We first report the effect of a compound EP-1 (mixture of levonorgestrel and quinestrol) on male greater long-tailed hamsters (Tscherskia triton). Hamsters in the treatment group were provided with wheat baits with 0....We first report the effect of a compound EP-1 (mixture of levonorgestrel and quinestrol) on male greater long-tailed hamsters (Tscherskia triton). Hamsters in the treatment group were provided with wheat baits with 0.001% EP-1 for six consecutive days, while hamsters in the control group were provided with plain wheat. One week and three weeks later, reproductive organs of hamsters of both groups were measured, respectively. We found the size or weight of testis, spermatophore and epididymis of the treatment group were significantly reduced compared to those of the control group, and this reduction was greater in the third week compared to that of the first week. The results indicate that EP-1 is able to reduce the fertility of both male and female hamsters.展开更多
The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into pBV220.The recombinant vector pBV220B1 was constructed and transformed into E.coli DH5α.A 60 kD protein was induced by 42℃ and its e...The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into pBV220.The recombinant vector pBV220B1 was constructed and transformed into E.coli DH5α.A 60 kD protein was induced by 42℃ and its expression was temperature\|dependent.After 6h induction,the target protein occupied 50% of the total protein.The expressed product existed in both inclusion body and soluble proteins in the cells.The amount of the soluble detoxifying enzyme increased along with the induction time.The data of detoxifying experiments indicated that the detoxifying enzyme in expression strain of E.coli can detoxified toxicity of organophosphate insecticides,it showed a clear detoxifying affect on hens poisoned by organophosphate insecticides.展开更多
文摘We first report the effect of a compound EP-1 (mixture of levonorgestrel and quinestrol) on male greater long-tailed hamsters (Tscherskia triton). Hamsters in the treatment group were provided with wheat baits with 0.001% EP-1 for six consecutive days, while hamsters in the control group were provided with plain wheat. One week and three weeks later, reproductive organs of hamsters of both groups were measured, respectively. We found the size or weight of testis, spermatophore and epididymis of the treatment group were significantly reduced compared to those of the control group, and this reduction was greater in the third week compared to that of the first week. The results indicate that EP-1 is able to reduce the fertility of both male and female hamsters.
文摘The full\|length cDNA of mosquito esterase B1 had been isolated,and subcloned into pBV220.The recombinant vector pBV220B1 was constructed and transformed into E.coli DH5α.A 60 kD protein was induced by 42℃ and its expression was temperature\|dependent.After 6h induction,the target protein occupied 50% of the total protein.The expressed product existed in both inclusion body and soluble proteins in the cells.The amount of the soluble detoxifying enzyme increased along with the induction time.The data of detoxifying experiments indicated that the detoxifying enzyme in expression strain of E.coli can detoxified toxicity of organophosphate insecticides,it showed a clear detoxifying affect on hens poisoned by organophosphate insecticides.
