To create the japonica germplasm with long grain and fragrance,we edited GS3 and OsBADH2 of a japonica rice cultivar Chunjiang 151 by using CRISPR/Cas9 system for multiplex genome editing.10 long-grain fragrant japoni...To create the japonica germplasm with long grain and fragrance,we edited GS3 and OsBADH2 of a japonica rice cultivar Chunjiang 151 by using CRISPR/Cas9 system for multiplex genome editing.10 long-grain fragrant japonica rice plants without transgenic components were obtained.Compared with those of Chunjiang 151,the grain length,thousand-grain weight,and yield per plant of the edited line increased 12.20%,18.45%,and 8.31%,respectively.We created the fragrant japonica rice line with improved grain length and yield,which enriched the germplasm resource of japonica rice and provided reference for the improvement of rice quality.展开更多
文摘To create the japonica germplasm with long grain and fragrance,we edited GS3 and OsBADH2 of a japonica rice cultivar Chunjiang 151 by using CRISPR/Cas9 system for multiplex genome editing.10 long-grain fragrant japonica rice plants without transgenic components were obtained.Compared with those of Chunjiang 151,the grain length,thousand-grain weight,and yield per plant of the edited line increased 12.20%,18.45%,and 8.31%,respectively.We created the fragrant japonica rice line with improved grain length and yield,which enriched the germplasm resource of japonica rice and provided reference for the improvement of rice quality.
