Gene mutation, rearrangement and amplification are frequently observed in mammary tumors. It is interesting to study if these genetic alterations are involved in DMBA-induced mammary tumors in 172￣(Arg-Leu) mutant p...Gene mutation, rearrangement and amplification are frequently observed in mammary tumors. It is interesting to study if these genetic alterations are involved in DMBA-induced mammary tumors in 172￣(Arg-Leu) mutant p￣(53) transgenic mice. The results of this study suggest that rearrangement of PCNA and H-ras contributed to the production of mammary tumors. No apparent gene amplification, however, was observed in these mammary tumors though several fold higher overexpression of cyclin D1 vs. normal was deteeted. The higher the expression level of 172￣(Arg-Leu) mutant p￣(53) in mammary gland of transgenic mice, the later the mammary tumor appeared in these mice. The 172￣(Arg-Leu) mutant p￣(53) in these mammary tumors behaved similarly to in vitro system.展开更多
文摘Gene mutation, rearrangement and amplification are frequently observed in mammary tumors. It is interesting to study if these genetic alterations are involved in DMBA-induced mammary tumors in 172￣(Arg-Leu) mutant p￣(53) transgenic mice. The results of this study suggest that rearrangement of PCNA and H-ras contributed to the production of mammary tumors. No apparent gene amplification, however, was observed in these mammary tumors though several fold higher overexpression of cyclin D1 vs. normal was deteeted. The higher the expression level of 172￣(Arg-Leu) mutant p￣(53) in mammary gland of transgenic mice, the later the mammary tumor appeared in these mice. The 172￣(Arg-Leu) mutant p￣(53) in these mammary tumors behaved similarly to in vitro system.
