板栗下胚轴原生质体的分离与纯化

【目的】研究分离和纯化板栗下胚轴原生质体的影响因素。【方法】对分离板栗下胚轴原生质体的酶液组合、酶解时间、渗透压调节剂等因素进行优化,采用荧光增白剂(CFW)染色法和荧光素双醋酸酯(FDA)染色法分别对原生质体去壁和原生质体活力进行检测。【结果】分离板栗下胚轴原生质体最适宜的酶液组合为13%(w/v)甘露醇+2%(w/v)纤维素酶+0.5%(w/v)离析酶+1%(w/v)半纤维素酶,28℃温度下酶解6 h,原生质体产量达5.8×106个·g-1,且活力达94%。蔗糖浓度为23%(w/v)时,原生质体纯化效果最佳。【结论】建立了适合分离和纯化板栗下胚轴原生质体的方法。

Species of Heleanna Clarke, 1976(Lepidoptera: Tortricidae:Olethreutinae) from China

Two species of Heleanna Clarke are recorded in this paper. The genus Heleanna is first reported in Chinese mainland. H. turpinivora Nasu Byun, already known from Korea and Japan, is now reported in China. Variation in the female genitalia is described. A key to Chinese species of Heleanna is given.Photographs of the adults and genitalia of H. turpinivora are provided.