Randomized controlled trial of transcutaneous electrical acupoint stimulation for early postoperative sleep disorders in patients with lung cancer

Objective To investigate the effects of transcutaneous electrical acupoint stimulation (TEAS) on the early sleep disorder of patients with lung cancer after operation.Methods Purposive sampling of 100 patients that was scheduled for lung cancer surgery in Tangdu Hospital of Air Force Military Medical University from March to July 2023 was conducted.Among them,84 patients were enrolled,and the participants were randomized into 2 groups (42 cases):transcutaneous electrical acupoint stimulation group (TEAS group) and control group (CON group).

Paclitaxel enhances the protective effect of myocardial ischemia preconditioning on ischemia/reperfusion injury in aged rat

Objective To investigate if paclitaxel can enhance the protective effect of myocardial ischemia preconditioning on ischemia/reperfusion injury in aged rat and explore related mechanism.Methods Primary cardiomyocytes of Sprague-Dawley rats were isolated by trypsin and divided into 5 groups(n=6 each):control group,hypoxia injury group,hypoxia preconditioning group,paclitaxel group,and paclitaxel + hypoxia preconditioning group.The structure of microtubules and the expression of hypoxia-inducible factor-1α(HIF-1α)were analyzed by immunofluorescence staining.The Langendorff isolated heart perfusion model was applied in 4 groups:hypoxia reperfusion injury group,hypoxia preconditioning group,paclitaxel group,and paclitaxel + hypoxia precon-ditioning group.Each group was further divided into elderly subgroup and adult subgroup(n=6 each).Left ventricular developed pressure and maximum rate of rise in left ventricular pressure were analyzed.Results(1)Primary cardiomyocyte experiments showed that the myocardial tubular microtubule structure in control group was intact and evenly stained;most of the microtubules in the hypoxia-injured group were absent and the tubular tissue was broken;the hypoxia-induced damage on microtubule structure was smaller in the hypoxic preconditioning group compared with the hypoxic injury group(microtubule staining was not uniform,and the lattice structure was broken,but not that obvious as in the hypoxia group);the tubular structure of the microtubules of the paclitaxel group was basically complete,and the staining was basically uniform.The integrity of tubular structure was maintained to some extent,similar to a normal microtubule structure in paclitaxel + hypoxia preconditioning group.The expression of HIF-1α in the cytoplasm and nucleus was very low in the control group,which was evidenced in both cytoplasm and nucleus in the hypoxic injury group.The expression was further increased in hypoxic preconditioning group,significant nuclear HIF-1 expression was found in the paclitaxel group,the expression was aggregated in the nucleus in the Paclitaxel + hypoxia preconditioning group.(2)In Langendorff isolated heart perfusion model,left ventricular developed pressure was similar between the elderly subgroup and the adult subgroup at the end of the infusion,after precondition,5 minutes of reperfusion,30 minutes of reperfusion,and 60 minutes of reperfusion in the hypoxic injury group(all P>0.05).In the hypoxic injury group,both the elderly subgroup and the adult subgroup had lower left ventricular developed pressure at 30 minutes of reperfusion when compared with the end of the infusion((15.63±4.88)mm Hg(1 mm Hg=0.133 kPa)vs.(95.63±22.14)mm Hg and(17.31±2.75)mm Hg vs.(91.00±9.58)mm Hg,respectively,all P<0.05).In the hypoxic preconditioning group,the adult subgroup had higher left ventricular developed pressure at 5 and 30 minutes of reperfusion when compared with the elderly subgroup((7.13±1.02)mm Hg vs.(3.75±1.06)mm Hg and(43.94±3.21)mm Hg vs.(16.31±1.54)mm Hg,respectively,all P<0.01).In the paclitaxel group,the adult subgroup had higher left ventricular developed pressure at 30 and 60 minutes of reperfusion when compared with the elderly subgroup((44.31±7.59)mm Hg vs.(5.44±1.21)mm Hg,(51.56±6.03)mm Hg vs.(22.19±5.14)mm Hg,respectively,all P<0.01).In the paclitaxel + hypoxia preconditioning group,both the elderly subgroup and the adult subgroup had lower left ventricular developed pressure at 30 minutes of reperfusion when compared with the end of the infusion((18.63±4.30)mm Hg vs.(99.94±8.23)mm Hg,P<0.01;(49.69±5.34)mm Hg vs.(95.31±5.26)mm Hg,P<0.05).Meanwhile,the adult sub-group had higher left ventricular developed pressure at 30 minutes of reperfusion when compared with the elderly subgroup((49.69±5.34)mm Hg vs.(18.63±4.33)mm Hg,P<0.01).The adult subgroup had higher change rate of maximum rate of rise in left ventricular pressure at 60 minutes of reperfusion when compared with the elderly subgroup in hypoxia preconditioning group,paclitaxel group,and paclitaxel combined hypoxia preconditioning group((62.83±3.92)% vs.(33.33±3.20)%,(44.17±2.32)% vs.(36.67±2.88)%,(72.50±2.66)% vs.(53.17±2.56)%,respectively,all P<0.01).Conclusion Paclitaxel can enhance the myocardial protective effect of myocardial ischemia pre-conditioning through stabilizing microtubules of cardiomyocytes and promoting HIF-1α localization in the nucleus.

Effect of Yes-associated protein in regulation of electroacupuncture pretreatment on cerebral ischemia reperfusion injury rats

Objective To observe the effect of electroacupuncture(EA)pretreatment on inflammatory reaction,apoptosis and expression of Yes-associated protein(YAP)of ischemic penumbra of cerebral cortex in cerebral ischemia reperfusion injury rats,and to explore the possible mechanism of its neuroprotection effect.

Effect of electroacupuncture at Neiguan(PC 6)on pulmonary function during one-lung ventilation in patients with lobectomy

Objective To observe the protective effect of electroacupuncture(EA)at Neiguan(PC 6)on pulmonary function during one-lung ventilation(OLV)in patients with lobectomy,and explore its action mechanism.Methods Sixty patients with lobectomy were randomly divided into an observation group and a control group,30 cases in each one.