Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(...Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD.展开更多
The fables of Pre-Qin scholars occupied an important position in the history of the development of Chinese culture,thoughts,and literature,hence had profound and extensive influence on later generations.As a kind of c...The fables of Pre-Qin scholars occupied an important position in the history of the development of Chinese culture,thoughts,and literature,hence had profound and extensive influence on later generations.As a kind of carrier of thoughts,the fables of the Pre-Qin scholars were not immutable and frozen,for they have been influenced by various aspects in the process of transmitting and recognizing.This article attempts to study the evolution of the function,expression,and connotation of the fables of the Pre-Qin scholars.展开更多
Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescrip...Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P＞0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P＜0. 01) and TM decreased significantly (P＜0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P＜0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P＜0. 01), P1, TM have also significant(P＜0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P＜0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.展开更多
Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate chan...Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate change and anthropogenic activities. High time-resolution biomarker records of two 210Pb-dated sediment cores（#34： 28.5°N, 122.272°E; CJ12-1269： 28.861 9°N, 122.515 3°E） from the Min-Zhe coastal mud area were compared to reveal changes of phytoplankton productivity and community structure over the past 100 years. Phytoplankton productivity started to increase gradually from the 1970 s and increased rapidly after the late 1990 s at Site #34; and it started to increase gradually from the middle 1960 s and increased rapidly after the late 1980 s at Site CJ12-1269. Productivity of Core CJ12-1269 was higher than that of Core #34. Phytoplankton community structure variations displayed opposite patterns in the two cores. The decreasing D/B（dinosterol/brassicasterol） ratio of Core #34 since the 1960 s revealed increased diatom contribution to total productivity. In contrast, the increasing D/B ratio of Core CJ12-1269 since the 1950 s indicated increased dinoflagellate contribution to total productivity. Both the productivity increase and the increased dinoflagellate contribution in Core CJ12-1269 since the 1950–1960s were mainly caused by anthropogenic activities, as the location was closer to the Changjiang River Estuary with higher nutrient concentration and decreasing Si/N ratios. However, increased diatom contribution in Core #34 is proposed to be caused by increased coastal upwelling, with higher nutrient concentration and higher Si/N ratios.展开更多
基金supported by Ningbo Natural Science Foundation(Grant No.:2022J229)Project of Ningbo Leading Medical&Health Discipline(Project No.:2022-S04)+1 种基金Opened-end Fund of Key Laboratory(Grant No.:KFJJ-202101)Graduate Student Scientific Research and Innovation Fund of Ningbo University(Grant No.:IF2022193)。
文摘Nonalcoholic fatty liver disease(NAFLD)has developed into the most common chronic liver disease and can lead to liver cancer.Our laboratory previously developed a novel prescription for NAFLD,“Eight Zhes Decoction”(EZD),which has shown good curative effects in clinical practice.However,the pharmacodynamic material basis and mechanism have not yet been revealed.A strategy integrating lipidomics,network pharmacology and pharmacokinetics was used to reveal the active components and mechanisms of EZD against NAFLD.The histopathological results showed that EZD attenuated the degrees of collagen deposition and steatosis in the livers of nonalcoholic steatofibrosis model mice.Furthermore,glycerophospholipid metabolism,arachidonic acid metabolism,glycerolipid metabolism and linoleic acid metabolism with phospholipase A2 group IVA(PLA2G4A)and cytochrome P450 as the core targets and 12,13-cis-epoxyoctadecenoic acid,12(S)-hydroxyeicosatetraenoic acid,leukotriene B4,prostaglandin E2,phosphatidylcholines(PCs)and triacylglycerols(TGs)as the main lipids were found to be involved in the treatment of NAFLD by EZD.Importantly,naringenin,artemetin,canadine,and bicuculline were identified as the active ingredients of EZD against NAFLD;in particular,naringenin reduces PC consumption by inhibiting the expression of PLA2G4A and thus promotes sufficient synthesis of very-low-density lipoprotein to transport excess TGs in the liver.This research provides valuable data and theoretical support for the application of EZD against NAFLD.
基金北京市大学生创新训练项目-先秦子部“寓言”文本生成与传播路径研究,Serial Number S202210015036北京印刷学院校级教改创新重点项目-国家级一流专业--编辑出版学专业建设研究,Serial Number22150223075.
文摘The fables of Pre-Qin scholars occupied an important position in the history of the development of Chinese culture,thoughts,and literature,hence had profound and extensive influence on later generations.As a kind of carrier of thoughts,the fables of the Pre-Qin scholars were not immutable and frozen,for they have been influenced by various aspects in the process of transmitting and recognizing.This article attempts to study the evolution of the function,expression,and connotation of the fables of the Pre-Qin scholars.
文摘Objective To develop a plasma pharmacological method evaluates the effect of Da Huang Zhe Chong capsule on platelet aggregation and its mechanism, which is a representative Traditional Chinese Medicine Patent Prescription Promoting blood circulation by removing blood stasis. Methods Platelets specimens from healthy volunteers made serum and plasma with medicine, while platelet PRP were separated, which were divided into 8groups,i.e. auto-serum, allo-serum, serum with Da Huang Zhe Chong capsule , serum with aspirin, auto-plasma, plasma with Da Huang Zhe Chong capsule, plasma with aspirin, every group added to serum and plasma to hatch. After ADP and adrenalin were added into the specimens and hatched, the effects of specimens on platelet aggregation were observed. Results After ADP adrenalin were added, all the serum groups did not present platelet aggregation,while all the plasma group presented platelet aggregation. P1, P5, Pmax, t and TM have no significant difference (P＞0. 05) between auto-plasma group and allo-plasma group induced by ADP and adrenalin. P1, P5, t and Pmax have significant differences (P＜0. 01) and TM decreased significantly (P＜0. 05) comparing plasma group with Da Huang Zhe Chong capsule and plasma group of aspirin to allo-plasma group. P1, t and Pmax have significant difference (P＜0.05), and P5 and TM are simulate comparing plasma group with Da Huang Zhe Chong capsule to plasma group of aspirin. P1, P5, t and Pmax have significant differences (P＜0. 01), P1, TM have also significant(P＜0. 05), comparing plasma group of Da Huang Zhe Chong capsule with plasma of aspirin to allo-plasma group induced by adrenalin. P1 ,P5 and Pmax have significant differences (P＜0.05), and t and Pmax are simulate comparing plasma group with Da Huang Zhe Chong capsule with plasma group of aspirin. Conclusion The serum pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study is inappropriate to study platelet aggregation in vitro. The plasma pharmacological study could reflect the pharmacological effect produced in vivo. Da Huang Zhe Chong capsule has better anti artery thrombosis effect than aspirin, and it is an ideal medicine for anti artery thrombosis.
基金The National Basic Research Program of China(973 Program)under contract No.2010CB428901the National Natural Science Foundation of China under contract Nos 41020164005,40976042 and 41076036
文摘Phytoplankton productivity and community structure in marginal seas have been altered significantly during the past three decades, but it is still a challenge to distinguish the forcing mechanisms between climate change and anthropogenic activities. High time-resolution biomarker records of two 210Pb-dated sediment cores（#34： 28.5°N, 122.272°E; CJ12-1269： 28.861 9°N, 122.515 3°E） from the Min-Zhe coastal mud area were compared to reveal changes of phytoplankton productivity and community structure over the past 100 years. Phytoplankton productivity started to increase gradually from the 1970 s and increased rapidly after the late 1990 s at Site #34; and it started to increase gradually from the middle 1960 s and increased rapidly after the late 1980 s at Site CJ12-1269. Productivity of Core CJ12-1269 was higher than that of Core #34. Phytoplankton community structure variations displayed opposite patterns in the two cores. The decreasing D/B（dinosterol/brassicasterol） ratio of Core #34 since the 1960 s revealed increased diatom contribution to total productivity. In contrast, the increasing D/B ratio of Core CJ12-1269 since the 1950 s indicated increased dinoflagellate contribution to total productivity. Both the productivity increase and the increased dinoflagellate contribution in Core CJ12-1269 since the 1950–1960s were mainly caused by anthropogenic activities, as the location was closer to the Changjiang River Estuary with higher nutrient concentration and decreasing Si/N ratios. However, increased diatom contribution in Core #34 is proposed to be caused by increased coastal upwelling, with higher nutrient concentration and higher Si/N ratios.
