采用用计算化学方法研究金丝桃素分子结构特征,并用分子动力学方法研究其与HIV蛋白酶的相互作用,探讨其可能的抗HIV病毒作用机理.结果表明,金丝桃素分子结构具有刚性特征,与HIV蛋白酶在酶的催化活性位点与ASP-A25 and ASP-B25以氢键作...采用用计算化学方法研究金丝桃素分子结构特征,并用分子动力学方法研究其与HIV蛋白酶的相互作用,探讨其可能的抗HIV病毒作用机理.结果表明,金丝桃素分子结构具有刚性特征,与HIV蛋白酶在酶的催化活性位点与ASP-A25 and ASP-B25以氢键作用相结合.展开更多
The apoE cDNA was amplified from four months old fetus liver tissue mRNA by RT PCR. It was inserted into the TOPO ○R vector, whose sequence is the same as that reported. The right apoE cDNA was inserted into pQE30 ve...The apoE cDNA was amplified from four months old fetus liver tissue mRNA by RT PCR. It was inserted into the TOPO ○R vector, whose sequence is the same as that reported. The right apoE cDNA was inserted into pQE30 vector to construct pQE30 apoE recombinant expressed plasmid, which was expressed as a histidine tag fusion protein in E.coli strain BL21(DE3) with higher level. The purified protein was gained by Ni NTA column. SDS PAGE showed that 34 kD protein was expressed. Western blot analysis showed a positive band at 34 kD.展开更多
文摘The apoE cDNA was amplified from four months old fetus liver tissue mRNA by RT PCR. It was inserted into the TOPO ○R vector, whose sequence is the same as that reported. The right apoE cDNA was inserted into pQE30 vector to construct pQE30 apoE recombinant expressed plasmid, which was expressed as a histidine tag fusion protein in E.coli strain BL21(DE3) with higher level. The purified protein was gained by Ni NTA column. SDS PAGE showed that 34 kD protein was expressed. Western blot analysis showed a positive band at 34 kD.
