期刊文献+
共找到142篇文章
< 1 2 8 >
每页显示 20 50 100
A new anthraquinone glycoside from seeds of Cassia obtusifolia 被引量:8
1
作者 Li Ying Tang Zhu Ju Wang Mei Hong Fu Yan He Hong Wei Wu Lu Qi Huang 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第9期1083-1085,共3页
A new anthraquinone glycoside 1, along with a known anthraquinone glycoside aurantio-obmsin-6-O-β-D-glucopyranoside 2, were isolated from the seeds of Cassia obtusifolia. On the basis of spectral and chemical evidenc... A new anthraquinone glycoside 1, along with a known anthraquinone glycoside aurantio-obmsin-6-O-β-D-glucopyranoside 2, were isolated from the seeds of Cassia obtusifolia. On the basis of spectral and chemical evidences, the structure of 1 was established as 1-demethylaurantio-obtusin-2-O-β-D-glucopyranoside. Moreover, the 13C NMR of 2 was assigned totally and correctly for the first time based on the two-dimensional NMR. 展开更多
关键词 ~assia obtusifolia Anthraquinone glycoside 1-Demethylaurantio-obtusin-2-O-β-D-glucopyranoside
下载PDF
豆瓣绿(Peperomia obtusifolia)的组织培养与快速繁殖研究 被引量:8
2
作者 王涛 彭立新 《天津农学院学报》 CAS 2011年第2期1-5,共5页
介绍了以豆瓣绿带芽茎段、叶片、花序为外植体的组织培养和快速繁殖研究。结果表明:可用于豆瓣绿组织培养的最适外植体为带芽茎段。诱导芽生长的最适培养基为MS+6-BA1.00 mg.L-1+NAA 1.00 mg.L-1+VC60 mg.L-1,诱导率为95%。诱导芽增殖... 介绍了以豆瓣绿带芽茎段、叶片、花序为外植体的组织培养和快速繁殖研究。结果表明:可用于豆瓣绿组织培养的最适外植体为带芽茎段。诱导芽生长的最适培养基为MS+6-BA1.00 mg.L-1+NAA 1.00 mg.L-1+VC60 mg.L-1,诱导率为95%。诱导芽增殖的最适培养基为MS+6-BA 1.00 mg.L-1+NAA 0.03 mg.L-1+KT2.50 mg.L-1+GA30.05 mg.L-1,增殖倍数为10。生根培养基的最适配方为1/2 MS+NAA 0.25 mg.L-1+蔗糖2%。 展开更多
关键词 豆瓣绿 外植体 组织培养
下载PDF
A New Anthranquinone Glycoside from the Seeds of Cassia obtusifolia 被引量:12
3
作者 ChuHuaLI XiaoYiWEI +2 位作者 XuELI PingWU BaoJiangGUO 《Chinese Chemical Letters》 SCIE CAS CSCD 2004年第12期1448-1450,共3页
A new anthraquinone glycoside, emodin-1-O-β-gentiobioside 1, together with threeknown compounds, chrysophanol-1-O-β-gentiobioside 2, physcion-8-O-β-gentiobioside 3, andchrysophanol-1-O-β-D-glucopyranosyl-(1→3)-β... A new anthraquinone glycoside, emodin-1-O-β-gentiobioside 1, together with threeknown compounds, chrysophanol-1-O-β-gentiobioside 2, physcion-8-O-β-gentiobioside 3, andchrysophanol-1-O-β-D-glucopyranosyl-(1→3)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranoside4 was isolated from the seeds of Cassia obtusifolia. Its structure was elucidated on the basis ofspectroscopic evidence. 展开更多
关键词 Cassia obtusifolia LEGUMINOSAE anthraqinone glycosides emodin-1-Oβ-gentiobioside.
下载PDF
Two new glycosides from the seeds of Cassia obtusifolia 被引量:5
4
作者 Cun Zhang Gui Liu Li Yong Qing Xiao Li Li Zhen Pang 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第9期1097-1099,共3页
Two new glycosides, nor-rubrofusadn-6-O-β-D-(6'-O-acetyl)glucopyranoside (1) and 1-desmethylaurantio-obtusin-2-O-β-D- glucopyranoside (2) were isolated from the seeds of Cassia obtusifolia and their structure... Two new glycosides, nor-rubrofusadn-6-O-β-D-(6'-O-acetyl)glucopyranoside (1) and 1-desmethylaurantio-obtusin-2-O-β-D- glucopyranoside (2) were isolated from the seeds of Cassia obtusifolia and their structures were established by chemical and spectral evidences. 2009 Yong Qing Xiao. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. 展开更多
关键词 Cassia obtusifolia Nor-rubrofusarin-6-O-β-D-(6'-O-acetyl)glucopyranoside 1-Desmethylaurantio-obtusin-2-O-β-D-glucopyranoside
下载PDF
Effects of GA3 and NAA on Fatty Acid Degradation in Germination and Seedling Growth of Cassia obtusifolia L. Seed 被引量:1
5
作者 Die FU Shiqiong LUO +2 位作者 Zhannan YANG Yuanshuai WANG Haitao YE 《Medicinal Plant》 CAS 2020年第1期82-87,共6页
[Objectives]To understand the content changes of fatty acid compositions and seedling growths during seed germinations of Cassia obtusifolia L.[Methods]The fatty acid compositions of germinations and seedling growths ... [Objectives]To understand the content changes of fatty acid compositions and seedling growths during seed germinations of Cassia obtusifolia L.[Methods]The fatty acid compositions of germinations and seedling growths of C.obtusifolia seeds treated with different concentrations of gibberellin(GA3)and naphthalene acetic acid(NAA)were analyzed using gas chromatograph-mass spectrometer(GC-MS),and the germination rates,root lengths,plant heights and fresh weights of their seedlings were analyzed.[Results]The contents of eicosenoic acid,α-linoleic acid,arachidic acid,eicosadienoic acid and eicosapentaenoic acid of C.obtusifolia seeds treated with different concentrations of GA3 and NAA decreased with germination time.There were significant differences between the ratios of germinations of C.obtusifolia seeds treated with different concentrations of GA3 and NAA.GA3 and NAA of different ratio treatments could promote seedling heights and fresh weights of C.obtusifolia,but increases of seedling heights and fresh weights were different.Eicosenoic acid,α-linoleic acid,arachidic acid,eicosadienoic acid and eicosapentaenoic acid were remarkably negative correlation with seedling heights and fresh weights.[Conclusions]The fatty acid components of C.obtusifolia seed germination and growth of their seedlings were affected.It indicated that GA3 and NAA treatments can affect fatty acid metabolism,the germination rates,root lengths,plant heights and fresh weights of C.obtusifolia.It is intended to provide a theoretical basis and technological reference for standardized cultivated techniques of C.obtusifolia. 展开更多
关键词 CASSIA obtusifolia L. SEED GERMINATION GERMINATION PERCENTAGE Plant growth regulator FATTY acid
下载PDF
Modeling of the Leaf Area of <i>Maytenus obtusifolia</i>Mart. from Scanned Images 被引量:1
6
作者 Vinicius de Souza Oliveira Karina Tiemi Hassuda dos Santos +8 位作者 Ana Paula Braido Pinheiro Gleyce Pereira Santos Jéssica Sayuri Hassuda Santos Hérica Chisté Omar Schmildt Sara Dousseau Arantes Marcio Paulo Czepak Adriano Alves Fernandes Edilson Romais Schmildt 《Agricultural Sciences》 2019年第6期796-806,共11页
The leaf has a vital role in the functions of the plant, being responsible for photosynthesis and gas exchange. Thus, the objective of this study was to fit a mathematical equation model to estimate the leaf area of M... The leaf has a vital role in the functions of the plant, being responsible for photosynthesis and gas exchange. Thus, the objective of this study was to fit a mathematical equation model to estimate the leaf area of Maytenus obtusifolia Mart. through the linear dimensions of the leaves. For that, six hundred and fifteen healthy leaves were collected from plants belonging to the Federal University of Espírito Santo, S&#227;o Mateus Campus, in the municipality of S&#227;o Mateus, located in the north of the State of Espírito Santo, Brazil. All leaves were digitized and the images processed using the ImageJ&#174;software, obtaining the measurements of the maximum length of the main midrib (L), the maximum width of the leaf blade (W) and the real leaf area (RLA) of each sheet. Subsequently, the product of length and width multiplication (LW) was also obtained. 500 sheets were randomly separated for the generation of models of mathematical equations and their respective coefficient of determination (R2), where RLA was used as dependent variable as function of L, W or LW as independent variable. Based on the models generated, a 115 leaf sample was used for validation, where the L, W and LW values of this sample were replaced in the adjusted equations, thus obtaining the estimated leaf area (ELA). A comparison of the means of RLA and ELA was performed by Student’s t test at 5% probability. We also calculated the mean absolute error (MAE), the root mean square error (RMSE) and the Willmott index (d). The best equation was defined by the following criteria: non-significant values of RLA and ELA averages, R2 and index d closest to unit, and MAE and RMSE values with greater proximity to zero. The quadratic model equation represented by ELA=0.18122798+0.72847767(LW)+0.00002789(LW)2 generated by multiplying the length with the width (LW) is the most suitable for the estimation of the leaf area of Maytenus obtusifolia Mart., in a fast, safe and non-destructive way. 展开更多
关键词 MAYTENUS obtusifolia MART NON-DESTRUCTIVE Method Mathematical EQUATIONS
下载PDF
Effect of EuCl_3 on Hairy Root Growth of Cassia Obtusifolia and Production of Its Active Principle
7
作者 CHANG Zhen zhan 1, LIU Yun hua 2, LU Kuan ke 3, GUO De an 3, SHEN Xin 4, WANG Sha sheng 4, WANG Kui 3( 1. Department of Biochemistry & Molecular Biology Beijing Medical University, Beijing 100083, China 2. Department of Biology, Heiloujiang 《Journal of Rare Earths》 SCIE EI CAS CSCD 2000年第2期160-160,共1页
Treated with extremely low EuCl 3 (0 001, 0 01, 1 0 mg·L -1 ) in MS medium, the hairy root growth of Cassia obtusifolia was inhibited. The inhibition was strongest at the treatment with 0 001 mg... Treated with extremely low EuCl 3 (0 001, 0 01, 1 0 mg·L -1 ) in MS medium, the hairy root growth of Cassia obtusifolia was inhibited. The inhibition was strongest at the treatment with 0 001 mg·L -1 EuCl 3, and the dry weight of hairy roots was 22% lower than that of control. Treated with rather low EuCl 3 (10 mg·L -1 ) in Murashige and Skoog(MS) medium, the hairy root growth was enhanced, and the dry weight of hairy roots was 27% higher than that of control. In the each treatment with 0 001 to 1 0 mg·L -1 EuCl 3 in MS medium, the total content of six free anthraquinones was lower than that of control. While treated with 10 mg·L -1 EuCl 3, the total content of six free anthraquinones was 26% higher than that of control, 97% higher than the treatment of 1 0 g·L -1 EuCl 3. Treatment with 10 mg·L -1 EuCl 3 in MS medium enhances the growth of hairy root of Cassia obtusifolia and improves the total content and production of six free anthraquinones in them. 展开更多
关键词 rare earths europium chloride Cassia obtusifolia hairy root growth free anthraquinones
下载PDF
Nutritional Potential of Two Leafy Vegetables <i>Leptadenia hastata</i>Decne and <i>Senna obtusifolia</i>Link Consumed in Senegal
8
作者 Nicolas Cyrille Ayessou Mady Cissé +2 位作者 Mathieu Gueye Cheikh Ndiaye Codou Mar Diop 《Food and Nutrition Sciences》 2018年第2期77-85,共9页
This study focused on two woody leafy vegetables Leptadenia hastata Decne and Senna obtusifolia Link, commonly consumed in Senegal. Leaves were col-lected from three regions. Then, proximate analyses and micronutrient... This study focused on two woody leafy vegetables Leptadenia hastata Decne and Senna obtusifolia Link, commonly consumed in Senegal. Leaves were col-lected from three regions. Then, proximate analyses and micronutrients were carried out to evaluate their nutritional values. Results revealed that protein level of S. obtusifolia (SO) is richer (21.75%) than Leptadenia hastata (LH) (18.16%). The cellulose and carbohydrate contents of the two vegetable’s leaves are in the same order except those of LH from Widou which are less rich in cellulose (8.31%) and richest in carbohydrate (6.35%). These leaves are also good sources of various mineral elements and especially iron. Leaves of LH appear to be richer in iron and magnesium, while SO appears to be richer in calcium. Vitamin C intakes of SO leaves are better than those of LH and respectively range from 142 to 196.5 and 22.5 to 159.5 mg/100 g. According to the use of this leafy vegetable by the populations, a domestication opportunity is thus justified to ensure availability and accessibility of these significant sources of micronutrients. 展开更多
关键词 Leptadenia hastata SENNA obtusifolia WOODY LEAFY Vegetables NUTRITIONAL Values Domestication
下载PDF
定点突变对决明Kunitz抑制剂抑制活性的影响
9
作者 唐婕 黄斌翰 +2 位作者 李文杰 周嘉裕 廖海 《西南农业学报》 CSCD 北大核心 2023年第12期2591-2597,共7页
【目的】决明Kunitz胰蛋白酶抑制剂1(Cassia obtusifolia Kunitz trypsin inhibitor 1,CoTI1)是一类具有β-三叶草结构的功能多肽,探究定点突变对其抑制活性的影响,对CoTI1的结构研究具有重要意义。【方法】基于CoTI1结构分析,分别将位... 【目的】决明Kunitz胰蛋白酶抑制剂1(Cassia obtusifolia Kunitz trypsin inhibitor 1,CoTI1)是一类具有β-三叶草结构的功能多肽,探究定点突变对其抑制活性的影响,对CoTI1的结构研究具有重要意义。【方法】基于CoTI1结构分析,分别将位于顶部盖子β2和β3折叠之间的Cys44,以及位于底部β-桶β9折叠内的Tyr134和Lys135这3个位点的氨基酸残基突变为丙氨酸(Ala),将各突变体进行蛋白表达之后再检测其对牛胰蛋白酶和菜青虫类胰蛋白酶的抑制作用。【结果】与野生型CoTI1相比,CoTI1^(C44D)对牛胰蛋白酶的抑制活性升高41.00%,CoTI1^(Y134D)和CoTI1^(K135D)对牛胰蛋白酶的抑制活性分别下降45.66%和36.73%;CoTI1^(C44D)、CoTI1^(Y134D)和CoTI1^(K135D)对菜青虫类胰蛋白酶的抑制活性分别下降46.94%、49.00%和38.11%。【结论】Cys44、Tyr134和Lys135参与了β-三叶草结构的形成,是稳定CoTI1的空间结构及抑制活性的重要残基,为CoTI1的结构研究奠定了重要的理论基础。 展开更多
关键词 决明 Kunitz胰蛋白酶抑制剂1 β-三叶草结构 定点突变 抑制活性
下载PDF
缓控释肥用量对不同品种决明幼苗叶绿素含量和生理特性的影响
10
作者 赵永平 李娜 +1 位作者 朱亚 寇美玉 《陕西农业科学》 2023年第5期32-37,共6页
为了明确缓控释肥用量对不同品种决明幼苗生长发育的影响,以3个决明栽培种为材料,研究了不同缓控释肥用量对决明幼苗叶绿素含量、保护酶活性、丙二醛含量、可溶性糖和可溶性蛋白含量的影响。结果表明不同缓控释肥处理间决明的叶绿素含... 为了明确缓控释肥用量对不同品种决明幼苗生长发育的影响,以3个决明栽培种为材料,研究了不同缓控释肥用量对决明幼苗叶绿素含量、保护酶活性、丙二醛含量、可溶性糖和可溶性蛋白含量的影响。结果表明不同缓控释肥处理间决明的叶绿素含量、保护酶活性、MDA含量、可溶性糖和可溶性蛋白含量均存在显著差异,品种SY-Q-2的叶绿素含量、SOD和POD活性均随缓控释肥用量的增加而升高,缓控释肥用量达到27~36 kg/hm^(2)时均达到峰值,而SY-01-02和HQ-W1-01在缓控释肥用量为18~27 kg/hm^(2)时达到最大。综合分析得出,参试的三个决明品种对缓控释肥的敏感程度总体表现为SY-Q-2〉SY-01-02〉HQ-W1-01,缓控释肥用量为27 kg/hm^(2)有利于促进决明生长发育。 展开更多
关键词 缓控释肥 决明 叶绿素含量 保护性酶
下载PDF
不同盐胁迫对决明种子萌发及幼苗生长的影响
11
作者 吴亦凤 胡倚曼 +1 位作者 闫翠香 陈纪科 《安徽农学通报》 2023年第23期30-36,共7页
为探究不同盐胁迫下决明种子萌发及幼苗生长情况,以决明种子为试材,采用不同浓度的NaCl、Na_(2)CO_(3)、Na_(2)SO_(4)溶液以及NaCl和Na_(2)CO_(3)的混合液,Na_(2)SO_(4)和Na_(2)CO_(3)的混合液对种子进行盐胁迫处理,并测定种子的发芽率... 为探究不同盐胁迫下决明种子萌发及幼苗生长情况,以决明种子为试材,采用不同浓度的NaCl、Na_(2)CO_(3)、Na_(2)SO_(4)溶液以及NaCl和Na_(2)CO_(3)的混合液,Na_(2)SO_(4)和Na_(2)CO_(3)的混合液对种子进行盐胁迫处理,并测定种子的发芽率、发芽势、发芽指数、活力指数以及幼苗根长和苗高等指标。结果表明,随着NaCl浓度的增加,决明种子各萌发指标及幼苗的生长逐渐呈下降趋势,决明种子可以适应低浓度盐分环境,但适应能力随胁迫水平的增加而降低;随着Na_(2)CO_(3)、Na_(2)SO_(4)溶液以及NaCl和Na_(2)CO_(3)的混合液,Na_(2)SO_(4)和Na_(2)CO_(3)的混合液浓度的增加,种子各萌发指标及幼苗苗高呈现先增加后下降趋势,根系生长呈下降趋势,低浓度盐溶液对决明种子的萌发有一定的促进作用。决明种子对Na+、高pH和Cl-胁迫较为敏感,不同盐分类型对决明种子的胁迫强度顺序为NaCl+Na_(2)CO_(3)>Na_(2)CO_(3)>NaCl>Na_(2)SO_(4)>Na_(2)SO_(4)+Na_(2)CO_(3)。 展开更多
关键词 决明 盐胁迫 种子萌发 幼苗生长
下载PDF
HPLC测定决明子中红镰霉素龙胆二糖苷的含量 被引量:10
12
作者 唐力英 王祝举 +2 位作者 邬秋萍 赫炎 黄璐琦 《中国中药杂志》 CAS CSCD 北大核心 2008年第4期366-368,共3页
目的:建立决明子中红镰霉素龙胆二糖苷的含量测定方法。方法:采用HPLC,Diamonsil C8柱(4.6mm×250mm,5μm),流动相乙腈-四氢呋喃-1%冰醋酸(18:3:79),流速1mL·min^-1,柱温30℃,检测波长278nm。结果:红镰霉素龙... 目的:建立决明子中红镰霉素龙胆二糖苷的含量测定方法。方法:采用HPLC,Diamonsil C8柱(4.6mm×250mm,5μm),流动相乙腈-四氢呋喃-1%冰醋酸(18:3:79),流速1mL·min^-1,柱温30℃,检测波长278nm。结果:红镰霉素龙胆二糖苷在0.1-0.5μg线性关系良好(r=0.9999),平均回收率101.1%(n=5),RSD 2.2%。结论:本法快速、灵敏、准确、可靠、重复性好,可用于中药决明子药材的质量控制。 展开更多
关键词 决明子 红镰霉素龙胆二糖苷 HPLC 含量测定
下载PDF
决明毛状根诱导及激素与诱导子对毛状根生长和蒽醌类化合物合成的影响 被引量:17
13
作者 杨世海 刘晓峰 +1 位作者 果德安 郑俊华 《中草药》 CAS CSCD 北大核心 2005年第5期752-756,共5页
目的研究激素与诱导子对决明毛状根生长和蒽醌类化合物合成的影响。方法用发根农杆菌LBA 9402感染决明C assia obtusif olia外植体,建立决明毛状根培养系统。结果NAA为决明毛状根培养的最适外源激素,培养基中添加0.05%NAA可使毛状根中5... 目的研究激素与诱导子对决明毛状根生长和蒽醌类化合物合成的影响。方法用发根农杆菌LBA 9402感染决明C assia obtusif olia外植体,建立决明毛状根培养系统。结果NAA为决明毛状根培养的最适外源激素,培养基中添加0.05%NAA可使毛状根中5种蒽醌类化合物总和提高4倍。黑曲霉诱导子对毛状根生长影响不大,却明显促进蒽醌类化合物的形成,其中每50 mL培养基加入2.0 mL诱导子可使蒽醌类化合物总量增加2倍多。茉莉酸(JA)对决明毛状根中蒽醌类化合物合成具有明显的促进作用,其中10μm o l/L作用最强,蒽醌类化合物总量比对照提高73%,JA对毛状根生长有抑制作用,但不太显著。结论为进一步筛选适宜的决明毛状根培养系统并调控次生代谢产物的研究奠定了基础。 展开更多
关键词 决明 毛状根 激素 蒽醌类化合物
下载PDF
HPLC测定决明子中决明子苷A,B含量 被引量:14
14
作者 刘松青 高振同 +2 位作者 杨大坚 代青 孔令冰 《中国药学杂志》 CAS CSCD 北大核心 1999年第4期267-269,共3页
目的:建立决明子中降血脂有效成分决明子苷A,B(以下简称苷A,B)的HPLC定量测定方法。方法:决明子药材经氯仿脱脂后再由甲醇提取,甲醇提取液经释释后直接进样分析;在μBondapakC18柱上,以乙腈水四氢呋... 目的:建立决明子中降血脂有效成分决明子苷A,B(以下简称苷A,B)的HPLC定量测定方法。方法:决明子药材经氯仿脱脂后再由甲醇提取,甲醇提取液经释释后直接进样分析;在μBondapakC18柱上,以乙腈水四氢呋喃冰醋酸(20∶76.5∶3.0∶0.5)为流动相,流速为1ml·min-1,检测波长为278nm,测定了样品中苷A、B含量,并采用光谱法和色谱法对A、B峰的色谱纯度进行了鉴定。结果:苷A、B的保留时间分别为12.13min和13.27min,经鉴定苷A、B峰均为单一物质色谱峰:苷A、B的标准曲线分别在0.25~1.25μg(r=0.9999)和0.125μg~0.625μg(r=0.9999)之间有较好线性关系,苷A、B的加样回收率分别为(96.83±3.53)%和(97.78±2.16)%。结论:本法专属性强,可用于决明子中决明子苷A、B的含量测定。 展开更多
关键词 决明子苷 决明子 中药 高效液相色谱法
下载PDF
决明子药材薄层色谱鉴别研究 被引量:19
15
作者 胡轶娟 万丽 +2 位作者 张加雄 刘毅 寿清耀 《时珍国医国药》 CAS CSCD 北大核心 2006年第11期2129-2129,共1页
目的用薄层色谱建立决明子药材的鉴别方法。方法采用硅胶H板,以橙黄决明素为对照品,石油醚∶丙酮(3∶1)展开,以氨蒸气显色。结果决明子色谱中,在与橙黄决明素相应的位置上,显相同颜色斑点,斑点清晰,无干扰。结论该方法简便、可行,为更... 目的用薄层色谱建立决明子药材的鉴别方法。方法采用硅胶H板,以橙黄决明素为对照品,石油醚∶丙酮(3∶1)展开,以氨蒸气显色。结果决明子色谱中,在与橙黄决明素相应的位置上,显相同颜色斑点,斑点清晰,无干扰。结论该方法简便、可行,为更好控制决明子的内在质量提供了科学依据。 展开更多
关键词 决明子 橙黄决明素 薄层色谱法
下载PDF
超声波强化提取决明子活性成分 被引量:10
16
作者 王琴 蒋林 +1 位作者 黄艳文 温其标 《食品工业科技》 CAS CSCD 北大核心 2005年第8期126-127,131,共3页
对超声波提取决明子中蒽醌类化合物的工艺条件进行了探讨,研究了料液比、乙醇浓度、浸泡时间和超声波提取时间等对蒽醌类成分提取率的影响。结果表明,最佳工艺条件为:料液比为0.005g/mL,用体积分数80%的乙醇溶液浸泡决明子3h,在400W的... 对超声波提取决明子中蒽醌类化合物的工艺条件进行了探讨,研究了料液比、乙醇浓度、浸泡时间和超声波提取时间等对蒽醌类成分提取率的影响。结果表明,最佳工艺条件为:料液比为0.005g/mL,用体积分数80%的乙醇溶液浸泡决明子3h,在400W的超声功率下提取80min后, 总蒽醌和游离蒽醌的提取率可达质量百分数0.596%和0.181%;其提取率明显比加热回流法和水煎煮法要高。 展开更多
关键词 超声波 决明子 蒽醌 萃取
下载PDF
钝叶黄檀地理种源变异研究 被引量:8
17
作者 孙永玉 李昆 +1 位作者 杨文云 李立 《林业科学研究》 CSCD 北大核心 2005年第3期296-299,共4页
对云南双江、龙陵、镇源、云县、石屏和墨江6个地理种源的钝叶黄檀在种荚长、宽、颜色、种子千粒质量、育苗发芽势、发芽率、幼苗苗高等指标的研究结果表明:不同地理种源的钝叶黄檀各项指标都有差异,同一地理种源的不同家系各项指标上... 对云南双江、龙陵、镇源、云县、石屏和墨江6个地理种源的钝叶黄檀在种荚长、宽、颜色、种子千粒质量、育苗发芽势、发芽率、幼苗苗高等指标的研究结果表明:不同地理种源的钝叶黄檀各项指标都有差异,同一地理种源的不同家系各项指标上也有明显的变异。不同地理种源的种子千粒质量、种荚内种子数量、种荚的宽度与种源地温度关系密切,与其它地理因素相关性不大。 展开更多
关键词 钝叶黄檀 种子 幼苗 地理种源变异
下载PDF
决明子降血脂作用机制研究 被引量:41
18
作者 何菊英 刘松青 +1 位作者 彭永富 陈泽莲 《中国药房》 CAS CSCD 2003年第4期202-204,共3页
目的 :探讨决明子降血脂作用机理。方法 :采用体外大鼠肝细胞培养 ,以Folin -酚试剂法测定肝细胞蛋白质含量 ,并以液体闪烁计数法测定肝细胞中合成 14C -胆固醇的量。结果 :决明子浸膏剂对 14C -胆固醇的合成有一定的阻抑作用 ,而决明子... 目的 :探讨决明子降血脂作用机理。方法 :采用体外大鼠肝细胞培养 ,以Folin -酚试剂法测定肝细胞蛋白质含量 ,并以液体闪烁计数法测定肝细胞中合成 14C -胆固醇的量。结果 :决明子浸膏剂对 14C -胆固醇的合成有一定的阻抑作用 ,而决明子苷B未出现明显的抑制合成 14C -胆固醇的作用。结论 :决明子浸膏剂降血脂作用是在一定程度上抑制了胆固醇的合成 ,而决明子苷B降血脂的主要途径不是抑制胆固醇的合成。 展开更多
关键词 决明子 降血脂作用 肝细胞培养 液体闪烁计数 实验研究
下载PDF
决明毛状根化学成分研究 被引量:8
19
作者 杨世海 郭洪祝 +1 位作者 果德安 郑俊华 《中国中药杂志》 CAS CSCD 北大核心 2006年第3期217-219,共3页
目的:研究决明毛状根的化学成分。方法:用发根农杆菌Agrobacterium rhizogenes9402菌株从决明子叶外植体上诱导产生毛状根,在MS0液体培养基中大量培养获得。并采用色谱技术和波谱手段对毛状根的化学成分进行分离鉴定。结果:从决明毛状根... 目的:研究决明毛状根的化学成分。方法:用发根农杆菌Agrobacterium rhizogenes9402菌株从决明子叶外植体上诱导产生毛状根,在MS0液体培养基中大量培养获得。并采用色谱技术和波谱手段对毛状根的化学成分进行分离鉴定。结果:从决明毛状根95%乙醇提取物的醋酸乙酯部分分离鉴定了8个化合物,分别是白桦酯酸、大黄酚、大黄素甲醚、豆甾醇、1-羟基-7-甲氧基-3-甲基蒽醌、大黄酚-8-甲醚、大黄酚-1-甲醚和芦荟大黄素。其中芦荟大黄素是首次从决明毛状根中分得。结论:决明毛状根能合成与原植物相似的化学成分。 展开更多
关键词 决明 毛状根 化学成分
下载PDF
外源5-氨基乙酰丙酸对盐胁迫下决明幼苗生理特性的影响 被引量:7
20
作者 谢英赞 何平 +3 位作者 韦品祥 刘海英 段才绪 胡兵 《广西植物》 CAS CSCD 北大核心 2013年第1期102-106,共5页
为寻找提升决明幼苗耐盐性的方法,测量了不同处理下决明幼苗叶片的光合色素、超氧阴离子、丙二醛的含量,以及保护酶系统中SOD、POD、CAT的活性。结果表明,经150mmol·L-1NaCl处理,决明幼苗叶片的光合色素含量显著降低,而超氧阴离子... 为寻找提升决明幼苗耐盐性的方法,测量了不同处理下决明幼苗叶片的光合色素、超氧阴离子、丙二醛的含量,以及保护酶系统中SOD、POD、CAT的活性。结果表明,经150mmol·L-1NaCl处理,决明幼苗叶片的光合色素含量显著降低,而超氧阴离子、丙二醛的含量以及保护酶SOD、POD、CAT的活性显著升高。不同浓度的外源5-氨基乙酰丙酸在不同程度上增加了决明幼苗叶片的叶绿素、类胡萝卜素含量,进一步增强了保护酶SOD、POD、CAT的活性,降低了超氧阴离子、丙二醛的含量。在外源5-氨基乙酰丙酸浓度为50mg·L-1时,恢复效果最为明显(80.0、196.4和114.1U·g-1),有效地缓解了盐胁迫对决明幼苗的伤害。 展开更多
关键词 决明 外源5-氨基乙酰丙酸 盐胁迫 生理特性
下载PDF
上一页 1 2 8 下一页 到第
使用帮助 返回顶部