为了提高预测温室黄瓜产量的能力,该研究根据温室黄瓜(品种为:戴多星Cucumis sativus cv Deltestar)果实对温度和辐射的响应,建立了以辐热积(Product of thermal effectiveness and PAR,TEP)为尺度的温室黄瓜果实模型,并用独立的试验数...为了提高预测温室黄瓜产量的能力,该研究根据温室黄瓜(品种为:戴多星Cucumis sativus cv Deltestar)果实对温度和辐射的响应,建立了以辐热积(Product of thermal effectiveness and PAR,TEP)为尺度的温室黄瓜果实模型,并用独立的试验数据进行了检验。模型对温室黄瓜各节位果实果长、果径和鲜质量的模拟值与实测值的符合度较好,模型对温室黄瓜果长和果径的模拟值与实测值之间的决定系数(R2)分别为0.7325和0.5885;回归标准误差(RMSE)分别为1.64cm和0.35cm,而以有效积温(Growing degree days,GDD)为尺度构建的果实生长模型对果长和果径的预测结果与实测值之间的决定系数(R2)分别为0.5768和0.4893;回归标准误差(RMSE)分别为1.83cm和0.40cm;本模型对果实鲜质量的模拟结果与实测值之间的回归标准误差(RMSE)和决定系数(R2)分别为25.04g和0.6782。而基于有效积温的果实生长模型对果实鲜质量的模拟结果与实测值之间的回归标准误差(RMSE)和决定系数(R2)分别为28.52g和0.6068。模拟精度提高了12.21%。本研究建立的辐热积模型能较准确地预测温室黄瓜各节位的果实生长,模型的实用性较强,可以为温室黄瓜生产提供理论依据和决策支持。展开更多
The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragment...The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragments of 141～160 and 21～40 peptide epitopes of a strain of type O FMDV was chemically synthesized and arranged into a tandem repeat 141～160 (20AA)\|21～40 (20AA)\|141～160 (20AA).This tandem sequence was fused to the 3′ end of the heavy chain constant region gene of swine immunoglobulin G and was then cloned into mammalian expression vector pCDM8 to form a recombinant plasmid pCDM8FZ3.After pCDM8FZ3 was inoculated intramuscularly into guinea pigs,it elicited a neutralizing antibody response and a specific spleen T cell proliferative response,and 66% of the vaccinated animals were protected from viral challenge.Our study indicated that the heavy chain constant region of swine IgG can act as the carrier protein for FMDV peptide epitopes,and pCDM8FZ3 is a potential DNA vaccine candidate to prevent FMDV infection.展开更多
文摘The peptide of amino acids 141～160 of VP1 protein of foot\|and\|mouth disease virus (FMDV) is a major B cell epitope and the peptide of amino acids 21～40 is an important T cell epitope.In this study,the DNA fragments of 141～160 and 21～40 peptide epitopes of a strain of type O FMDV was chemically synthesized and arranged into a tandem repeat 141～160 (20AA)\|21～40 (20AA)\|141～160 (20AA).This tandem sequence was fused to the 3′ end of the heavy chain constant region gene of swine immunoglobulin G and was then cloned into mammalian expression vector pCDM8 to form a recombinant plasmid pCDM8FZ3.After pCDM8FZ3 was inoculated intramuscularly into guinea pigs,it elicited a neutralizing antibody response and a specific spleen T cell proliferative response,and 66% of the vaccinated animals were protected from viral challenge.Our study indicated that the heavy chain constant region of swine IgG can act as the carrier protein for FMDV peptide epitopes,and pCDM8FZ3 is a potential DNA vaccine candidate to prevent FMDV infection.
