黄质重组菌并对其进行了发酵验证,研究结果为后续进行发酵优化提供了基础以及改进的方向。 展开更多
关键词 玉米黄质 指数流加 酿酒酵母 脂质载体 补加葡萄糖
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玉米黄质对低温弱光下辣椒光合能力和叶黄素循环的影响
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作者 蒲凯国 丁东霞 +4 位作者 李能慧 张淼 王甜甜 颉建明 李静 《干旱地区农业研究》 CSCD 北大核心 2024年第2期177-187,共11页
为进一步探究玉米黄质对辣椒低温弱光耐受性的影响,以低温敏感性辣椒‘航椒2号’为试验材料,通过叶面喷施玉米黄质,研究其对低温弱光(15℃/5℃,100μmol·m^(-2)·s-1)胁迫下辣椒光合作用关键酶活性、叶绿素荧光参数、内源激素... 为进一步探究玉米黄质对辣椒低温弱光耐受性的影响,以低温敏感性辣椒‘航椒2号’为试验材料,通过叶面喷施玉米黄质,研究其对低温弱光(15℃/5℃,100μmol·m^(-2)·s-1)胁迫下辣椒光合作用关键酶活性、叶绿素荧光参数、内源激素含量、叶黄素循环及相关基因表达量的影响,探究玉米黄质缓解辣椒幼苗低温弱光胁迫的生理机制。结果表明:喷施玉米黄质显著提高辣椒幼苗低温弱光下的光合能力,PSⅡ最大光化学量子产量(F_(v)/F_(m))、实际光化学量子产量Y(Ⅱ)分别显著提高5.90%和54.33%,处理后168 h茉莉酸(JA)和生长素(IAA)含量分别显著增加16.70%和62.30%;辣椒幼苗花药黄质、紫黄质、叶黄素和玉米黄质含量分别增加了1.06、0.48、0.19倍和1.57倍,同时在胁迫处理24、48、168 h时CaZEP基因表达量分别显著增加了3.44、1.64倍和5.50倍,而CaVDE基因表达量与对照相比未达到显著性差异。综上所述,喷施玉米黄质可能通过增强低温弱光下辣椒幼苗光合能力、调控激素代谢以及促进辣椒幼苗启动叶黄素循环来提高辣椒幼苗对低温弱光胁迫的耐受性。 展开更多
关键词 辣椒 外源玉米黄质 低温弱光胁迫 光合能力 叶黄素循环
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膳食叶黄素和玉米黄质与老年人低认知功能的剂量-反应关系
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作者 高蓉 栗卓倩 +4 位作者 王敏 高变芳 徐玥 韩彦青 余红梅 《山西医科大学学报》 CAS 2024年第2期233-238,共6页
目的 使用限制性立方样条回归模型探讨膳食叶黄素和玉米黄质摄入量与老年人低认知功能的剂量-反应关系。方法 基于美国国家健康与营养调查(National Health and Nutrition Examination Survey, NHANES)数据库,选取年龄≥60岁且具有完整... 目的 使用限制性立方样条回归模型探讨膳食叶黄素和玉米黄质摄入量与老年人低认知功能的剂量-反应关系。方法 基于美国国家健康与营养调查(National Health and Nutrition Examination Survey, NHANES)数据库,选取年龄≥60岁且具有完整认知功能测试的老年人群。应用Logistic回归模型和限制性立方样条回归模型评估膳食叶黄素和玉米黄质摄入量与老年人低认知功能的关联和剂量-反应关系。结果 共纳入2 695名研究对象,其中认知功能正常组1 629例,低认知功能组1 066例。与认知功能正常组比较,低认知功能组年龄在70~<80岁和≥80岁的人群所占比例明显较高,糖尿病及中风发生比例也明显增高,此外,膳食叶黄素和玉米黄质摄入量以及叶黄素和玉米黄质总摄入量明显降低,差异均有统计学意义(P<0.05)。限制性立方样条回归分析显示,以性别分层,随着叶黄素和玉米黄质总摄入量的持续增加,男性和女性认知功能下降风险均呈近似“L”形的非线性变化(P非线性<0.01),但是在同样“L”形趋势的情况下,叶黄素和玉米黄质总摄入量是男性认知功能下降的易感因素。结论 叶黄素和玉米黄质总摄入量与老年人认知功能下降风险呈近似“L”形的非线性剂量-反应关系。 展开更多
关键词 叶黄素 玉米黄质 认知功能 限制性立方样条回归 老年人
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Enrichment of health-promoting lutein and zeaxanthin in tomato fruit through metabolic engineering
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作者 Yanjun Wu Yong Yuan +5 位作者 Wenqian Jiang Xin Zhang Siyan Ren Hsihua Wang Xiaolan Zhang Yang Zhang 《Synthetic and Systems Biotechnology》 SCIE 2022年第4期1159-1166,共8页
Carotenoids constitute a large group of natural pigments widely distributed in nature.These compounds not only provide fruits and flowers with distinctive colors,but also have significant health benefits for humans.Lu... Carotenoids constitute a large group of natural pigments widely distributed in nature.These compounds not only provide fruits and flowers with distinctive colors,but also have significant health benefits for humans.Lutein and zeaxanthin,both oxygen-containing carotenoids,are considered to play vital roles in promoting ocular development and maintaining eye health.However,humans and mammals cannot synthesize these carotenoid derivatives,which can only be taken from certain fruits or vegetables.Here,by introducing four endogenous synthetic genes,SlLCYE,SlLCYB,SlHYDB,and SlHYDE under fruit-specific promoters,we report the metabolic engineering of lutein/zeaxanthin biosynthesis in tomato fruit.Transgenic lines overexpression of one(SlLCYE),two(SlLCYE and SlLCYB;SlLCYB and SlHYDB),and all these four synthetic genes re-established the lutein/zeaxanthin biosynthetic pathways in the ripe tomato fruit and thus resulted in various types of carotenoid riched lines.Metabolic analyses of these engineered tomato fruits showed the strategy involved expression of SlLCYE tends to produceα-carotene and lutein,as well as a higher content of β-carotene and zeaxanthin was detected in lines overexpressing SlLCYB.In addition,the different combinations of engineered tomatoes with riched carotenoids showed higher antioxidant capacity and were associated with a significantly extended shelf life during postharvest storage.This work provides a successful example of accurate metabolic engineering in tomato fruit,suggesting the potential utility for synthetic biology to improve agronomic traits in crops.These biofortified tomato fruits could be also exploited as new research subjects for studying the health benefits of carotenoid derivatives. 展开更多
关键词 Solanum lycopersicum Tomato fruit Metabolic engineering LUTEIN zeaxanthin Antioxidant capacity
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枸杞提取物中玉米黄质和叶黄素含量的高效液相测定方法
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作者 唐瑗 康瑶 +1 位作者 於洪建 张东星 《天津科技》 2024年第3期21-25,共5页
选用色谱柱Innoval ODS-2(4.6 mm×250 mm,5µm),以0.05%三乙胺-乙腈为流动相,流速为0.8 mL/min,柱温为28℃,在450 nm波长处测定样品。结果显示:玉米黄质在0.207~20.7µg/mL范围内线性关系良好,判定系数r^(2)=1.0000,平均... 选用色谱柱Innoval ODS-2(4.6 mm×250 mm,5µm),以0.05%三乙胺-乙腈为流动相,流速为0.8 mL/min,柱温为28℃,在450 nm波长处测定样品。结果显示:玉米黄质在0.207~20.7µg/mL范围内线性关系良好,判定系数r^(2)=1.0000,平均回收率为113.72%(n=6),RSD为1.50%,检出限为0.027µg/mL;叶黄素在0.216~21.6µg/mL范围内线性关系良好,判定系数r^(2)=1.0000,平均回收率为105.96%(n=6),RSD为3.78%,检出限为0.056µg/mL。该方法快速、简单稳定、结果准确,适用于同时测定枸杞提取物中叶黄素和玉米黄质。 展开更多
关键词 高效液相色谱法 叶黄素 玉米黄质 枸杞提取物 基线分离
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富含羟基酪醇橄榄油和玉米黄质色素橄榄油对高血脂小鼠的影响 被引量:1
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作者 叶文斌 陈文东 +3 位作者 何九军 邵瑾 邸多隆 王昱 《粮食与油脂》 北大核心 2023年第12期74-78,共5页
研究富含羟基酪醇橄榄油和玉米黄质色素橄榄油对高血脂小鼠血糖、血脂的影响。结果表明:富含羟基酪醇橄榄油和玉米黄质色素橄榄油均能有效降低高血脂小鼠的空腹血糖、糖化血红蛋白、总胆固醇、甘油三酯、低密度脂蛋白、总胆红素、总胆... 研究富含羟基酪醇橄榄油和玉米黄质色素橄榄油对高血脂小鼠血糖、血脂的影响。结果表明:富含羟基酪醇橄榄油和玉米黄质色素橄榄油均能有效降低高血脂小鼠的空腹血糖、糖化血红蛋白、总胆固醇、甘油三酯、低密度脂蛋白、总胆红素、总胆汁酸浓度,升高高密度脂蛋白浓度,对丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、γ-氨酰基转移酶和碱性磷酸酶活性具有很好的抑制作用,能够改善小鼠血糖、血脂代谢,控制小鼠体质量,对小鼠无急慢性毒性。且玉米黄质色素橄榄油效果优于富含羟基酪醇橄榄油。 展开更多
关键词 羟基酪醇 玉米黄质色素 橄榄油
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