家蚕质多角体病毒(BmCPV)基因组dsRNA片段V的全序列测定

dsRNA segment Ⅴ was separated from Bombyx mori cytoplasmic polyhedrosis virus(BmCPV)of Chinese Guangzhou strainAfter ligation with a ssDNA adaptor,the cDNA fragments overlapping full-length segment Ⅴ of BmCPV were acquired by RT-PCR amplificationSegment Ⅴ is 2852 nucleotides long and possesses a single open reading frame encoding a putative protein of 881 amino acidsComparison of amino acid sequences shows 97% homology with BmCPV Japanese isolate segments Ⅴ,86% and 36% with LdCPV-1 and LdCPV-14 segments Ⅴ respectivelyPart of sequence of BmCPV segment Ⅴ exhibits high homology with 2Apro motif of picornavirus members,which may suggest that there might exist some affinities evolutionally between both of

家蚕质多角体病毒片段IV的全序列测定

在随机引物建库的基础上 ,通过交叉设计引物及加单链DNA接头 ,应用RT PCR技术克隆了家蚕质多角体病毒dsRNA片段Ⅳ的全长cDNA ,并测定了它的全序列。该片段长 3,2 6 2bp ,含有一个完整的开放读码框 ,编码一个长 1 ,0 5 8氨基酸的成熟多肽。序列分析表明 ,该片段与日本BmCPV片段Ⅳ的核苷酸序列同源性为 89% ,氨基酸序列同源性为 95 %。