植物激素和抗生素调节的转基因火炬松愈伤组织的生长和分化(英文)

包含质粒载体pBI12 1的农杆菌菌株LBA4 4 0 4被用于转化火炬松的成熟合子胚。质粒载体pBI12 1含有胭脂碱合成酶基因的启动子驱动的新霉素磷酸转移酶基因 (nptⅡ )和花椰菜花叶病毒的 35S启动子驱动的GUS基因。器官发生的转基因愈伤组织和转基因的再生植株已经获得 ,并经GUS组织化学染色、聚合酶链式反应和Southern杂交分析证实。植物激素 (BA IBA)和抗生素对转基因愈伤组织的生长和分化的影响被研究。 5 0 0mg L羧苄青霉素和2mg LBA、0 .5mg LIBA的组合导致转基因愈伤组织的生长增加 5 4 .2 % ,分化增加 4 5 .7%。 5 0 0mg LClaforan和 2mg LBA、0 .5mg LIBA的组合导致转基因愈伤组织的生长增加 4 0 .8% ,分化增加 38.7%。高浓度的植物激素和抗生素降低了转基因愈伤组织的生长和分化。实验结果有助于建立一个高效的农杆菌介导的火炬松遗传转化系统 ,也有助于未来针叶树的遗传转化研究。

Regulated Gene Expression with Promoters Responding to Inducers

Genetically engineered transgenic animals and plants have proven to be extremely useful for analyzing biochemical and developmental processes.Promoters responding to chemical inducers will be powerful tools for basic research in molecular biology and biotechnological applications.Various chemical inducible systems based on activation and inactivation of the target gene had been described.The transfer of regulatory elements from prokaryotes,insects,and mammals has opened new avenues to construct chemically inducible promoters that differ in their ability to regulate the temporal and spatial expression patterns,and this will dramatically increase the application of transgenic technology.This review provides an overview on regulation of gene expression,promoter activating systems,promoter inactivation systems,inducible gene over expression,and inducible anti suppression.