Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. ...Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. Histochemical assay of GUS activity showed positive reaction on some of the transformation processed apical points and calli. This demonstrated that foreign genes were introduced into the apical meristematic cells as well as the callus cells. The plantlets of cv. “Jing 411” survived after apical point transformation with pBIAH A + were transplanted into the field and the progenies were screened with kanamycin. 4% of the screened seeds germinated into green seedlings with kanamycin resistance. Dot hybridization of total DNA from kanamycin resistant plants showed the existence of foreign DNA in some of the detected plants.展开更多
文摘Apical points of young seedlings of wheat (Triticum aestivum) cultivar “Jing 411” and somatic calli of cultivar “FK8” were transformed with plasmids pBI121 and (or) pBIAH A + by using microprojectile bombardment. Histochemical assay of GUS activity showed positive reaction on some of the transformation processed apical points and calli. This demonstrated that foreign genes were introduced into the apical meristematic cells as well as the callus cells. The plantlets of cv. “Jing 411” survived after apical point transformation with pBIAH A + were transplanted into the field and the progenies were screened with kanamycin. 4% of the screened seeds germinated into green seedlings with kanamycin resistance. Dot hybridization of total DNA from kanamycin resistant plants showed the existence of foreign DNA in some of the detected plants.
