Opiate dependence has become one of the most urgent problems of modernsociety. Opiate dependence involves physical and psychical dependence. Although many addicts can bedetoxified, the relapse ratio of 95% in 5 a demo...Opiate dependence has become one of the most urgent problems of modernsociety. Opiate dependence involves physical and psychical dependence. Although many addicts can bedetoxified, the relapse ratio of 95% in 5 a demonstrates that opiate psychical dependence is aproblem more troublesome. It has been reported that acute and chronic administration of L- NNA canmarkedly retard the development of tolerance to physical dependence on morphine, and suppress theabstinence syndromes precipitated by naloxone in opiate dependent rodents, and even reverse theexisting morphine tolerance. However, the effect of L-NNA on the positive reinforcement ofpsychically active substances and its possible mechanism have not yet been reported. In presentstudy, the effect of L- NNA en the psychical dependence induced by opiates was evaluated on thebasis of conditioned place preference.展开更多
目的:研究Cystatin B(CSTB)在肝癌组织中的表达及其与肝癌患者预后的相关性。方法:利用Realtime PCR和Wester n blot分别检测肝癌病人癌与癌旁组织中CSTB m R NA水平和蛋白的表达,进一步通过免疫组织化学法检测组织芯片中CSTB表达并分...目的:研究Cystatin B(CSTB)在肝癌组织中的表达及其与肝癌患者预后的相关性。方法:利用Realtime PCR和Wester n blot分别检测肝癌病人癌与癌旁组织中CSTB m R NA水平和蛋白的表达,进一步通过免疫组织化学法检测组织芯片中CSTB表达并分析其与肝癌临床特征的关系。采用KaplanMeier生存分析法分析CSTB与肝癌预后的关系。结果:在肝癌组织中CSTB m RNA水平和蛋白水平的表达明显高于癌旁(P<0.05);生存分析发现CSTB高表达肝癌患者比低表达的患者更易复发且总体生存期短(P值均小于0.05);CSTB的表达与年龄、性别、BCLC分级以及肿瘤大小差异均无统计学意义(P值均大于0.05),CSTB高表达的患者更容易形成门脉癌栓(P<0.05)。结论:CSTB在肝细胞癌组织中高表达,其表达水平与肝癌病人预后有关,有望成为肝癌预后指标。展开更多
基金ThisprojectwassupportedbyGrantofthePLALaboratoryforNewDrugEvaluation (No .96-0 2 )
文摘Opiate dependence has become one of the most urgent problems of modernsociety. Opiate dependence involves physical and psychical dependence. Although many addicts can bedetoxified, the relapse ratio of 95% in 5 a demonstrates that opiate psychical dependence is aproblem more troublesome. It has been reported that acute and chronic administration of L- NNA canmarkedly retard the development of tolerance to physical dependence on morphine, and suppress theabstinence syndromes precipitated by naloxone in opiate dependent rodents, and even reverse theexisting morphine tolerance. However, the effect of L-NNA on the positive reinforcement ofpsychically active substances and its possible mechanism have not yet been reported. In presentstudy, the effect of L- NNA en the psychical dependence induced by opiates was evaluated on thebasis of conditioned place preference.
文摘目的:研究Cystatin B(CSTB)在肝癌组织中的表达及其与肝癌患者预后的相关性。方法:利用Realtime PCR和Wester n blot分别检测肝癌病人癌与癌旁组织中CSTB m R NA水平和蛋白的表达,进一步通过免疫组织化学法检测组织芯片中CSTB表达并分析其与肝癌临床特征的关系。采用KaplanMeier生存分析法分析CSTB与肝癌预后的关系。结果:在肝癌组织中CSTB m RNA水平和蛋白水平的表达明显高于癌旁(P<0.05);生存分析发现CSTB高表达肝癌患者比低表达的患者更易复发且总体生存期短(P值均小于0.05);CSTB的表达与年龄、性别、BCLC分级以及肿瘤大小差异均无统计学意义(P值均大于0.05),CSTB高表达的患者更容易形成门脉癌栓(P<0.05)。结论:CSTB在肝细胞癌组织中高表达,其表达水平与肝癌病人预后有关,有望成为肝癌预后指标。
