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Cloning and Expression Analysis of a Human Putative Tumor Suppressor Gene Homologue from Rice
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作者 葛晓春 宗晖 +3 位作者 詹树萱 陈继超 孙崇荣 曹凯鸣 《Acta Botanica Sinica》 CSCD 2002年第5期562-566,共5页
A gene homologous to the human Putative tumor suppressor gone QM, designated OSQM1, was isolated from rice (Oryza sativa L.) genomic DNA library using through homology screening. It contained 4 exons and 3 introns, en... A gene homologous to the human Putative tumor suppressor gone QM, designated OSQM1, was isolated from rice (Oryza sativa L.) genomic DNA library using through homology screening. It contained 4 exons and 3 introns, encoding a protein of 219 amino acids with 46 basic amino acids, leading to a high isoelectric point of 11.02. Homology search showed that this gene existed in eukaryotes and highly conserved throughout eukaryotes, suggesting an essential role of this gene. Northern Not analysis showed that it was expressed in various rice organs, but at lower level in developing flower and callus tissue than in other vegetative organs. Its expression levels in roots and leaves were influenced by different environmental factors. 展开更多
关键词 tumor suppressor gene QM EUKARYOTES
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杜氏盐藻(Dunalielle salina)盐适应相关蛋白 被引量:6
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作者 徐坤 李琳 +1 位作者 欧阳光察 焦新之 《植物生理学报(0257-4829)》 CSCD 1998年第4期327-332,共6页
观察不同盐浓度培养液中生长的杜氏盐藻可溶性蛋白SDS-PAG图谱,发现高盐与低盐相比,其51kD和63kD蛋白含量高,而23kD的蛋白含量则低。高渗骤变后,51kD蛋白的含量减少,而23kD蛋白的含量增加两倍或两倍以上,骤变23h后含量增加已非常... 观察不同盐浓度培养液中生长的杜氏盐藻可溶性蛋白SDS-PAG图谱,发现高盐与低盐相比,其51kD和63kD蛋白含量高,而23kD的蛋白含量则低。高渗骤变后,51kD蛋白的含量减少,而23kD蛋白的含量增加两倍或两倍以上,骤变23h后含量增加已非常明显;低渗骤变后24h,51kD和23kD蛋白的含量变化不明显。另外,有一分子量约为26kD的蛋白在高盐下易降解。分析了这些蛋白与社氏盐藻渗透调节的可能关系。 展开更多
关键词 盐氏盐藻 渗透骤变 渗透调节 蛋白
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