Salt is a major environmental stressor for crops.MicroRNAs(miRNAs)play a crucial role in plant response to salt stress.In this study,a comparative analysis of miRNAs between salt-tolerant and salt-sensitive jute subje...Salt is a major environmental stressor for crops.MicroRNAs(miRNAs)play a crucial role in plant response to salt stress.In this study,a comparative analysis of miRNAs between salt-tolerant and salt-sensitive jute subjected to high salinity were carried out.374 known mature miRNAs were identified and 87 potential miRNA candidates from the eight sRNA libraries were predicted.For the salt-stressed versus the control,181 miRNAs including 45 novel miRNAs were differentially expressed.More differentially expressed miRNAs were present in TC(salt tolerant)than in NY(salt sensitive).Among NY,58 and 27 differentially expressed miRNAs were discovered in leaves and roots,respectively,whereas among TC,45 and 113 differentially expressed miRNAs were discovered in leaves and roots,respectively.Two common differentially expressed miRNAs were discovered in both leaves and roots of the two genotypes.10 differentially expressed miRNAs were common across both leaves and roots only in TC,including 6 upregulated,3 downregulated and one both up and down-regulated miRNA.The results obtained in this analysis contribute to an improved understanding of abiotic-stress tolerance in jute and will be useful for breeding programs to improve jute productivity in soils with high salinity.展开更多
[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The...[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.展开更多
文摘Salt is a major environmental stressor for crops.MicroRNAs(miRNAs)play a crucial role in plant response to salt stress.In this study,a comparative analysis of miRNAs between salt-tolerant and salt-sensitive jute subjected to high salinity were carried out.374 known mature miRNAs were identified and 87 potential miRNA candidates from the eight sRNA libraries were predicted.For the salt-stressed versus the control,181 miRNAs including 45 novel miRNAs were differentially expressed.More differentially expressed miRNAs were present in TC(salt tolerant)than in NY(salt sensitive).Among NY,58 and 27 differentially expressed miRNAs were discovered in leaves and roots,respectively,whereas among TC,45 and 113 differentially expressed miRNAs were discovered in leaves and roots,respectively.Two common differentially expressed miRNAs were discovered in both leaves and roots of the two genotypes.10 differentially expressed miRNAs were common across both leaves and roots only in TC,including 6 upregulated,3 downregulated and one both up and down-regulated miRNA.The results obtained in this analysis contribute to an improved understanding of abiotic-stress tolerance in jute and will be useful for breeding programs to improve jute productivity in soils with high salinity.
文摘[Objective]This study aims to develop an efficient regeneration system for kenaf(Hibiscus cannabinus L.),which is expected to lay a basis for breeding via genetic modification and improving the fibre yield.[Method]The influence of kenaf variety,hormone for callus induction,and explant type on the regeneration was examined.[Result]The optimal variety and explant for the regeneration were K89 and cotyledon,respectively.The 6-benzylaminopurine(6-BA)was suitable for callus induction and the optimum concentration was 2 mg/L.In addition,with this cytokinin,1.7~4.0 adventitious buds were produced,and 27.7%~38.3% of the adventitious buds could grow to plants.Adventitious roots can be induced with 0.2 mg/L naphthylacetic acid(NAA)and 0.1 mg/L indole-3-acetic acid(IAA).[Conclusion]Cotyledon of K89 had huge potential for the regeneration of kenaf.
