Reactive oxygen species(ROS)is widespread in living organisms.Under normal conditions,ROS remains at low concentrations to maintain normal cell function.Studies have found that the abnormal elevation of ROS levels in ...Reactive oxygen species(ROS)is widespread in living organisms.Under normal conditions,ROS remains at low concentrations to maintain normal cell function.Studies have found that the abnormal elevation of ROS levels in normal tissues is an important mechanism underlying the tumorigenesis.However,high concentrations of ROS also have toxic,genotoxic,and even apoptotic effects on tumor cells.ROS has become one of the important targets for cancer prevention and treatment.Natural products widely exist in nature and have a wide range of pharmacological activities,which can affect the function of ROS.In the present study,we reviewed the research progress of natural products in tumor prevention and treatment by regulating ROS level in recent years.展开更多
目的探讨甲状腺癌患者血浆p16基因启动子区域异常甲基化及其在甲状腺癌筛查及早期诊断中的价值,为甲状腺癌的早期诊断提供依据。方法利用巢式甲基化特异性聚合酶链反应(nested methylation specific PCR,nMSP)法检测50例甲状腺癌患者和3...目的探讨甲状腺癌患者血浆p16基因启动子区域异常甲基化及其在甲状腺癌筛查及早期诊断中的价值,为甲状腺癌的早期诊断提供依据。方法利用巢式甲基化特异性聚合酶链反应(nested methylation specific PCR,nMSP)法检测50例甲状腺癌患者和32例结节性甲状腺肿患者组织和血浆中p16基因启动子区域的异常甲基化情况;分析p16基因异常甲基化与甲状腺癌临床特征的相关性及血浆p16基因异常甲基化与肿瘤组织检测结果之间的一致性。结果甲状腺癌患者组织中p16基因启动子区甲基化率为54%(27/50),血浆标本中p16基因启动子区甲基化率为52%(26/50),两者有很好的一致性(P>0.05);结节性甲状腺肿患者组织标本和血浆标本中均未见p16基因启动子区甲基化,与甲状腺癌患者相比,差异均有统计学意义(P<0.01);血浆标本p16基因甲基化检测敏感度为52%,特异度为100%。结论血浆中p16基因启动子区域异常甲基化的检测有望成为甲状腺癌筛查和早期诊断的有效指标。展开更多
基金National Natural Science Foundation of China (Grant No. 81602729)。
文摘Reactive oxygen species(ROS)is widespread in living organisms.Under normal conditions,ROS remains at low concentrations to maintain normal cell function.Studies have found that the abnormal elevation of ROS levels in normal tissues is an important mechanism underlying the tumorigenesis.However,high concentrations of ROS also have toxic,genotoxic,and even apoptotic effects on tumor cells.ROS has become one of the important targets for cancer prevention and treatment.Natural products widely exist in nature and have a wide range of pharmacological activities,which can affect the function of ROS.In the present study,we reviewed the research progress of natural products in tumor prevention and treatment by regulating ROS level in recent years.
文摘目的探讨甲状腺癌患者血浆p16基因启动子区域异常甲基化及其在甲状腺癌筛查及早期诊断中的价值,为甲状腺癌的早期诊断提供依据。方法利用巢式甲基化特异性聚合酶链反应(nested methylation specific PCR,nMSP)法检测50例甲状腺癌患者和32例结节性甲状腺肿患者组织和血浆中p16基因启动子区域的异常甲基化情况;分析p16基因异常甲基化与甲状腺癌临床特征的相关性及血浆p16基因异常甲基化与肿瘤组织检测结果之间的一致性。结果甲状腺癌患者组织中p16基因启动子区甲基化率为54%(27/50),血浆标本中p16基因启动子区甲基化率为52%(26/50),两者有很好的一致性(P>0.05);结节性甲状腺肿患者组织标本和血浆标本中均未见p16基因启动子区甲基化,与甲状腺癌患者相比,差异均有统计学意义(P<0.01);血浆标本p16基因甲基化检测敏感度为52%,特异度为100%。结论血浆中p16基因启动子区域异常甲基化的检测有望成为甲状腺癌筛查和早期诊断的有效指标。
