Edible zein-based films containing lysozyme(LY) and ascorbic acid(AA) were developed in the presence of polyethylene glycol 400(PEG 400), the combined effects of LY and AA on the microstructure, mechanical properties ...Edible zein-based films containing lysozyme(LY) and ascorbic acid(AA) were developed in the presence of polyethylene glycol 400(PEG 400), the combined effects of LY and AA on the microstructure, mechanical properties and release properties of developed zein films were investigated in detail. The results of microstructure characterization indicated that zein-based films became compact and smooth, and LY aggregates were well distributed in the zein matrix because of the simultaneous addition of LY and AA. The results of mechanical tests showed that because of the synergistic effects of LY and AA on zein film, elongation at break of zein-based film could be up to 138%, which was 34.5 times higher than that of zein control film. LY release tests showed that when the concentration of AA was less than 3.1 mg·cm^(-2), the release rate of LY significantly decreased by 33.7%, and the total release increased by 80.6%. While the release profiles of AA showed that the release rate and total release of AA from the films containing LY increased by approximately 68.9% and 61.7% than the films without LY. Good antioxidant and sustained antimicrobial activities were found for the developed zein films.展开更多
In this study,fucoidan(Fuc)extracted from kelp by water(WFu)and acid(AFu)was combined with zein to prepare nanoparticles.Its potential application as a curcumin(Cur)delivery system was also investigated.The results of...In this study,fucoidan(Fuc)extracted from kelp by water(WFu)and acid(AFu)was combined with zein to prepare nanoparticles.Its potential application as a curcumin(Cur)delivery system was also investigated.The results of chemical composition analysis showed that the sulfate content of AFu(26.44%)was significantly higher than that of WFu(19.65%).The molecular weight(Mw)and monosaccharide composition of WFu and AFu were also different.The anti-solvent precipitation method was used to prepare the nanoparticles.The analysis of nanoparticles with different mass ratios showed that the nanoparticles with a mass ratio of Fuc/zein of 1.5:1 had uniform particle distribution,good stability and the best encapsulation effect.Fourier transform infrared spectroscopy(FT-IR),differential scanning calorimetry(DSC),and fluorescence spectroscopy showed that hydrogen bonds and electrostatic interaction were the main forces responsible for the formation of the nanoparticles.Moreover,Fuc enhanced the thermal and storage stability of zein nanoparticles(ZNP).An in vitro digestion experiment using nanoparticles revealed that Fuc-ZNP had strong tolerance to the gastrointestinal tract and could be used for colon-specific Cur delivery.In addition,antioxidant experiments showed that Fuc significantly increased the antioxidant activity of ZNP(P<0.05).In conclusion,Fuc-ZNP have potential as a Cur delivery system.展开更多
基金Supported by the National Natural Science Foundation of China(21476086)Guangdong Natural Science Foundation(2014A030312007)
文摘Edible zein-based films containing lysozyme(LY) and ascorbic acid(AA) were developed in the presence of polyethylene glycol 400(PEG 400), the combined effects of LY and AA on the microstructure, mechanical properties and release properties of developed zein films were investigated in detail. The results of microstructure characterization indicated that zein-based films became compact and smooth, and LY aggregates were well distributed in the zein matrix because of the simultaneous addition of LY and AA. The results of mechanical tests showed that because of the synergistic effects of LY and AA on zein film, elongation at break of zein-based film could be up to 138%, which was 34.5 times higher than that of zein control film. LY release tests showed that when the concentration of AA was less than 3.1 mg·cm^(-2), the release rate of LY significantly decreased by 33.7%, and the total release increased by 80.6%. While the release profiles of AA showed that the release rate and total release of AA from the films containing LY increased by approximately 68.9% and 61.7% than the films without LY. Good antioxidant and sustained antimicrobial activities were found for the developed zein films.
基金supported by the National Natural Science Foundation of China(No.31701611)the Education Department of Jilin Province of China(No.JJKH20200579KJ)the Young Scholars Climbing Project of Changchun University(No.ZKP202032).
文摘In this study,fucoidan(Fuc)extracted from kelp by water(WFu)and acid(AFu)was combined with zein to prepare nanoparticles.Its potential application as a curcumin(Cur)delivery system was also investigated.The results of chemical composition analysis showed that the sulfate content of AFu(26.44%)was significantly higher than that of WFu(19.65%).The molecular weight(Mw)and monosaccharide composition of WFu and AFu were also different.The anti-solvent precipitation method was used to prepare the nanoparticles.The analysis of nanoparticles with different mass ratios showed that the nanoparticles with a mass ratio of Fuc/zein of 1.5:1 had uniform particle distribution,good stability and the best encapsulation effect.Fourier transform infrared spectroscopy(FT-IR),differential scanning calorimetry(DSC),and fluorescence spectroscopy showed that hydrogen bonds and electrostatic interaction were the main forces responsible for the formation of the nanoparticles.Moreover,Fuc enhanced the thermal and storage stability of zein nanoparticles(ZNP).An in vitro digestion experiment using nanoparticles revealed that Fuc-ZNP had strong tolerance to the gastrointestinal tract and could be used for colon-specific Cur delivery.In addition,antioxidant experiments showed that Fuc significantly increased the antioxidant activity of ZNP(P<0.05).In conclusion,Fuc-ZNP have potential as a Cur delivery system.
