In order to find out a new pathway for utilizing heterosis of Zinnia elegans and accelerate breeding process, the mechanism of anther development of a male sterile line was explored. Backcross, sibmating, selling of f...In order to find out a new pathway for utilizing heterosis of Zinnia elegans and accelerate breeding process, the mechanism of anther development of a male sterile line was explored. Backcross, sibmating, selling of fertile plants and testcross with inbred lines were analyzed and identified in the field, and cytology was observed. Recessive nucleus male sterile line AH209AB capable of being a maintainer was obtained by successive backcrosses with male sterile plants and fertile F1 plants as male parents. Cytological and anatomical studies indicated that: (1) The wall of normal anther was constituted of four layers of cells such as epidermis, powder chamber wall, middle level and tapetum cells. The process in meiosis of pollen mother cell in Zinnia elegans was normal and cytoplasm divided simultanously. Mature pollen grain was tricellular type. (2) The petal of male sterile plant degraded as a thread-like structure, the stamens were villiform in appearance and no pollens were formed. The result showed that the anther of male sterile plant no longer proceed to differentiate spore mother cell and the pollen sac after the formation of the tissue of sporogenous cells, there was no evident boundary between tapetum cell, middle lamella and inner wall of PMC, tapetal cells did not develop from the very beginning. So the abortion type was completely structural male sterility. The male sterile line belongs to non-sporange male sterile type and is of great use in F1 seeds production.展开更多
In order to isolate genes related to anther development and understand molecular basis of male sterility,cDNA library of Zinnia elegans was constructed using suppression subtractive hybridization(SSH) approach.672 d...In order to isolate genes related to anther development and understand molecular basis of male sterility,cDNA library of Zinnia elegans was constructed using suppression subtractive hybridization(SSH) approach.672 different expressed clones were selected from the fertile disk floret buds.PCR results showed that cDNA inserts were ranged from 100 to 750 bp.303 positive clones screened by dot-blot hybridization were sequenced.273 out of 303 sequenced clones produced readable sequences;these sequences represent 87 non-repetitive sequences.The homology alignment showed that 76 expressed sequence tags(ESTs) had functional annotations in GenBank,the other 11 ESTs without any homology to the known gene.In addition,87 ESTs were divided into 17 groups according to MIPS of Arabidopsis thaliana database.Sequence data from the cDNA library have been deposited with the GenBank under the accession numbers GT067016-GT067085.As an important result in this study,7 genes related to anther development were isolated.Results from semi-quantitative RT-PCR showed 6 genes were expressed only in disk florets of fertile plants compared with that of male sterile plants.These ESTs obtained provide important clues for further isolation and identification of fertility-related genes in Z.elegans.展开更多
In this study, we compared plant height, weight, soil TPH concentration, and soil DHA level after 18 weeks of </span><i><span style="font-family:Verdana;">Zennia</span></i><s...In this study, we compared plant height, weight, soil TPH concentration, and soil DHA level after 18 weeks of </span><i><span style="font-family:Verdana;">Zennia</span></i><span style="font-family:Verdana;"> <i>hybrida</i> </span><span style="font-family:Verdana;">cultivation with four different concentrations of 5-Aminolevulinic acid (ALA)-based liquid fertilizer: 1500-fold, 5000-fold, and 8000-fold dilutions, along with a non-treatment control of diluted ALA. The plants of ALA-treated were significantly taller than the non-treatment control. The plants of ALA-treated plants were higher in shoot fresh weight, shoot dry weight, and root dry weight than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold dilution was significantly highest in shoot fresh weight, shoot dry weight, and root dry weight. ALA-treated plants were lower in the soil Total Petroleum </span></span><span style="font-family:Verdana;">Hydrocarbon </span><span style="font-family:""><span style="font-family:Verdana;">(TPH) concentration than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold was significantly lowest in the TPH concentration. In addition, ALA-treated plants were </span><span style="font-family:Verdana;">higher in the soil dehydrogenase activity (DHA) than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold was signifi</span><span style="font-family:Verdana;">cantly highest in the TPH concentration. This study indicated that ALA-applied zinnia-grown oil-contaminated soil is more effective than not. The remediation in oil-contaminated soil with ALA-based liquid fertilizer is more effective than the non-treatment control</span></span><span style="font-family:Verdana;">;</span><span style="font-family:Verdana;">furthermore, ALA application with 5000-fold dilution was most suitable in oil-contaminated soil among other plots.展开更多
[Objectives] The research aimed to study the effect of sludge extract on the growth of Zinnia elegans, and evaluate its possibility of application. [Methods] Z. elegans was taken as research object, and the effect of ...[Objectives] The research aimed to study the effect of sludge extract on the growth of Zinnia elegans, and evaluate its possibility of application. [Methods] Z. elegans was taken as research object, and the effect of sludge extract on the growth indicators of Z. elegans was studied. [Results] The sludge extract can obviously promote the growth of Z. elegans, and the growth indicators were significantly improved when compared with the control group. The application effect was close to that of foliar fertilizer purchased in the market. In each treatment group, the low concentration of sludge extract did not significantly promote the growth of Z. elegans, while too high concentration could inhibit its growth, and the optimal dilution ratio was 600 times. [Conclusions] Further preparation of amino acid foliar fertilizer from sludge extract and its application in planting garden plants can realize the resource utilization of sludge and has a good development and application prospect.展开更多
Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid (GA3) exerts an opposite effect to that exerted by l...Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid (GA3) exerts an opposite effect to that exerted by light on hypocotyl photomorphogenesis because GAz promotes an etiolated-like growth with an inhibition of radial (secondary) growth. For this reason, the effect of GA3 on the basic peroxidase isoenzyme from Z. elegans (ZePrx), an enzyme involved in hypocotyl lignin biosynthesis, was studied. The results showed that GA3 reduces ZePrx activity, similarly to the way in which it reduces seedling secondary growth. This hormonal response is supported by the analysis of the ZePrx promoter, which contains four types of GA3-responsive cis.elements: the W Box/O2S; the Pyr Box; the GARE; and the Amy Box. Taken together, these results suggest that ZePrx is directly regulated by GA3, with this effect matching the inhibitory effect of GA on the hypocotyl secondary growth.展开更多
基金supported by the National Natural Science Foundation of China(30771518)948 Program under the Ministry of Agriculture,China(2003-Z36).
文摘In order to find out a new pathway for utilizing heterosis of Zinnia elegans and accelerate breeding process, the mechanism of anther development of a male sterile line was explored. Backcross, sibmating, selling of fertile plants and testcross with inbred lines were analyzed and identified in the field, and cytology was observed. Recessive nucleus male sterile line AH209AB capable of being a maintainer was obtained by successive backcrosses with male sterile plants and fertile F1 plants as male parents. Cytological and anatomical studies indicated that: (1) The wall of normal anther was constituted of four layers of cells such as epidermis, powder chamber wall, middle level and tapetum cells. The process in meiosis of pollen mother cell in Zinnia elegans was normal and cytoplasm divided simultanously. Mature pollen grain was tricellular type. (2) The petal of male sterile plant degraded as a thread-like structure, the stamens were villiform in appearance and no pollens were formed. The result showed that the anther of male sterile plant no longer proceed to differentiate spore mother cell and the pollen sac after the formation of the tissue of sporogenous cells, there was no evident boundary between tapetum cell, middle lamella and inner wall of PMC, tapetal cells did not develop from the very beginning. So the abortion type was completely structural male sterility. The male sterile line belongs to non-sporange male sterile type and is of great use in F1 seeds production.
基金funded by the National Natural Science Foundation of China(30771518)
文摘In order to isolate genes related to anther development and understand molecular basis of male sterility,cDNA library of Zinnia elegans was constructed using suppression subtractive hybridization(SSH) approach.672 different expressed clones were selected from the fertile disk floret buds.PCR results showed that cDNA inserts were ranged from 100 to 750 bp.303 positive clones screened by dot-blot hybridization were sequenced.273 out of 303 sequenced clones produced readable sequences;these sequences represent 87 non-repetitive sequences.The homology alignment showed that 76 expressed sequence tags(ESTs) had functional annotations in GenBank,the other 11 ESTs without any homology to the known gene.In addition,87 ESTs were divided into 17 groups according to MIPS of Arabidopsis thaliana database.Sequence data from the cDNA library have been deposited with the GenBank under the accession numbers GT067016-GT067085.As an important result in this study,7 genes related to anther development were isolated.Results from semi-quantitative RT-PCR showed 6 genes were expressed only in disk florets of fertile plants compared with that of male sterile plants.These ESTs obtained provide important clues for further isolation and identification of fertility-related genes in Z.elegans.
文摘In this study, we compared plant height, weight, soil TPH concentration, and soil DHA level after 18 weeks of </span><i><span style="font-family:Verdana;">Zennia</span></i><span style="font-family:Verdana;"> <i>hybrida</i> </span><span style="font-family:Verdana;">cultivation with four different concentrations of 5-Aminolevulinic acid (ALA)-based liquid fertilizer: 1500-fold, 5000-fold, and 8000-fold dilutions, along with a non-treatment control of diluted ALA. The plants of ALA-treated were significantly taller than the non-treatment control. The plants of ALA-treated plants were higher in shoot fresh weight, shoot dry weight, and root dry weight than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold dilution was significantly highest in shoot fresh weight, shoot dry weight, and root dry weight. ALA-treated plants were lower in the soil Total Petroleum </span></span><span style="font-family:Verdana;">Hydrocarbon </span><span style="font-family:""><span style="font-family:Verdana;">(TPH) concentration than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold was significantly lowest in the TPH concentration. In addition, ALA-treated plants were </span><span style="font-family:Verdana;">higher in the soil dehydrogenase activity (DHA) than the non-treatment control. The plot of ALA-based liquid fertilizer with the 5000-fold was signifi</span><span style="font-family:Verdana;">cantly highest in the TPH concentration. This study indicated that ALA-applied zinnia-grown oil-contaminated soil is more effective than not. The remediation in oil-contaminated soil with ALA-based liquid fertilizer is more effective than the non-treatment control</span></span><span style="font-family:Verdana;">;</span><span style="font-family:Verdana;">furthermore, ALA application with 5000-fold dilution was most suitable in oil-contaminated soil among other plots.
基金Supported by Science and Technology Top Talent Support Project of Guizhou Provincial Department of Education (QIANJIAOHE KY ZI[2018]044)Innovation and Entrepreneurship Training Program for College Students(201510672007,201710672011)。
文摘[Objectives] The research aimed to study the effect of sludge extract on the growth of Zinnia elegans, and evaluate its possibility of application. [Methods] Z. elegans was taken as research object, and the effect of sludge extract on the growth indicators of Z. elegans was studied. [Results] The sludge extract can obviously promote the growth of Z. elegans, and the growth indicators were significantly improved when compared with the control group. The application effect was close to that of foliar fertilizer purchased in the market. In each treatment group, the low concentration of sludge extract did not significantly promote the growth of Z. elegans, while too high concentration could inhibit its growth, and the optimal dilution ratio was 600 times. [Conclusions] Further preparation of amino acid foliar fertilizer from sludge extract and its application in planting garden plants can realize the resource utilization of sludge and has a good development and application prospect.
基金supported by grants from the MEC (BFU2006-11577/BFI)-FEDERCARM (Programa de Generación de Conocimiento Científico de Excelencia de la Fundación Sneca,Agencia de Ciencia y Tecnología de la Región de Murcia en el Marco del Ⅱ PCTRM 2007–2010,project 08610/PI/08)
文摘Hypocotyl formation during the epigeal germination of seedlings is under strict hormonal regulation. In a 3 d old Zinnia elegans seedling system, gibberellic acid (GA3) exerts an opposite effect to that exerted by light on hypocotyl photomorphogenesis because GAz promotes an etiolated-like growth with an inhibition of radial (secondary) growth. For this reason, the effect of GA3 on the basic peroxidase isoenzyme from Z. elegans (ZePrx), an enzyme involved in hypocotyl lignin biosynthesis, was studied. The results showed that GA3 reduces ZePrx activity, similarly to the way in which it reduces seedling secondary growth. This hormonal response is supported by the analysis of the ZePrx promoter, which contains four types of GA3-responsive cis.elements: the W Box/O2S; the Pyr Box; the GARE; and the Amy Box. Taken together, these results suggest that ZePrx is directly regulated by GA3, with this effect matching the inhibitory effect of GA on the hypocotyl secondary growth.