中草药抗鸭乙型肝炎病毒的效果

采用鸭乙型肝炎动物模型对叶下珠、虎杖、五味子等中草药乙醇提取物进行了抗嗜肝DNA病毒效果评价。将一日龄北京鸭注乙肝病毒后随机分组,每组给予不同剂量药物,分别在给药前To、给药7天(T7).给药15天(T15)和停药后7天(P7)等不同时间,取血测定血清中DHBVDNA.对照组以生理盐水代替药物。结果显示出这些药物均有不同程度的抗DHBV治疗效果.尤以叶下珠为好.呈现出剂量反应关系;将这些药物联合作用.结果显示出比单味药有更好的治疗效果。

抗HBx IgM的检测及临床应用

利用羊抗人IgM,基因工程表达的乙型肝炎X抗原和酶标抗-HBx单克隆抗体,建立了检测血清中抗-HBx IgM充体的ELISA双夹心法,并进行了初步应用。实验结果表明,此方法的特异性强,稳定性及重复性好(批内变异系数8.64％,批间变异系数10.60％,重复率为100％),且不受血清中抗-HBc IgM、抗-HAV IgM及类风湿因子的影响。由于目前国内外都没有抗-HBx IgM标准品,故对方法的敏感性没有报道。

河南南阳蚋科昆虫的调查

1 调查生境 河南省有关蚋科昆虫的调查资料,迄今未见报道。1994年7月,在河南内乡宝天曼(33.1°N,111.7°E)自然保护区进行了蚋虫科的采集调查。该区位于伏牛山麓,海拔约100m,山上有松树等杂木。山间有一条流水,宽2～10m,深0.1～1m,水流速度为0.2～0.6m/秒,岸边丛生杂草,幼虫和蛹就生长在水中1～3cm的杂草茎叶上及浸没水中的石块下面。

Identification of Shiga-like toxin Escherichia coli isolated from children with diarrhea by polymerase chain reaction

Objective To evaluate the etiology of Shiga-like toxin-producing Escherichia coli (SLTEC) in children with diarrhea. Methods We designed and synthesized 3 pairs of primers located in the SLT1, SLT2, and eaeA genes of enterohaemorrhagic Escherichia coli (EHEC), while the virulent genes SLT1, SLT2, and eaeA from E.coli species were amplified by polymerase chain reaction (PCR). Results One strain of EHEC with SLT1, SLT2, and eaeA in 29 reference strains of diarrhea-causing E.coli (DCEC) and 10 strains of other enterobacteria detected by PCR had positive reactions, while all other DCEC and enterobacteria were negative. Of 474 strains of E. coli isolated from 1032 children with diarrhea and detected by PCR, 20 strains of SLT1 producing E. coli (4.2%) positive, and 7 strains of SLT2 producing E.coli (1.5%) positive; while of 74 strains of entero-SLTs-producing and invasive Escherichia coli (ESIEC), 15 strains of SLT1 (20.3%) and 5 strains of SLT2 (6.8%) were positive. Conclusion Shiga-like toxin E. coli has been identified as a major etiologic agent of children with diarrhea in Taiyuan, China.