The rapid purification(less than 1.5 h) of EPSP synthase from bean seedling by Sephadex G 50 and Mono Q chromtography was reported. Specific activity of EPSP synthase obtained by the method was 175.2 nmol·min -1 ...The rapid purification(less than 1.5 h) of EPSP synthase from bean seedling by Sephadex G 50 and Mono Q chromtography was reported. Specific activity of EPSP synthase obtained by the method was 175.2 nmol·min -1 ·mg -1 .Concentrated enzyme solution after adjusting to 50% glycerol( V/V ) and 1mg·mL -1 BSA, was stored at -20℃. EPSP synthase activity was stable at least for 150 days.The activity of EPSP synthase was inhibited approximately 50% by 6.3 μmol·L -1 glyphosate. It showed that the purified EPSP synthase as herbicide screening model is possible. This purified method has been used to study enzyme mechanism of the glyphosate resistant bean.展开更多
流化床具有良好的传热速率、较好的气固接触并能处理大量固体颗粒,在化工过程中得到了极广泛的应用,但由于床内流动的复杂性,在设备放大过程会遇到不同难点。使用数学模型进行工程放大及优化,能节约大量人力物力和财力,缩短开发周期。...流化床具有良好的传热速率、较好的气固接触并能处理大量固体颗粒,在化工过程中得到了极广泛的应用,但由于床内流动的复杂性,在设备放大过程会遇到不同难点。使用数学模型进行工程放大及优化,能节约大量人力物力和财力,缩短开发周期。流化床的数学模型分三类:无量纲模型、经典数学模型和多相流体力学模型。其中多相流体力学模型的假设和所需的经验数据少,给出的信息多,但需要的计算是非常巨大的,过去应用不广,近年来随着计算机技术的迅速发展使复杂的计算成为可能,因而在国际上越来越受到重视。我们开发成功的通用程序CASIC(Computational Analysis for Significant Information of Coal Combustion and Gasification),包含了国际上这个领域的最新成果,其计算结果与文献值或实验数据基本吻合,已显示出广阔的应用前景。展开更多
文摘The rapid purification(less than 1.5 h) of EPSP synthase from bean seedling by Sephadex G 50 and Mono Q chromtography was reported. Specific activity of EPSP synthase obtained by the method was 175.2 nmol·min -1 ·mg -1 .Concentrated enzyme solution after adjusting to 50% glycerol( V/V ) and 1mg·mL -1 BSA, was stored at -20℃. EPSP synthase activity was stable at least for 150 days.The activity of EPSP synthase was inhibited approximately 50% by 6.3 μmol·L -1 glyphosate. It showed that the purified EPSP synthase as herbicide screening model is possible. This purified method has been used to study enzyme mechanism of the glyphosate resistant bean.
文摘流化床具有良好的传热速率、较好的气固接触并能处理大量固体颗粒,在化工过程中得到了极广泛的应用,但由于床内流动的复杂性,在设备放大过程会遇到不同难点。使用数学模型进行工程放大及优化,能节约大量人力物力和财力,缩短开发周期。流化床的数学模型分三类:无量纲模型、经典数学模型和多相流体力学模型。其中多相流体力学模型的假设和所需的经验数据少,给出的信息多,但需要的计算是非常巨大的,过去应用不广,近年来随着计算机技术的迅速发展使复杂的计算成为可能,因而在国际上越来越受到重视。我们开发成功的通用程序CASIC(Computational Analysis for Significant Information of Coal Combustion and Gasification),包含了国际上这个领域的最新成果,其计算结果与文献值或实验数据基本吻合,已显示出广阔的应用前景。
