Photometry was employed to study the optimum extraction conditions of anthraquinone derivatives from rhizomes of Rheum officinale Baill in this study.The influences of extraction solvents(chloroform,benzene,ethanol,me...Photometry was employed to study the optimum extraction conditions of anthraquinone derivatives from rhizomes of Rheum officinale Baill in this study.The influences of extraction solvents(chloroform,benzene,ethanol,methanol,and glycerol),acid,and extraction time on the extraction yield were discussed.The results indicate that,to the Rhubarb rhizomes powder with the average particle size 0.18 mm,the conditions of the extraction solvent composed by chloroform,glycerol,and sulfuric acid(20%)in the ratio of 4:1:1(v:v),the weight of dried Rhubarb to the solvent volume in the ratio of 1:12 ew:vT,extraction time of 110 min,the anthraquinone derivatives extraction could achieve the best yield.And the antibacterial tests showed the raw extraction products had the MIC(minimal inhibitory concentration)of 20μg=mL and 30μg=mL to Staphylococcus aureus and Escherichia coli,respectively.展开更多
AIM: To evaluate the capacity of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) for detecting multiple primary cancer of upper gastrointestinal (UGI) tract. METHODS: Fifteen p...AIM: To evaluate the capacity of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) for detecting multiple primary cancer of upper gastrointestinal (UGI) tract. METHODS: Fifteen patients (12 without cancer histories and 3 with histories of upper GI tract cancer) were investigated due to the suspicion of primary cancer of UGI tract on X-ray barium meal and CT scan. Subsequent whole body 18F-FDG PET/CT scan was carried out for initial staging or restaging. All the patients were fi nally confi rmed by endoscopic biopsy or surgery. The detection rate of multiple primary malignant cancers was calculated based on 18F-FDG PET/CT and endoscopic examinations.RESULTS: 18F-FDG PET/CT scan was positive in 32 suspicious lesions, 30/32 were true positive primary lesions, and 2/32 were false positive. In 15 suspicious lesions with negative 18F-FDG PET/CT scan, 12/15 were true negative and 3/15 were false negative. Among the 15 patients, 12 patients had 29 primary synchronous tumors confirmed by pathology, including 8 cases of esophageal cancers accompanied with gastric cancer and 4 of hypopharynx cancers with esophageal cancer. The other 3 patients had 4 new primary metachronous tumors, which were multiple primary esophageal cancers. PET/CT imaging detected local lymph node metastases in 11 patients. Both local lymph node metastases and distant metastases were detected in 4 patients. On a per-primary lesion basis, the sensitivity, specificity, accuracy, negative predictive value and positive predictive value of 18F-FDG PET/CT for detecting multiple primary cancer of UGI tract were 90.9%, 85.7%, 89.4%, 80% and 93.7%, respectively. CONCLUSION: The whole body 18F-FDG PET/CT may play an important role in evaluating the multiple primary malignant tumors of UGI tract cancer.展开更多
Functional research on biological macromolecules must fcus on specific loca regions.PDBlocal is aweb-based tool developed to overcome the limitations of traditional molecular visualization tools forthre-dimensional(3D...Functional research on biological macromolecules must fcus on specific loca regions.PDBlocal is aweb-based tool developed to overcome the limitations of traditional molecular visualization tools forthre-dimensional(3D)inspection of local regions.PDBlocal provides an intuitive and easy-to-manipulate web page interface and some new useful functions.It can kep loca regions flashing,display sequence text that is dynamically consistent with the 3D structure in local appearance undermultiple local manipulations,use two scenes to help users inspect the same local region withdifferent statuses,list all historical manipulation statuses with a tree structure,llow users toannotate regions ofinterest,and save ll historical statuses and other data to a web server for futureresearch.PDBlocal has met expectations and shown satisfactory performance for both expert andnovice users.This tool is available at http:/labsystem.scuec.edu.cn/pdblocal/.展开更多
In this paper,amino capped CdSe/ZnS quantum dots(QDs)were immobilized on the 11-mercaptoundecanoic acid(MUA)self-assembled Au surface(SAM/Au)by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDC).Atomic f...In this paper,amino capped CdSe/ZnS quantum dots(QDs)were immobilized on the 11-mercaptoundecanoic acid(MUA)self-assembled Au surface(SAM/Au)by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDC).Atomic force microscopy(AFM),fluorescence imaging and electrochemistry were employed to characterize the surface.The results showed that CdSe/ZnS QDs were immobilized on the surface of SAM/Au successfully.Based on this method,the fluorescence of the QDs on the SAM/Au was monitored on-line.展开更多
A series of benzo[1,2-f:5,4-f']diquinoline derivatives were synthesized starting from 2,7-diaminoanthracene, and their structures were determined by NMR, MS spectra and X-ray analysis. They showed a remarkably stron...A series of benzo[1,2-f:5,4-f']diquinoline derivatives were synthesized starting from 2,7-diaminoanthracene, and their structures were determined by NMR, MS spectra and X-ray analysis. They showed a remarkably strong intramolecular C--H...O hydrogen bond, which resulted in an unexpected downfield chemical shift of the aromatic proton in CDCl3. Moreover, a significant fluorescent change of molecule lb in the presence of trifluoroacetic acid (TFA) was also observed.展开更多
To regulate cell behaviors and promote nerve function recovery,three-dimensional(3D)conductive hydrogel can transmit intercellular electrical signals,and effectively provide the cell survival environment.However,produ...To regulate cell behaviors and promote nerve function recovery,three-dimensional(3D)conductive hydrogel can transmit intercellular electrical signals,and effectively provide the cell survival environment.However,produc-ing hydrogels with simultaneous high conductivity,favorable biocompatibility,and tissue-matching properties remains a challenge for spinal cord injury(SCI)treatment.Here,a conductive,multifunctional,and biocompati-ble VPImBF4 ionic liquid(IL)with photosensitive chitosan-based hydrogel(pCM@IL)is developed.The pCM@IL hydrogel exhibits a 3D microporous structure that could maintain cell viability and improve cell growth.Elas-tic modulus,conductivity,and biodegradability of the pCM@IL hydrogels are investigated with tissue-matching mechanical properties.The pCM@IL conductive hydrogels synergistically enhance neuronal cell proliferation and promote neuronal cells differentiation via upregulates synapse gene(Tubulin𝛽3,GAP43,Synaptophysin)expression.Furthermore,in vivo studies of the pCM@IL conductive hydrogels as implants demonstrate low-inflammation and neovascularize promotion and appropriate biodegradable properties.The developed pCM@IL conductive hydrogel is a promising therapeutic scaffold biomaterial for SCI repair.展开更多
文摘Photometry was employed to study the optimum extraction conditions of anthraquinone derivatives from rhizomes of Rheum officinale Baill in this study.The influences of extraction solvents(chloroform,benzene,ethanol,methanol,and glycerol),acid,and extraction time on the extraction yield were discussed.The results indicate that,to the Rhubarb rhizomes powder with the average particle size 0.18 mm,the conditions of the extraction solvent composed by chloroform,glycerol,and sulfuric acid(20%)in the ratio of 4:1:1(v:v),the weight of dried Rhubarb to the solvent volume in the ratio of 1:12 ew:vT,extraction time of 110 min,the anthraquinone derivatives extraction could achieve the best yield.And the antibacterial tests showed the raw extraction products had the MIC(minimal inhibitory concentration)of 20μg=mL and 30μg=mL to Staphylococcus aureus and Escherichia coli,respectively.
基金Supported by The Fund from the Bureau of Public Health of Xiamen City for creative research by young scientists,No.WQK0704the fund from the Health Department of Fujian Province for young scholars,No.2008-1-49
文摘AIM: To evaluate the capacity of 18F-fluorodeoxyglucose positron emission tomography/computed tomography (18F-FDG PET/CT) for detecting multiple primary cancer of upper gastrointestinal (UGI) tract. METHODS: Fifteen patients (12 without cancer histories and 3 with histories of upper GI tract cancer) were investigated due to the suspicion of primary cancer of UGI tract on X-ray barium meal and CT scan. Subsequent whole body 18F-FDG PET/CT scan was carried out for initial staging or restaging. All the patients were fi nally confi rmed by endoscopic biopsy or surgery. The detection rate of multiple primary malignant cancers was calculated based on 18F-FDG PET/CT and endoscopic examinations.RESULTS: 18F-FDG PET/CT scan was positive in 32 suspicious lesions, 30/32 were true positive primary lesions, and 2/32 were false positive. In 15 suspicious lesions with negative 18F-FDG PET/CT scan, 12/15 were true negative and 3/15 were false negative. Among the 15 patients, 12 patients had 29 primary synchronous tumors confirmed by pathology, including 8 cases of esophageal cancers accompanied with gastric cancer and 4 of hypopharynx cancers with esophageal cancer. The other 3 patients had 4 new primary metachronous tumors, which were multiple primary esophageal cancers. PET/CT imaging detected local lymph node metastases in 11 patients. Both local lymph node metastases and distant metastases were detected in 4 patients. On a per-primary lesion basis, the sensitivity, specificity, accuracy, negative predictive value and positive predictive value of 18F-FDG PET/CT for detecting multiple primary cancer of UGI tract were 90.9%, 85.7%, 89.4%, 80% and 93.7%, respectively. CONCLUSION: The whole body 18F-FDG PET/CT may play an important role in evaluating the multiple primary malignant tumors of UGI tract cancer.
基金supported by the International Science and Technology Cooperation Key Projects of the Chinese Ministry of Science and Technology(2009DFB30340)National Major Projects of China(2016ZX08010004-004)+2 种基金National Natural Sciences Foundation of China(81373379)Natural Science Foundation of Hubei Province of China(2012FFB07402)Fundamental Research Funds for the Central Universities in China(SouthCentral University for Nationalities,CZY12004).
文摘Functional research on biological macromolecules must fcus on specific loca regions.PDBlocal is aweb-based tool developed to overcome the limitations of traditional molecular visualization tools forthre-dimensional(3D)inspection of local regions.PDBlocal provides an intuitive and easy-to-manipulate web page interface and some new useful functions.It can kep loca regions flashing,display sequence text that is dynamically consistent with the 3D structure in local appearance undermultiple local manipulations,use two scenes to help users inspect the same local region withdifferent statuses,list all historical manipulation statuses with a tree structure,llow users toannotate regions ofinterest,and save ll historical statuses and other data to a web server for futureresearch.PDBlocal has met expectations and shown satisfactory performance for both expert andnovice users.This tool is available at http:/labsystem.scuec.edu.cn/pdblocal/.
文摘In this paper,amino capped CdSe/ZnS quantum dots(QDs)were immobilized on the 11-mercaptoundecanoic acid(MUA)self-assembled Au surface(SAM/Au)by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride(EDC).Atomic force microscopy(AFM),fluorescence imaging and electrochemistry were employed to characterize the surface.The results showed that CdSe/ZnS QDs were immobilized on the surface of SAM/Au successfully.Based on this method,the fluorescence of the QDs on the SAM/Au was monitored on-line.
基金Project supported by the National Natural Science Foundation of China (Nos. 20625206, 20902031) and the National Basic Research Program (No. 2011CB932501).
文摘A series of benzo[1,2-f:5,4-f']diquinoline derivatives were synthesized starting from 2,7-diaminoanthracene, and their structures were determined by NMR, MS spectra and X-ray analysis. They showed a remarkably strong intramolecular C--H...O hydrogen bond, which resulted in an unexpected downfield chemical shift of the aromatic proton in CDCl3. Moreover, a significant fluorescent change of molecule lb in the presence of trifluoroacetic acid (TFA) was also observed.
基金supported by National Key Research and Development Program of China(No.2016YFC1101302)self-determined and innovative research funds of WUT(S202010497265)+1 种基金A.K.Y.acknowledges the Royal Soci-ety Research Grant(RGS\R2\202305)G.Y.and N.J.acknowledge the Fundamental Research Funds for the Central Universities.
文摘To regulate cell behaviors and promote nerve function recovery,three-dimensional(3D)conductive hydrogel can transmit intercellular electrical signals,and effectively provide the cell survival environment.However,produc-ing hydrogels with simultaneous high conductivity,favorable biocompatibility,and tissue-matching properties remains a challenge for spinal cord injury(SCI)treatment.Here,a conductive,multifunctional,and biocompati-ble VPImBF4 ionic liquid(IL)with photosensitive chitosan-based hydrogel(pCM@IL)is developed.The pCM@IL hydrogel exhibits a 3D microporous structure that could maintain cell viability and improve cell growth.Elas-tic modulus,conductivity,and biodegradability of the pCM@IL hydrogels are investigated with tissue-matching mechanical properties.The pCM@IL conductive hydrogels synergistically enhance neuronal cell proliferation and promote neuronal cells differentiation via upregulates synapse gene(Tubulin𝛽3,GAP43,Synaptophysin)expression.Furthermore,in vivo studies of the pCM@IL conductive hydrogels as implants demonstrate low-inflammation and neovascularize promotion and appropriate biodegradable properties.The developed pCM@IL conductive hydrogel is a promising therapeutic scaffold biomaterial for SCI repair.