孵化酶是后生动物卵孵化过程中起关键作用的一类蛋白酶。基于生物信息学和RACE方法,从柞蚕胚胎中克隆了一个998 bp的孵化酶样基因全长cDNA,命名为ApHEL(GenBank登录号:JN205047)。ApHEL由15 bp 5'-UTR,98 bp 3'-UTR和885 bp OR...孵化酶是后生动物卵孵化过程中起关键作用的一类蛋白酶。基于生物信息学和RACE方法,从柞蚕胚胎中克隆了一个998 bp的孵化酶样基因全长cDNA,命名为ApHEL(GenBank登录号:JN205047)。ApHEL由15 bp 5'-UTR,98 bp 3'-UTR和885 bp ORF组成。ORF编码294个氨基酸残基,预测蛋白分子质量为33.51 kD,等电点为5.50。在ApHEL N-端存在16个氨基酸的信号肽。ApHEL蛋白酶功能区中含有锌结合序列(HEWMHILGFLHMQSTHNR)和Met-转角基序(YDYVSCLHY)等孵化酶保守功能区域。ApHEL与其他物种孵化酶氨基酸序列的相似度为30.0%～85.0%。基于孵化酶氨基酸序列的进化分析表明,柞蚕、家蚕、库蚊和果蝇聚为一类,亲缘关系较近。ApHEL在早期胚胎中没有转录,至催青第6天开始转录并维持较低水平,在催青第9天剧增至最高值直至孵化,显示ApHEL在柞蚕卵孵化过程中起重要作用。ApHEL在5龄期幼虫的中肠组织中特异性表达,暗示ApHEL可能还有其他生物学功能。展开更多
为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后...为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后3个区段的组织蛋白进行了比较分析.结果表明:中肠前、中、后3个区段组织蛋白的点数、位置、点形等差异较小.在中肠前部和后部组织中找出的4个差异蛋白点,分别为脂蛋白前体、囊膜氢离子泵ATP合酶B亚基、α胰蛋白酶A链和肌动蛋白;在中肠中部找出的一个差异蛋白点为Kun itz型胰蛋白酶抑制剂A链.这些蛋白点可能在BmDNV-ZJ侵染感受性家蚕中肠组织细胞中起一定的作用.展开更多
[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extru...[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.展开更多
文摘孵化酶是后生动物卵孵化过程中起关键作用的一类蛋白酶。基于生物信息学和RACE方法,从柞蚕胚胎中克隆了一个998 bp的孵化酶样基因全长cDNA,命名为ApHEL(GenBank登录号:JN205047)。ApHEL由15 bp 5'-UTR,98 bp 3'-UTR和885 bp ORF组成。ORF编码294个氨基酸残基,预测蛋白分子质量为33.51 kD,等电点为5.50。在ApHEL N-端存在16个氨基酸的信号肽。ApHEL蛋白酶功能区中含有锌结合序列(HEWMHILGFLHMQSTHNR)和Met-转角基序(YDYVSCLHY)等孵化酶保守功能区域。ApHEL与其他物种孵化酶氨基酸序列的相似度为30.0%～85.0%。基于孵化酶氨基酸序列的进化分析表明,柞蚕、家蚕、库蚊和果蝇聚为一类,亲缘关系较近。ApHEL在早期胚胎中没有转录,至催青第6天开始转录并维持较低水平,在催青第9天剧增至最高值直至孵化,显示ApHEL在柞蚕卵孵化过程中起重要作用。ApHEL在5龄期幼虫的中肠组织中特异性表达,暗示ApHEL可能还有其他生物学功能。
文摘为了探明浓核病毒镇江株(BmDNV-ZJ)感受性家蚕品种中肠组织不同部位对浓核病毒感受性差异的蛋白质组学机制,阐明家蚕浓核病毒的致病机理,应用等电聚焦(IFE),SDS-PAGE分离,2D P latinum软件以及质谱技术,对感受性家蚕品种中肠前、中、后3个区段的组织蛋白进行了比较分析.结果表明:中肠前、中、后3个区段组织蛋白的点数、位置、点形等差异较小.在中肠前部和后部组织中找出的4个差异蛋白点,分别为脂蛋白前体、囊膜氢离子泵ATP合酶B亚基、α胰蛋白酶A链和肌动蛋白;在中肠中部找出的一个差异蛋白点为Kun itz型胰蛋白酶抑制剂A链.这些蛋白点可能在BmDNV-ZJ侵染感受性家蚕中肠组织细胞中起一定的作用.
文摘[Objective] The aim of this study was to investigate the efficient technique of artificial insemination for silkworm. [Method] Sperms were extracted from bursa copulatrix of female moths mated for 30 min through extruding and centrifugal method, and then the semen was injected into other virgin moths with trypsinase. [ Result] A high-effective collection technology of spermatids from silkworm was established successfully, 50 μl semen could be collected by only one person in each hour. The survival rate of spermatids was over 80% in vito after collected from bursa copulatrix, while the obtained semen was quite pure and the average fertilization rate of silkworm was 76,5%. [ Conclusion] The establishment of high-effective semen extraction technique of silkworm provides the technical basis for studies on other related techniques for silkworm sperm.
