Sequences from a region of the nuclear ribosomal 18S rDNA gene of approximately 1 912 base pairs （bp） were used to generate a molecular phylogeny for the Pentatomomorpha based on 53 species representing 21 putative ...Sequences from a region of the nuclear ribosomal 18S rDNA gene of approximately 1 912 base pairs （bp） were used to generate a molecular phylogeny for the Pentatomomorpha based on 53 species representing 21 putative families. Phylogenetic analyses using the most parsimony method （MP）, maximum likelihood method （ML）, and neighbor joining method （NJ） showed strong support that the Pentatomomorpha lineage is a monophyly and the superfamily Aradoidea is a sister group to the remainder of the Pentatomomorpha （Trichophora）. The Trichophora could be divided into two clades ： one clade consisted of the monophyletic superfamilies Pentatomoidea and Pyrrhocoroidea; the other was mainly the polyphyletic superfamilies Lygaeoidea, Coreoidea and Idiostoloidea. The superfamilies Lygaeoidea and Coreoidea were both polyphyletic. Within Lygaeoidea, Piesmatidae was sister to Berytidae. They formed a clade locating at the basal of the Trichophora and distantly related to the other two families Lygaeidae and Rhyparochromidae. This research suggested that 18S rDNA was a proper marker to reconstruct the phylogeny of Pentatomomorpha that was accordant to morphological studies and the research of Li et al （2005）. The Pyrrhocoroidea was further divided from the Coreoidea （s./at ）. It was suggested that the Piesmatidae might be assigned as a superfamily of Pentatomomorpha rather than a family in Lygaeoidea.展开更多
Interaction between a parasite and its host could lead to a co-evolutionary arms race. Cuckoo-host system is among the most studied of all brood parasite systems, but the cuckoos of Asia, on the other hand, are much l...Interaction between a parasite and its host could lead to a co-evolutionary arms race. Cuckoo-host system is among the most studied of all brood parasite systems, but the cuckoos of Asia, on the other hand, are much less well known. China has the most abundant cuckoo species in Asia. Many of these co-occur in sympatric areas, posing a potential risk of mis-identification of cuckoo nestlings, especially in Cuculus species. In this study we have provided a practical criterion to identify cuckoo nestlings species in the field and performed molecular phylogeny to confirm our empirical results. These results indicate that two distinct characteristics of cuckoo nestlings, i.e., the gape color pattern and feather traits can be considered as reliable species identification. To our knowledge, this is the first report for species identification of Cuculus nestlings through molecular analysis.展开更多
文摘Sequences from a region of the nuclear ribosomal 18S rDNA gene of approximately 1 912 base pairs （bp） were used to generate a molecular phylogeny for the Pentatomomorpha based on 53 species representing 21 putative families. Phylogenetic analyses using the most parsimony method （MP）, maximum likelihood method （ML）, and neighbor joining method （NJ） showed strong support that the Pentatomomorpha lineage is a monophyly and the superfamily Aradoidea is a sister group to the remainder of the Pentatomomorpha （Trichophora）. The Trichophora could be divided into two clades ： one clade consisted of the monophyletic superfamilies Pentatomoidea and Pyrrhocoroidea; the other was mainly the polyphyletic superfamilies Lygaeoidea, Coreoidea and Idiostoloidea. The superfamilies Lygaeoidea and Coreoidea were both polyphyletic. Within Lygaeoidea, Piesmatidae was sister to Berytidae. They formed a clade locating at the basal of the Trichophora and distantly related to the other two families Lygaeidae and Rhyparochromidae. This research suggested that 18S rDNA was a proper marker to reconstruct the phylogeny of Pentatomomorpha that was accordant to morphological studies and the research of Li et al （2005）. The Pyrrhocoroidea was further divided from the Coreoidea （s./at ）. It was suggested that the Piesmatidae might be assigned as a superfamily of Pentatomomorpha rather than a family in Lygaeoidea.
基金supported by the National Natural Science Foundation of China (No. 31071938, 31101646)the Key Project of the Chinese Ministry of Education (No. 212136)the Program for New Century Excellent Talents in University(NCET-10-0111)
文摘Interaction between a parasite and its host could lead to a co-evolutionary arms race. Cuckoo-host system is among the most studied of all brood parasite systems, but the cuckoos of Asia, on the other hand, are much less well known. China has the most abundant cuckoo species in Asia. Many of these co-occur in sympatric areas, posing a potential risk of mis-identification of cuckoo nestlings, especially in Cuculus species. In this study we have provided a practical criterion to identify cuckoo nestlings species in the field and performed molecular phylogeny to confirm our empirical results. These results indicate that two distinct characteristics of cuckoo nestlings, i.e., the gape color pattern and feather traits can be considered as reliable species identification. To our knowledge, this is the first report for species identification of Cuculus nestlings through molecular analysis.
