采用诱抗剂苯并噻二唑(BTH)对滇牡丹3种主要病害(牡丹红斑病Cladosporium red spot、灰霉病Grey mould和黑斑病Black spot)进行了诱抗试验,分析了发病指数、诱抗效果、生理等相关指标变化。结果表明:诱抗剂对滇牡丹3种病害诱抗效果显著...采用诱抗剂苯并噻二唑(BTH)对滇牡丹3种主要病害(牡丹红斑病Cladosporium red spot、灰霉病Grey mould和黑斑病Black spot)进行了诱抗试验,分析了发病指数、诱抗效果、生理等相关指标变化。结果表明:诱抗剂对滇牡丹3种病害诱抗效果显著,其中以红斑病的诱抗效果最佳,并确定出BTH的最佳使用浓度为100mg/L;在此浓度处理后,叶片病斑面积停止增长,边缘逐渐愈合,SAFR和MDA含量显著降低,可溶性蛋白、色素和相关酶系活力指标均显著增加,这些变化与病害的抗性变化密切相关。诱抗剂BTH对滇牡丹3种病原菌不产生毒杀作用,而是诱导植物系统获得性抗性(SAR),其作用机理有待进一步的研究。展开更多
Inter-simple sequence repeat(ISSR) molecular markers were applied to analyze the genetic diversity and clustering of 48 introduced and bred cultivars of Olea euyopaea L. Totally 106 DNA bands were amplified by 11 sc...Inter-simple sequence repeat(ISSR) molecular markers were applied to analyze the genetic diversity and clustering of 48 introduced and bred cultivars of Olea euyopaea L. Totally 106 DNA bands were amplified by 11 screened primers, including 99 polymorphic bands; the percentage of polymorphic loci was 93.40%, indicating a rich genetic diversity in Olea euyopaea L. germplasm resources. Based on Nei's genetic distances between various cultivars, a dendrogram of 48 cultivars of Olea euyopaea L. was constructed using unweighted pair-group(UPMGA)method,which showed that 48 cultivars were clustered into four main categories; 84.6% of native cultivars were clustered into two categories; most of introduced cultivars were clustered based on their sources and main usages but not on their geographic origins. This study will provide references for the utilization and further genetic improvement of Olea euyopaea L. germplasm resources.展开更多
文摘采用诱抗剂苯并噻二唑(BTH)对滇牡丹3种主要病害(牡丹红斑病Cladosporium red spot、灰霉病Grey mould和黑斑病Black spot)进行了诱抗试验,分析了发病指数、诱抗效果、生理等相关指标变化。结果表明:诱抗剂对滇牡丹3种病害诱抗效果显著,其中以红斑病的诱抗效果最佳,并确定出BTH的最佳使用浓度为100mg/L;在此浓度处理后,叶片病斑面积停止增长,边缘逐渐愈合,SAFR和MDA含量显著降低,可溶性蛋白、色素和相关酶系活力指标均显著增加,这些变化与病害的抗性变化密切相关。诱抗剂BTH对滇牡丹3种病原菌不产生毒杀作用,而是诱导植物系统获得性抗性(SAR),其作用机理有待进一步的研究。
基金Supported by Key Project of New Product Development in Yunnan Province(2009BB006)~~
文摘Inter-simple sequence repeat(ISSR) molecular markers were applied to analyze the genetic diversity and clustering of 48 introduced and bred cultivars of Olea euyopaea L. Totally 106 DNA bands were amplified by 11 screened primers, including 99 polymorphic bands; the percentage of polymorphic loci was 93.40%, indicating a rich genetic diversity in Olea euyopaea L. germplasm resources. Based on Nei's genetic distances between various cultivars, a dendrogram of 48 cultivars of Olea euyopaea L. was constructed using unweighted pair-group(UPMGA)method,which showed that 48 cultivars were clustered into four main categories; 84.6% of native cultivars were clustered into two categories; most of introduced cultivars were clustered based on their sources and main usages but not on their geographic origins. This study will provide references for the utilization and further genetic improvement of Olea euyopaea L. germplasm resources.