利用云南文山采集的人工栽培和野生云南重楼Paris polyphylla Smith var.yunnanensis(Franch)根茎,对其内生细菌进行了分离、纯化及鉴定。分离得到40和44株云南重楼内生细菌。经16S rDNA测序,在NCBI中比对,MEGA4建系统进化树,所得内生...利用云南文山采集的人工栽培和野生云南重楼Paris polyphylla Smith var.yunnanensis(Franch)根茎,对其内生细菌进行了分离、纯化及鉴定。分离得到40和44株云南重楼内生细菌。经16S rDNA测序,在NCBI中比对,MEGA4建系统进化树,所得内生细菌分别鉴定为分属于6和8个属。其中,人工栽培云南重楼内生细菌中,芽孢杆菌属(Bacillus)和肠杆菌属(Enterobacter)分离频率分别为35%、27.5%,是其优势内生细菌群;野生云南重楼内生细菌中,芽孢杆菌属(Bacillus)和克雷伯氏菌属(Klebsiella)分离频率分别为36.4%、20.4%,是其优势内生细菌群。本研究结果体现了人工栽培和野生云南重楼内生细菌分布的差异及云南重楼根中内生细菌的多样性,也为后续重楼内生菌的多样性开发提供了理论和实验基础。展开更多
Fusarium 9568D, which belongs to Fusarium moniliforme , was isolated from the roots of Dracaena cochinchinensis in the suburb of Monglian, Yunnan Province of China. High activities of β glucosidase and cellulase were...Fusarium 9568D, which belongs to Fusarium moniliforme , was isolated from the roots of Dracaena cochinchinensis in the suburb of Monglian, Yunnan Province of China. High activities of β glucosidase and cellulase were detected in the broth of Fusarium 9568D. The strain was inoculated in abiotic branch and wood of Dracaena cochinchinensis . After 4～5 months of culture, red resin emerged in the inoculated points. UV IR spectrum analysis and antibiotic assay demonstrated that this resin almost resembled the natural blood resin.展开更多
Solid phase micro-extract (SPME) using modified pencil lead as adsorbent is applied to the analysis of the volatileorganic compounds in vinegar.and the optimum conditions for sample preparation are discussed.
文摘利用云南文山采集的人工栽培和野生云南重楼Paris polyphylla Smith var.yunnanensis(Franch)根茎,对其内生细菌进行了分离、纯化及鉴定。分离得到40和44株云南重楼内生细菌。经16S rDNA测序,在NCBI中比对,MEGA4建系统进化树,所得内生细菌分别鉴定为分属于6和8个属。其中,人工栽培云南重楼内生细菌中,芽孢杆菌属(Bacillus)和肠杆菌属(Enterobacter)分离频率分别为35%、27.5%,是其优势内生细菌群;野生云南重楼内生细菌中,芽孢杆菌属(Bacillus)和克雷伯氏菌属(Klebsiella)分离频率分别为36.4%、20.4%,是其优势内生细菌群。本研究结果体现了人工栽培和野生云南重楼内生细菌分布的差异及云南重楼根中内生细菌的多样性,也为后续重楼内生菌的多样性开发提供了理论和实验基础。
文摘Fusarium 9568D, which belongs to Fusarium moniliforme , was isolated from the roots of Dracaena cochinchinensis in the suburb of Monglian, Yunnan Province of China. High activities of β glucosidase and cellulase were detected in the broth of Fusarium 9568D. The strain was inoculated in abiotic branch and wood of Dracaena cochinchinensis . After 4～5 months of culture, red resin emerged in the inoculated points. UV IR spectrum analysis and antibiotic assay demonstrated that this resin almost resembled the natural blood resin.
文摘Solid phase micro-extract (SPME) using modified pencil lead as adsorbent is applied to the analysis of the volatileorganic compounds in vinegar.and the optimum conditions for sample preparation are discussed.
