Objective To determine whether supplementation of zinc and vitamin A may improve the function of T cells in human PBMC. Methods T cells were separated and cultured in vitro, supplemented with either Zn or vitamin A al...Objective To determine whether supplementation of zinc and vitamin A may improve the function of T cells in human PBMC. Methods T cells were separated and cultured in vitro, supplemented with either Zn or vitamin A alone, or both Zn and vitamin A （10^-6 mol/L, 10^-5 mol/L, 10^-4 mol/L）. After harvesting, cell proliferation, cell cycle, apoptosis, expression or function of cell-surface molecules, such as CD^3＋, CD^4＋, and CD^8＋ were detected. Results Higher proliferation level and lower apoptosis level were observed in cells supplemented with both Zn and vitaminA, showing the strongest effect （P〈0.05）. Zn-supplement increased the CD^4＋/CD^3＋ cell percentage, and simultaneously decreased the CD^8＋/CD^3＋ cell population. VA-supplement showed the opposite effect in comparison with Zn-supplement. Conclusion T-cell function can be improved depending on the zinc and/or vitamin A supplemented.展开更多
Objective: TO assess the effects of zinc supplementation to Chinese rural pregnant with cereal-based diet and their pregnancy outcome. Methods A total of 156 women were given randomly in double blind 5mg/d (group A, G...Objective: TO assess the effects of zinc supplementation to Chinese rural pregnant with cereal-based diet and their pregnancy outcome. Methods A total of 156 women were given randomly in double blind 5mg/d (group A, GPA, n=37),10mg/d (group B, GPB, n=40), 30mg (group C, GpC, n = 39) Of zinc supplement or placebo (group D, GpD, n = 40 ) starting from, the first visit at the prenatal clinic (average 9-week gestation ) till the delivery. Results Mean dietary zinc intake of these women was about 10mg/d, and more than 65% of dietary zinc were taken from cereals, grains and vegetables. Serum zinc concentrations at 25-and 32-gestation in GpC and in the umbilical cord blood of their babies at birth significantly increased. Infants in GpC had a significantly heavier birth weight (283. 079, P = 0. 016 ) and larger head circumference(0. 63cm, P = 0. 035) than infants in GpD. The incidence of low birth weight, preterm and intrauterine growth retardation infants was significantly reduced in GpC. As compared to GpD (38. 34 ± 1. 34 weeks), the duration of gestation in GpD(39. 12± 1. 11 weeks) was prolonged (P= 0. 032). Serum zinc concentration at 25-weeks gestation was negatively correlated with duration of gestation (P = 0. 009 ). Apgar score of the infants in GpC was significantly higher than that in GpD, and serum zinc concentration at 32-weeks gestation was positively associated with infant Apgar score (P = 0. 015 ). Conclusion Zinc supplementation in 30mg/d to Chinese rural women during pregnancy could significantly improve their pregnancy outcome. Both 5mg/d and 10mg/d of zinc supplementation had no detected benefits to them.展开更多
The underlying pathophysiology of liver dysfunction in urea cycle disorders(UCDs) is still largely elusive. There is some evidence that the accumulation of urea cycle(UC) intermediates are toxic for hepatocyte mitocho...The underlying pathophysiology of liver dysfunction in urea cycle disorders(UCDs) is still largely elusive. There is some evidence that the accumulation of urea cycle(UC) intermediates are toxic for hepatocyte mitochondria. It is possible that liver injury is directly caused by the toxicity of ammonia. The rarity of UCDs, the lack of checking of iron level in these patients, superficial knowledge of UC and an underestimation of the metabolic role of fumaric acid, are the main reasons that are responsible for the incomprehension of the mechanism of liver injury in patients suffering from UCDs. Owing to our routine clinical practice to screen for iron overload in severely ill neonates, with the focus on the newborns suffering from acute liver failure, we report a case of citrullinemia with neonatal liver failure and high blood parameters of iron overload. We hypothesize that the key is in the decreased-deficient fumaric acid production in the course of UC in UCDs that causes several sequentially intertwined metabolic disturbances with final result of liver iron overload. The presented hypothesis could be easily tested by examining the patients suffering from UCDs, for liver iron overload. This could be easily performed in countries with a high population and comprehensive national register for inborn errors of metabolism. Conclusion: Providing the hypothesis is correct, neonatal liver damage in patients having UCD can be prevented by the supplementation of pregnant women with fumaric or succinic acid, prepared in the form of iron supplementation pills. After birth, liverdamage in patients having UCDs can be prevented by supplementation of these patients with zinc fumarate or zinc succinylate, as well.展开更多
A multicenter, open-label, randomized, controlled superiority trial with 18 months of follow-up was conducted to investigate whether oral zinc supplementation could further promote spermatogenesis in males with isolat...A multicenter, open-label, randomized, controlled superiority trial with 18 months of follow-up was conducted to investigate whether oral zinc supplementation could further promote spermatogenesis in males with isolated hypogonadotropic hypogonadism （IHH） receiving sequential purified urinary follicular-stimulating hormone/human chorionic gonadotropin （uFSH/hCG） replacement. Sixty-seven Chinese male IHH patients were recruited from the Departments of Endocrinology in eight tertiary hospitals and randomly allocated into the sequential uFSH/hCG group （Group A, n = 34） or the sequential uFSH plus zinc supplementation group （Group B, n = 33）. In Group A, patients received sequential uFSH （75 U, three times a week every other 3 months） and hCG （2000 U, twice a week） treatments. In Group B, patients received oral zinc supplementation （40 mg day-1） in addition to the sequential uFSH/hCG treatment given to patients in Group A. The primary outcome was the proportion of patients with a sperm concentration 〉1.0 × 106 ml-1 during the 18 months. The comparison of efficacy between Groups A and B was analyzed. Nineteen of 34 （55.9%） patients receiving sequential uFSH/hCG and 20 of 33 （60.6%） patients receiving sequential uFSH/hCG plus zinc supplementation achieved sperm concentrations ≥1.0 × 106 ml-1 by intention to treat analyses. No differences between Group A and Group B were observed as far as the efficacy of inducing spermatogenesis （P = 0.69）. We concluded that the sequential uFSH/hCG plus zinc supplementation regimen had a similar efficacy to the sequential uFSH/hCG treatment alone. The additional improvement of 40 mg day-1 oral zinc supplementation on spermatogenesis and masculinization in male IHH patients is very subtle.展开更多
Zinc deficiency is one of the serious problems of trace element nutrition in the world, which causes a series of pathologic effects. Based on the principles of bioinorganic chemistry, the ternary complexes of Zn-lysiu...Zinc deficiency is one of the serious problems of trace element nutrition in the world, which causes a series of pathologic effects. Based on the principles of bioinorganic chemistry, the ternary complexes of Zn-lysiue-histidine were studied as a substitution of zinc sulfate for zinc supplementation. It has also been proved by animal trail.展开更多
基金This work was supported by Grant [2005]383 from the Scientific Research Foundation for the Returned Overseas Chinese Scholars.
文摘Objective To determine whether supplementation of zinc and vitamin A may improve the function of T cells in human PBMC. Methods T cells were separated and cultured in vitro, supplemented with either Zn or vitamin A alone, or both Zn and vitamin A （10^-6 mol/L, 10^-5 mol/L, 10^-4 mol/L）. After harvesting, cell proliferation, cell cycle, apoptosis, expression or function of cell-surface molecules, such as CD^3＋, CD^4＋, and CD^8＋ were detected. Results Higher proliferation level and lower apoptosis level were observed in cells supplemented with both Zn and vitaminA, showing the strongest effect （P〈0.05）. Zn-supplement increased the CD^4＋/CD^3＋ cell percentage, and simultaneously decreased the CD^8＋/CD^3＋ cell population. VA-supplement showed the opposite effect in comparison with Zn-supplement. Conclusion T-cell function can be improved depending on the zinc and/or vitamin A supplemented.
文摘Objective: TO assess the effects of zinc supplementation to Chinese rural pregnant with cereal-based diet and their pregnancy outcome. Methods A total of 156 women were given randomly in double blind 5mg/d (group A, GPA, n=37),10mg/d (group B, GPB, n=40), 30mg (group C, GpC, n = 39) Of zinc supplement or placebo (group D, GpD, n = 40 ) starting from, the first visit at the prenatal clinic (average 9-week gestation ) till the delivery. Results Mean dietary zinc intake of these women was about 10mg/d, and more than 65% of dietary zinc were taken from cereals, grains and vegetables. Serum zinc concentrations at 25-and 32-gestation in GpC and in the umbilical cord blood of their babies at birth significantly increased. Infants in GpC had a significantly heavier birth weight (283. 079, P = 0. 016 ) and larger head circumference(0. 63cm, P = 0. 035) than infants in GpD. The incidence of low birth weight, preterm and intrauterine growth retardation infants was significantly reduced in GpC. As compared to GpD (38. 34 ± 1. 34 weeks), the duration of gestation in GpD(39. 12± 1. 11 weeks) was prolonged (P= 0. 032). Serum zinc concentration at 25-weeks gestation was negatively correlated with duration of gestation (P = 0. 009 ). Apgar score of the infants in GpC was significantly higher than that in GpD, and serum zinc concentration at 32-weeks gestation was positively associated with infant Apgar score (P = 0. 015 ). Conclusion Zinc supplementation in 30mg/d to Chinese rural women during pregnancy could significantly improve their pregnancy outcome. Both 5mg/d and 10mg/d of zinc supplementation had no detected benefits to them.
文摘The underlying pathophysiology of liver dysfunction in urea cycle disorders(UCDs) is still largely elusive. There is some evidence that the accumulation of urea cycle(UC) intermediates are toxic for hepatocyte mitochondria. It is possible that liver injury is directly caused by the toxicity of ammonia. The rarity of UCDs, the lack of checking of iron level in these patients, superficial knowledge of UC and an underestimation of the metabolic role of fumaric acid, are the main reasons that are responsible for the incomprehension of the mechanism of liver injury in patients suffering from UCDs. Owing to our routine clinical practice to screen for iron overload in severely ill neonates, with the focus on the newborns suffering from acute liver failure, we report a case of citrullinemia with neonatal liver failure and high blood parameters of iron overload. We hypothesize that the key is in the decreased-deficient fumaric acid production in the course of UC in UCDs that causes several sequentially intertwined metabolic disturbances with final result of liver iron overload. The presented hypothesis could be easily tested by examining the patients suffering from UCDs, for liver iron overload. This could be easily performed in countries with a high population and comprehensive national register for inborn errors of metabolism. Conclusion: Providing the hypothesis is correct, neonatal liver damage in patients having UCD can be prevented by the supplementation of pregnant women with fumaric or succinic acid, prepared in the form of iron supplementation pills. After birth, liverdamage in patients having UCDs can be prevented by supplementation of these patients with zinc fumarate or zinc succinylate, as well.
文摘A multicenter, open-label, randomized, controlled superiority trial with 18 months of follow-up was conducted to investigate whether oral zinc supplementation could further promote spermatogenesis in males with isolated hypogonadotropic hypogonadism （IHH） receiving sequential purified urinary follicular-stimulating hormone/human chorionic gonadotropin （uFSH/hCG） replacement. Sixty-seven Chinese male IHH patients were recruited from the Departments of Endocrinology in eight tertiary hospitals and randomly allocated into the sequential uFSH/hCG group （Group A, n = 34） or the sequential uFSH plus zinc supplementation group （Group B, n = 33）. In Group A, patients received sequential uFSH （75 U, three times a week every other 3 months） and hCG （2000 U, twice a week） treatments. In Group B, patients received oral zinc supplementation （40 mg day-1） in addition to the sequential uFSH/hCG treatment given to patients in Group A. The primary outcome was the proportion of patients with a sperm concentration 〉1.0 × 106 ml-1 during the 18 months. The comparison of efficacy between Groups A and B was analyzed. Nineteen of 34 （55.9%） patients receiving sequential uFSH/hCG and 20 of 33 （60.6%） patients receiving sequential uFSH/hCG plus zinc supplementation achieved sperm concentrations ≥1.0 × 106 ml-1 by intention to treat analyses. No differences between Group A and Group B were observed as far as the efficacy of inducing spermatogenesis （P = 0.69）. We concluded that the sequential uFSH/hCG plus zinc supplementation regimen had a similar efficacy to the sequential uFSH/hCG treatment alone. The additional improvement of 40 mg day-1 oral zinc supplementation on spermatogenesis and masculinization in male IHH patients is very subtle.
基金Project supported by the National Natural Science Foundation of China.
文摘Zinc deficiency is one of the serious problems of trace element nutrition in the world, which causes a series of pathologic effects. Based on the principles of bioinorganic chemistry, the ternary complexes of Zn-lysiue-histidine were studied as a substitution of zinc sulfate for zinc supplementation. It has also been proved by animal trail.
