The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme w...The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9. 175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolutiorl using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewermolecules in the asymmetric unit.展开更多
电子显微成像技术的快速发展使得对完整细胞、组织乃至整个机体进行高分辨三维结构解析研究成为可能,这些可进行大尺度生物样品三维结构研究的电子显微成像技术统称为体电子显微学技术(volume electron microscopy,vEM)。近年来,v EM在...电子显微成像技术的快速发展使得对完整细胞、组织乃至整个机体进行高分辨三维结构解析研究成为可能,这些可进行大尺度生物样品三维结构研究的电子显微成像技术统称为体电子显微学技术(volume electron microscopy,vEM)。近年来,v EM在研究尺度、分辨率、吞吐量和易用性等方面发展迅速,在整个生命科学领域的应用呈爆炸式增长,该技术因此被《自然》(Nature)评为2023年最值得关注的七项前沿技术之一。然而,vEM相关技术的发展和应用在国内起步较晚,亟待进一步推广。本综述涵盖了vEM的发展历程、技术分类、样品制备、数据收集、图像处理等全方位的内容,便于生命科学、医学等领域研究人员去了解、学习、应用和进一步发展该技术。展开更多
文摘The basic phospholipase A2 from the venom of Agkistrodon halys pallas possesses the ability to cause hemolysis in contrast to the other two phospholipase A2 from the same venom. A new form of crystals of this enzyme was grown. The crystals belong to space group of P212121 with unit cell parameters of a=9. 175nm, b=10.080nm , c=2.287nm.The crystals diffract to high resolution, and are suitable for detailed structural studies. The data were collected up to 0.25nm resolutiorl using synchrotron radiation-imaging plate-Weissenberg camera system. Preliminary analysis reveals the presence of two molecules in the asymmetric uint and the molecule may pack with the smallest dimension approximately parallel to the c axis. The new crystal form is more attractive than the monoclinic one previously reported for crystallographic structure determination as it contains fewermolecules in the asymmetric unit.
文摘电子显微成像技术的快速发展使得对完整细胞、组织乃至整个机体进行高分辨三维结构解析研究成为可能,这些可进行大尺度生物样品三维结构研究的电子显微成像技术统称为体电子显微学技术(volume electron microscopy,vEM)。近年来,v EM在研究尺度、分辨率、吞吐量和易用性等方面发展迅速,在整个生命科学领域的应用呈爆炸式增长,该技术因此被《自然》(Nature)评为2023年最值得关注的七项前沿技术之一。然而,vEM相关技术的发展和应用在国内起步较晚,亟待进一步推广。本综述涵盖了vEM的发展历程、技术分类、样品制备、数据收集、图像处理等全方位的内容,便于生命科学、医学等领域研究人员去了解、学习、应用和进一步发展该技术。
