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Meiotic Behavior of 1BL/1RS Translocation Chromosome and Alien Chromosome in Two Tri-genera Hybrids 被引量:3
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作者 李义文 李振声 贾旭 《Acta Botanica Sinica》 CSCD 2002年第7期821-826,共6页
The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivisio... The behavior of wheat-rye translocation chromosome and alien chromosome including Thinopyrum and Haynaldia chromosome at meiosis was investigated in two hybrids by fluorescence in situ hybridization (FISH). Misdivision of translocation chromosome at anaphase I and rye chromatin micronucleus at tetrad stage were observed, A plant with one normal 1BL/1RS translocation chromosome and one 1BL/1RS translocation chromosome deleted about 1/3 of rye chromosome arm in length was identified. One plant with wheat-Thinopyrum non-Robertson translocation chromosome was also detected in the F-2 population of Yi4212 x Yi4095. That could be the results of unequal misdivision of wheat-rye 1BL/1RS translocation chromosome and Thinopyrum chromosome during meiosis. No interaction between translocation chromosome and alien chromosome at meiosis was supported by the data of the distribution frequencies of translocation chromosome and Thinopyrum or Haynaldia chromosome in the progeny of two hybrids. The results may be useful to cultivate new germplasms with different length of rye 1R short arm and wheat-alien non-Robertson translocation tines under wheat background. 展开更多
关键词 MEIOSIS chromatin univalent 1BL/1RS translocation chromosome fluorescence in situ hybridization
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Genetic Transformation in Triticeae Crops 被引量:3
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作者 李义文 徐龙源 +1 位作者 李振声 贾旭 《Acta Botanica Sinica》 CSCD 2002年第5期505-508,共4页
Wheat, triticale, tritordeum, barley, oat and rye are the most important crops in human consumptions and industry in the world. Transformation technology supplies a new source of improving Triticeae crops. In the past... Wheat, triticale, tritordeum, barley, oat and rye are the most important crops in human consumptions and industry in the world. Transformation technology supplies a new source of improving Triticeae crops. In the past decade, transformation of wheat crops has considerably progressed. Many transgenic plants of Triticeae crops with various genes were produced via nricroprojectile bombardment, Agrobacterium-mediated transformation, PEG-uptake DNA technique, electroporation, microinjection, injection inflorescence and silicone carbide. Integration and expression of transgenes, inheritance and variation of transgenic plants have been studied. Technical improvements of genetic transformation for wheat crops will be extensively useful in commerce and benefit significantly to human being in the world. 展开更多
关键词 Triticeae crops transformation techniques transgenic plant gene expression and variation
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氮磷亏缺对小麦TaIPS基因表达的影响 被引量:3
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作者 李彦龙 童依平 +3 位作者 李滨 赵惠贤 张学勇 李振声 《西北植物学报》 CAS CSCD 北大核心 2008年第7期1303-1307,共5页
为了解小麦高效利用土壤磷的分子机理和实现对小麦缺磷的分子诊断,以普通小麦(Triticum aestivum L.)小偃54为材料,克隆了5个受缺磷诱导的IPS基因,同源比较结果显示,小麦IPS基因属于典型的受缺磷条件特异诱导的TPSI1/MT4小基因家族.对... 为了解小麦高效利用土壤磷的分子机理和实现对小麦缺磷的分子诊断,以普通小麦(Triticum aestivum L.)小偃54为材料,克隆了5个受缺磷诱导的IPS基因,同源比较结果显示,小麦IPS基因属于典型的受缺磷条件特异诱导的TPSI1/MT4小基因家族.对小麦根系和地上部的半定量RT-PCR研究结果表明,与全营养处理对照相比,3叶期小麦幼苗经过缺氮、缺磷和氮磷同时缺乏处理8d后,缺磷显著增加了根系中3个TaIPS1(TaIPS1.1、TaIPS1.2和TaIPS1.3)基因和地上部TaIPS1.1基因的表达,中度上调了根系中2个TaIPS2基因(TaIPS2.1和TaIPS2.2)的表达,轻度上调了地上部TaIPS1.2和2个TaIPS2基因的表达.通过比较5个基因在根系和地上部对缺磷的响应,认为TaIPS1.1是一个较理想的用于诊断小麦植株磷素丰缺的基因.缺氮不仅降低了3个TaIPS1基因在根系中的表达,并抑制了IPS基因对缺磷的响应.这一研究结果预示了TaIPS基因对低磷胁迫的响应依赖于植株体内的氮素营养状况. 展开更多
关键词 缺氮 缺磷 TaIPS 小麦
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