[Objective] This study was to investigate the effect of forsythiaside A added in chick embryo kidney cells (CEKs), on the changes in expression of interferon α(IFN-α) and mRNA expression level of correlated factors ...[Objective] This study was to investigate the effect of forsythiaside A added in chick embryo kidney cells (CEKs), on the changes in expression of interferon α(IFN-α) and mRNA expression level of correlated factors in JAK-STAT pathway. [Methods] Three levels of forsythiaside (100, 200, 400 μg/ml) were adopted to treat the experimental materials, then the expression levels of IFN-α and correlated factors in JAK-STAT pathway were detected by real-time RT-PCR at the transcriptional level (mRNA) and by Western blot at the translation level(IFN-α protein). [Result] Compared with the control group (untreated group), Application of forsythiaside A not only significantly increased the expression of IFN-α in treated CEKs (P<0.05) , but also up-regulated the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1 and IRF7, the correlated factors in JAK-STAT pathway. [Conclusion] Forsythiaside A induced the expression of IFN-α in CEKs, and can positively regulate the JAK-STAT signaling pathway significantly.展开更多
文摘[Objective] This study was to investigate the effect of forsythiaside A added in chick embryo kidney cells (CEKs), on the changes in expression of interferon α(IFN-α) and mRNA expression level of correlated factors in JAK-STAT pathway. [Methods] Three levels of forsythiaside (100, 200, 400 μg/ml) were adopted to treat the experimental materials, then the expression levels of IFN-α and correlated factors in JAK-STAT pathway were detected by real-time RT-PCR at the transcriptional level (mRNA) and by Western blot at the translation level(IFN-α protein). [Result] Compared with the control group (untreated group), Application of forsythiaside A not only significantly increased the expression of IFN-α in treated CEKs (P<0.05) , but also up-regulated the expression of STAT1, JAK1, IFNAR1, IFNAR2, IRF1 and IRF7, the correlated factors in JAK-STAT pathway. [Conclusion] Forsythiaside A induced the expression of IFN-α in CEKs, and can positively regulate the JAK-STAT signaling pathway significantly.
