比色法测定冬虫夏草中甘露醇的含量

比较了测定冬虫夏草中甘露醇含量的两种方法。发现天然虫草和不同品种人工虫草制成品（胶囊剂）用容量法（Ａ）测得的甘露醇含量比用比色法（Ｂ）测得的甘露醇含量要高，但天然虫草Ａ／Ｂ比值较为接近，而不同品种人工虫草制成品（胶囊剂）的 Ａ／Ｂ比值波动较大。认为用比色法测定虫草中甘露醇含量较容量法更具特异性且快速简便。

合成肽RGD和YIGSR的聚合衍生物对PG细胞侵袭重组基底膜能力的影响

目的 探讨 ＲＧＤ和 ＹＩＧＳＲ的聚合衍生物对 ＰＧ细胞侵袭重组基底膜能力的影响。方法 利用 Ｔｒａｎｓｗｅｌｌ细胞培养小室。结果 适量 ＰＧ细胞经趋化剂作用 １０ｈ，侵袭细胞已达便于统计分析的数量范围；２５～１００μｇ／ｍｌ的合成肽 ＲＧＤ、ＹＩＧＳＲ及多数衍生物与细胞作用 ４８ｈ可对抗 ＰＧ细胞对重组基底膜的侵袭。结论 高转移性人肺白细胞癌 ＰＧ细胞系是肿瘤细胞体外侵袭实验筛选有关抗肿瘤转移药物的适用细胞。其中 ＲＧＤ和 ＹＩＧＳＲ的杂叉聚合肽及 ＹＩＧＳＲ均叉聚合肽的抗肿瘤侵袭活性较强。

何首乌毒性的研究进展

目的:为正确应用何首乌提供参考。方法:以何首乌、Polygonum multiflorum为关键词检索中、英文数据库,并查阅国内外相关文献,进行总结和归纳。结果:何首乌化学成分复杂,药理作用广泛,部分成分对肝脏有一定的毒性,但剂量-时间-效应关系尚不明确。结论:在临床使用过程中,应密切关注用药者肝脏功能的变化,以减少何首乌的不良反应。建议开展进一步的科学研究,以指导其正确应用。

Xiao-Xu-Ming decoction extract alleviates LPS-induced neuroinflammation associated with down-regulating TLR4/MyD88 signaling pathway in vitro and in vivo

In the present study, we aimed to investigate the effects of Xiao-Xu-Ming decoction extract(XXM) on lipopolysaccaride(LPS)-induced neuroinflammation in vitro and in vivo. In vitro, the microglia BV2 cells were treated with 200 ng/mL LPS for 24 h to induce inflammatory responses. In vivo, mice were treated with 5 mg/kg LPS to induce inflammatory responses. The NO level was determined by Griess Reagents. The levels of IL-1β, IL-6, TNF-α and MCP-1 were determined by ELISA. The expressions of Iba-1, TLR4 and MyD88 at the protein levels were determined by Western blotting analysis. The mRNA levels of TLR4 and MyD88 were determined by real-time PCR. In vitro, XXM significantly reduced the levels of various pro-inflammatory factors, including NO, IL-1β, IL-6 and TNF-α, induced by LPS in the supernatant of BV2 cells and suppressed expressions of inflammatory proteins TLR4 and MyD88 induced by LPS in BV2 cells. In vivo, XXM significantly inhibited microglia activation, attenuated LPS-induced inflammatory factors and chemokine production, such as IL-1β, IL-6, TNF-α and MCP-1, and inhibited the expressions of inflammatory proteins including TLR4 and MyD88, in the cortex of LPS-induced mice. Our findings suggested that XXM could attenuate LPS-induced neuroinflammation via down-regulating TLR4/MyD88 signaling pathway.

Xiao-Xu-Ming decoction extract ameliorates brain injury in rats with thrombotic focal ischemic stroke and understanding possible therapeutic targets using proteomics

Ischemic stroke seriously threatens human health and quality of life.Xiao-Xu-Ming(XXM)decoction has been a classical prescription for stroke therapy.In our previous studies,we have found that XXM exerts neuroprotective effects,improves brain injury,and attenuates neuroinflammation in cerebral ischemia rats.In this study,we investigated the effects and possible mechanism of XXM on thrombotic focal cerebral ischemia.After treatment with XXM,the neurological function and motor abilities were improved,and cerebral infarction volume was significantly decreased compared with rats of thrombotic focal cerebral ischemia.Besides,the results of BBB integrity detected by EB leakage and tight junction(TJ)protein expression showed that XXM could maintain BBB integrity and improve the expressions of TJ proteins,including claudin-1,occluding,and ZO-1,in the ischemic ipsilateral cortex disrupted by thrombotic cerebral ischemia.Furthermore,proteomic techniques were used to identify the differentially expressed proteins(DEPs)in the ischemic cerebral cortex,and the results showed that 132 DEPs regulated by XXM were detected in the ischemic cerebral cortex.Bioinformatic analysis showed that these regulated proteins by XXM were mainly involved in complement and coagulation cascade,and lysosome,etc.Furthermore,there was an interaction among DEPs,including Lgals3,Ctsz,Capg,C1qa,S100a4,Grn,Hspb1,Aif1,and Anxa1,etc.In conclusion,XXM ameliorated brain injury of thrombotic focal ischemic stroke,and Lgals3,Ctsz,Capg,C1qa,S100a4,Grn,Hspb1,Aif1,and Anxa1 could help provide possible therapeutic targets of XXM for ischemic stroke and offer research direction for further research.

Network pharmacological analysis of Xiao-Xu-Ming decoction against ischemic stroke and verification of its mechanism of anti-inflammation and neurovascular protection in vivo

Stroke is a major cause of severe disability and death.Xiao-Xu-Ming decoction(XXMD)is an effective prescription for stroke and its sequelae,while its effective ingredients and mechanism are still unclear.In the present study,we aimed to explore the effective ingredients and mechanism of XXMD in treating cerebral ischemia using network pharmacology.The main chemical components and targets of 12 herbs of XXMD were obtained by the TCMSP database and analysis platform database.The active components in XXMD were screened according to oral utilization and drug-like properties.Then,the cerebral ischemia targets were obtained through GeneCards,OMIM,TTD,Diligent and Drugbank databases.We analyzed the pathophysiological processes and pathways involved in the treatment of cerebral ischemia with XXMD by using the Metascape data analysis platform.Results showed thatβ-sitosterol,kaempferol,quercetin,stigmasterol,wogonin,and catechins might be the potential core active ingredients of XXMD in the treatment of cerebral ischemia.The therapeutic effect of XXMD on stroke was mainly exerted through regulating neuroinflammatory response and neurovascular protection.Furthermore,the anti-neuroinflammation and neurovascular protection of XXMD were further confirmed using cerebral ischemia rats.Collectively,our findings revealed that the mechanism of XXMD on the treatment of cerebral ischemia was related to anti-neuroinflammation and neurovascular protection.