Objective Observation of the effects of lower concentrations of zinc (20 ～ 400 μmol /L), which is nearby the physiological concentration, on etoposide induced HL-60 cells apoptosis. Methods Us-ing the flow cytometry...Objective Observation of the effects of lower concentrations of zinc (20 ～ 400 μmol /L), which is nearby the physiological concentration, on etoposide induced HL-60 cells apoptosis. Methods Us-ing the flow cytometry, DNA extraction, electrophoresis, and fluoresence microscopic observation. Results We demonstrated that The low concentrations of zinc also affect cells apoptosis. If zinc was added at early time, even 200μmol/L could inhibit V16 induced HL-60 cell apoptosis completely in 4h. But at this concentration, zinc also seems to have cell toxicity, not prolong the time of protection effect. Conclusion Zinc plays mul-tiple roles in cellular functions and in protecting cells from exogenous deleterious factors. Zinc plays a complex, dose- and time-dependent role in apoptosis.展开更多
文摘Objective Observation of the effects of lower concentrations of zinc (20 ～ 400 μmol /L), which is nearby the physiological concentration, on etoposide induced HL-60 cells apoptosis. Methods Us-ing the flow cytometry, DNA extraction, electrophoresis, and fluoresence microscopic observation. Results We demonstrated that The low concentrations of zinc also affect cells apoptosis. If zinc was added at early time, even 200μmol/L could inhibit V16 induced HL-60 cell apoptosis completely in 4h. But at this concentration, zinc also seems to have cell toxicity, not prolong the time of protection effect. Conclusion Zinc plays mul-tiple roles in cellular functions and in protecting cells from exogenous deleterious factors. Zinc plays a complex, dose- and time-dependent role in apoptosis.
