The mechanisms underlying the response of soil respiration(Rs) to nitrogen(N) addition remain to be explored in semiarid ecosystems. This study was conducted to determine the effect of N addition on soil microbial com...The mechanisms underlying the response of soil respiration(Rs) to nitrogen(N) addition remain to be explored in semiarid ecosystems. This study was conducted to determine the effect of N addition on soil microbial composition, Rs and the temperature sensitivity of Rs(Q10). The N addition experiment was carried out in a semiarid grassland in China, with N fertilizer application rates of 0, 2, 4, 8, 16, or 32 gN m-2yr-1. Microbial phospholipid fatty acids(PLFAs), Rs and Q10 were measured, and their relationships with soil properties were determined for three growing seasons. The results showed that N addition significantly increased the content of soil dissolved organic carbon(DOC) and inorganic nitrogen(IN), and decreased soil p H. With respect to soil microbes, N addition reduced soil PLFAs, reduced the fungi to bacteria ratio(F:B) and increased the gram-positive bacteria to gram-negative bacteria ratio(G+:G–). Rs under the N2, N4, N8, N16 and N32 treatments decreased by 2.58%, 14.86%, 22.62%, 23.97% and 19.87%, respectively, compared to the N0(control) treatment. The results of structural equation models showed that N addition reduced Rs by lowering soil PLFAs and altering the microbial composition. However, N addition had no significant effect on either Q10, soil total organic carbon(TOC) or total nitrogen(TN), indicating that N addition alleviated soil carbon loss and was unlikely to change the potential for a bigger loss under global warming.展开更多
The priming effect is well acknowledged in soil systems but the effect of nitrogen(N)fertilization remains elusive.To explore how N modifies the priming effect in soil organic matter(SOM),one in situ experiment with 1...The priming effect is well acknowledged in soil systems but the effect of nitrogen(N)fertilization remains elusive.To explore how N modifies the priming effect in soil organic matter(SOM),one in situ experiment with 13C labeled glucose addition(0.4 mg C g^–1 soil,3.4 atom %^13C)was conducted on soil plots fertilized with three gradients of urea(0,4 and 16 g N m^–2 yr^–1).After glucose addition,the soil CO2 concentration and phospholipid fatty acid(PLFA)were measured on day 3,7,21 and 35.The study found that N fertilization decreased soil CO2,PLFA and the fungi to bacteria ratio.Glucose triggered the strongest positive priming in soil at 0 g N m^–2 yr^–2,meanwhile N fertilization decreased SOM-derived CO2.Soil at 4 g N m^–2 yr^–2 released the largest amount of glucose-derived carbon(C),likely due to favorable nutrient stoichiometry between C and N.Stable microbial community biomass and composition during early sampling suggests"apparent priming"in this grassland.This study concludes that N fertilization inhibited soil priming in semi-arid grassland,and shifted microbial utilization of C substrate from SOM to added labile C.Diverse microbial functions might be playing a crucial role in soil priming and requires attention in future N fertilization studies.展开更多
We investigated soil respiration(Rs)dynamics and influencing factors under different nitrogen(N)addition levels(0,2,4,8,16,32 g m^–2 yr^–1)on typical grassland plots in Inner Mongolia.We measured soil respiration,te...We investigated soil respiration(Rs)dynamics and influencing factors under different nitrogen(N)addition levels(0,2,4,8,16,32 g m^–2 yr^–1)on typical grassland plots in Inner Mongolia.We measured soil respiration,temperature,moisture and nutrients.We found that N addition did not change dynamic characteristics of Rs;daily and seasonal dynamics followed a single peak curve.N addition reduced Rs during the growing season.Rs under N2,N4,N8,N16 and N32 treatments decreased by 24.00%,21.93%,23.49%,30.78%and 28.20%in the growing season,respectively,compared to the N0 treatment.However,Rs in the non-growing season was not different across treatments.Rs was significantly positively correlated with soil temperature and moisture and these two factors accounted for 72%–97% and 74%–82% of variation in Rs,respectively.The soil respiration temperature sensitivity (Q10) was between 2.27 and 4.16 and N addition reduced Q10 except in the N8 treatment.展开更多
Urban surface soil has a unique set of structures and processes that affect surface soil organic carbon density(SOCdensity) and its spatial variations. Using Beijing as a case study, and assisted by field investigatio...Urban surface soil has a unique set of structures and processes that affect surface soil organic carbon density(SOCdensity) and its spatial variations. Using Beijing as a case study, and assisted by field investigations and experiments, we analyzed the spatial distribution of SOCdensity in different land use types and functional regions, and assessed associated factors such as urbanization level, the physiochemical properties of soil and plant configurations. The present study aims to provide useful information about the mechanisms driving soil organic carbon and climate change in developing and developed areas in urbanized regions like Beijing. Results indicate that P is the main factor positively influencing SOCdensity in most regions. Because of the specific interference directly related to human beings in urban areas, with decreases in the urbanization level, more physiochemical factors of soil can influence SOCdensity. SOCdensity under grasses is not significantly different from that under other plant compositions. Urbanization processes decrease the heterogeneity of the spatial pattern of SOCdensity in most land use types, but increased its contents when the area reached a developed level in Beijing. More factors related to human interference and spatial variation of surface soil carbon storage, especially under impervious land in urban areas, should be considered in future studies.展开更多
基金The National Natural Science Foundation of China(31770519)The National Key Research and Development Program of China(2017YFC0503805).
文摘The mechanisms underlying the response of soil respiration(Rs) to nitrogen(N) addition remain to be explored in semiarid ecosystems. This study was conducted to determine the effect of N addition on soil microbial composition, Rs and the temperature sensitivity of Rs(Q10). The N addition experiment was carried out in a semiarid grassland in China, with N fertilizer application rates of 0, 2, 4, 8, 16, or 32 gN m-2yr-1. Microbial phospholipid fatty acids(PLFAs), Rs and Q10 were measured, and their relationships with soil properties were determined for three growing seasons. The results showed that N addition significantly increased the content of soil dissolved organic carbon(DOC) and inorganic nitrogen(IN), and decreased soil p H. With respect to soil microbes, N addition reduced soil PLFAs, reduced the fungi to bacteria ratio(F:B) and increased the gram-positive bacteria to gram-negative bacteria ratio(G+:G–). Rs under the N2, N4, N8, N16 and N32 treatments decreased by 2.58%, 14.86%, 22.62%, 23.97% and 19.87%, respectively, compared to the N0(control) treatment. The results of structural equation models showed that N addition reduced Rs by lowering soil PLFAs and altering the microbial composition. However, N addition had no significant effect on either Q10, soil total organic carbon(TOC) or total nitrogen(TN), indicating that N addition alleviated soil carbon loss and was unlikely to change the potential for a bigger loss under global warming.
基金National Natural Science Foundation of China(31770519)National Key Research and Development Program of China(2017YFC0503805)
文摘The priming effect is well acknowledged in soil systems but the effect of nitrogen(N)fertilization remains elusive.To explore how N modifies the priming effect in soil organic matter(SOM),one in situ experiment with 13C labeled glucose addition(0.4 mg C g^–1 soil,3.4 atom %^13C)was conducted on soil plots fertilized with three gradients of urea(0,4 and 16 g N m^–2 yr^–1).After glucose addition,the soil CO2 concentration and phospholipid fatty acid(PLFA)were measured on day 3,7,21 and 35.The study found that N fertilization decreased soil CO2,PLFA and the fungi to bacteria ratio.Glucose triggered the strongest positive priming in soil at 0 g N m^–2 yr^–2,meanwhile N fertilization decreased SOM-derived CO2.Soil at 4 g N m^–2 yr^–2 released the largest amount of glucose-derived carbon(C),likely due to favorable nutrient stoichiometry between C and N.Stable microbial community biomass and composition during early sampling suggests"apparent priming"in this grassland.This study concludes that N fertilization inhibited soil priming in semi-arid grassland,and shifted microbial utilization of C substrate from SOM to added labile C.Diverse microbial functions might be playing a crucial role in soil priming and requires attention in future N fertilization studies.
基金National Key Research and Development Program of China(2017YFC0503805)National Natural Science Foundation of China(31770519)
文摘We investigated soil respiration(Rs)dynamics and influencing factors under different nitrogen(N)addition levels(0,2,4,8,16,32 g m^–2 yr^–1)on typical grassland plots in Inner Mongolia.We measured soil respiration,temperature,moisture and nutrients.We found that N addition did not change dynamic characteristics of Rs;daily and seasonal dynamics followed a single peak curve.N addition reduced Rs during the growing season.Rs under N2,N4,N8,N16 and N32 treatments decreased by 24.00%,21.93%,23.49%,30.78%and 28.20%in the growing season,respectively,compared to the N0 treatment.However,Rs in the non-growing season was not different across treatments.Rs was significantly positively correlated with soil temperature and moisture and these two factors accounted for 72%–97% and 74%–82% of variation in Rs,respectively.The soil respiration temperature sensitivity (Q10) was between 2.27 and 4.16 and N addition reduced Q10 except in the N8 treatment.
基金The Key Projects of National Natural Science Foundation of China (41530747)The State Key Laboratory of Earth Surface Processes and Resources Ecology (2017-FX-01(1))。
文摘Urban surface soil has a unique set of structures and processes that affect surface soil organic carbon density(SOCdensity) and its spatial variations. Using Beijing as a case study, and assisted by field investigations and experiments, we analyzed the spatial distribution of SOCdensity in different land use types and functional regions, and assessed associated factors such as urbanization level, the physiochemical properties of soil and plant configurations. The present study aims to provide useful information about the mechanisms driving soil organic carbon and climate change in developing and developed areas in urbanized regions like Beijing. Results indicate that P is the main factor positively influencing SOCdensity in most regions. Because of the specific interference directly related to human beings in urban areas, with decreases in the urbanization level, more physiochemical factors of soil can influence SOCdensity. SOCdensity under grasses is not significantly different from that under other plant compositions. Urbanization processes decrease the heterogeneity of the spatial pattern of SOCdensity in most land use types, but increased its contents when the area reached a developed level in Beijing. More factors related to human interference and spatial variation of surface soil carbon storage, especially under impervious land in urban areas, should be considered in future studies.